The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices

Journal of Biomedical Materials Research - Part A

Volumn 73, Issue 3, 2005, Pages 367-375

  Grant, M Helen ^a   Morgan, Caroline ^a   Henderson, Catherine ^a   Malsch, Günther ^b   Seifert, Barbara ^b   Albrecht, Wolfgang ^b   Groth, Thomas ^b  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^b INSTITUTE OF MATERIALS RESEARCH   (Germany)

Author keywords

Bioartificial liver devices; Cytochrome P450 dependent monooxygenation; Glutathione S transferase expression; Hepatocyte culture; Membranes

Indexed keywords

ACETONITRILE; AMINES; BIOLOGICAL ORGANS; BIOSYNTHESIS; ENZYME KINETICS; HYDROPHOBICITY; ORGANIC ACIDS; POLYMERIC MEMBRANES;

ANCHORAGE-DEPENDENT CELLS; HEPATOCYTE CULTURES; LIVER DEVICES; TAILOR-MADE MEMBRANES;

ANIMAL CELL CULTURE;

ACRYLONITRILE; BIOMATERIAL; COPOLYMER; CYTOCHROME P450; GLUTATHIONE TRANSFERASE; POLYMER;

ANIMAL CELL; ARTICLE; ARTIFICIAL LIVER; BIOARTIFICIAL ORGAN; BIOCOMPATIBILITY; CELL FUNCTION; CELL ISOLATION; CELL MEMBRANE; CELL STRUCTURE; CELL VIABILITY; CONFOCAL MICROSCOPY; GRAFT HYBRIDIZATION; HYDROPHILICITY; HYDROPHOBICITY; LIVER CELL CULTURE; MALE; NONHUMAN; PROTEIN EXPRESSION; RAT; SYNTHESIS;

ANIMALS; BIOCOMPATIBLE MATERIALS; CELL CULTURE TECHNIQUES; CELL SHAPE; CELL SURVIVAL; CELLS, CULTURED; CYTOCHROME P-450 CYP1A1; GLUTATHIONE TRANSFERASE; HEPATOCYTES; LIVER, ARTIFICIAL; MALE; MATERIALS TESTING; POLYMERS; RATS; RATS, SPRAGUE-DAWLEY; WETTABILITY;

EID: 18644365731     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/jbm.a.30306     Document Type: Article

References (44)

1. Watanabe FD, Mullon CJP, Hewitt WR, Arkadopolus N, Kahaku E, Eguchi S, Khalili T, Arnaout W, Shackleton CR, Rozga J, Solomon B, Demetriou AA. Clinical experience with a bioartificial liver in the treatment of severe liver failure. A phase 1 clinical trial. Ann Surg 1997;225:484-494.
2. Busse B, Gerlach J, Muller C, Heufft HG, Klupp J, Shultensack U, Neuhaus P. Extracorporeal hybrid liver support system: bridging to liver transplantation (OLT) in a 45 year old female with drug induced fulminant hepatic failure (FHF). XXIIIrd Congress of ESAO, 1996. Warsaw. Wichtig Editore; 1996. p 544.
3. Legallais C, David B, Dore E. Bioartificial livers (BAL): current technological aspects and future developments. J Membr Sci 2000;181:81-95.
4. LeCluyse EL, Bullock PL, Parkinson A. Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Adv Drug Delivery Rev 1996;22:133-186.
5. Catapano G, Di Lorenzo MC, Volpe CD, De Bartolo L, Migliaresi C. Polymeric membranes for hybrid liver support devices: the effect of membrane surface wettability on hepatocyte viability and functions. J Biomater Sci Polym Ed 1996;7:1017-1027.
6. De Bartolo L, Catapano G, Volpe CD, Drioli E. The effect of surface roughness of microporous membranes on the kinetics of oxygen consumption and ammonia elimination by adherent hepatocytes. J Biomater Sci Polym Ed 1999;10:641-655.
7. Krasteva N, Groth T, Fey-Lamprecht F, Altankov G. The role of surface wettability on hepatocyte adhesion and function. J Biomater Sci Polym Ed 2001;12:613-627.
8. De Bartolo L, Morelli S, Rende M, Gordano E, Drioli E. New modified polyetheretherketone membrane for liver cell culture in biohybrid systems: adhesion and specific functions of isolated hepatocytes. Biomaterials 2004;25:3621-3629.
9. De Bartolo L, Morelli S, Bader A, Drioli E. The influence of polymeric membrane surface free energy on cell metabolic functions. J Mater Sci Mater Med 2001;12:959-963.
10. Iredale JP, Arthur MJP. Hepatocyte-matrix interactions. Gut 1994;35:304-316.
11. Dunn JCY, Tompkins RG, Yarmush ML. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog 1991;7:237-245.
12. LeCluyse EL, Audus KL, Hochman JH. Formation of extensive canalicular networks by rat hepatocytes cultured in collagen-sandwich configuration Am J Physiol 1994;266:C1764-C1774.
13. Nyberg SL, Shatford RA, Payne WD, Hu W-S, Cerra FB. Staining with fluorescein diacetate correlates with hepatocyte function. Biotechnol Histochem 1993;68:56-63.
14. Grant MH, Melvin MAL, Shaw P, Melvin WT, Burke MD. Studies on the maintenance of cytochromes P450 and b5, monooxygenases and cytochrome reductases in primary cultures of rat hepatocytes. FEBS Lett 1985;190:99-103.
15. Paine AJ. The maintenance of cytochrome P450 in rat hepatocyte culture: some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P450 system. Chem-Biol Interact 1990;74:1-31.
16. Utesch D, Molitor E, Platt KL, Oesch F. Differential stabilization of cytochrome P450 isoenzymes in primary cultures of adult rat liver parenchymal cells. In Vitro Cell Dev Biol 1991;27A:858-863.
17. Wortelboer HM, de Kruif CA, van Iersel AAJ, Falke HE, Noordhoek J, Blaauboer BJ. The isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme activities in microsomal incubations and in intact monolayers. Biochem Pharmacol 1990;40:2525-2534.
18. Burke MD, Thompson S, Weaver RJ, Wolf CR, Mayer RT. Cytochrome P450 specificities of alkoxyresorufin O-dealkylation in human and rat liver. Biochem Pharmacol 1994;48:923-936.
19. Guzelian PS, Li D, Schuetz EG. Induction of cytochromes P450b/e by phenobarbital in primary culture of adult rat hepatocytes: test of differentiated liver gene expression. Drug Metab Rev 1989;20:793-809.
20. MacDonald C, Vass M, Willett B, Scott A, Grant MH. Expression of liver functions in immortalised rat hepatocyte cell lines. Hum Exp Toxicol 1994;13:439-444.
21. Vandenberghe Y, Foriers A, Rogiers V, Vercruysse A. Changes in expression and 'de novo' synthesis of glutathione S-transferase subunits in cultured adult rat hepatocytes. Biochem Pharmacol 1990;39:685-690.
22. Vandenberghe Y, Tee L, Rogiers V, Yeoh G. Transcriptional-and post-translational-dependent regulation of glutathione S-transferase expression in rat hepatocytes as a function of culture conditions. FEBS Lett 1992;313:155-159.
23. Groth T, Seifert B, Malsch G, Albrecht W, Paul D, Kostadinova A, Krasteva N, Altankov G. Interaction of human fibroblasts with moderate wettable polyacrylonitrile-copolymer membranes. J Biomed Mater Res 2002;62:290-300.
24. Moldeus P, Hogberg J, Orrenius S. Isolation and use of liver cells. Methods Enzymol 1978;52:60-71.
25. Elsdale T, Bard J. Collagen substrata for studies on cell behaviour. J Cell Biol 1972;54:626-637.
26. McKay GC, Henderson C, Goldie E, Connel G, Westmoreland C, Grant MH. Cryopreservation of rat hepatocyte monolayers: cell viability and cytochrome P450 content in post-thaw cultures. Toxicol in Vitro 2002;16:71-79.
27. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
28. Watts P, Smith MD, Edwards I, Zammit V, Brown V, Grant MH. The influence of medium composition on the maintenance of cytochrome P450, glutathione content and urea synthesis: a comparison of rat and sheep primary hepatocyte cultures. J Hepatol 1995;23:1-8.
29. Omura T, Sato R. The carbon monoxide binding pigment of liver microsomes. J Biol Chem 1964;239:2370-2385.
30. Grant MH, Duthie SJ, Gray AG, Burke MD. Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line. Biochem Pharmacol 1988;37:4111-4116.
31. Grant MH, Anderson K, McKay G, Wills M, Henderson C, MacDonald C. Manipulation of the phenotype of immortalised rat hepatocytes by different culture configurations and by dimethylsulphoxide. Hum Exp Toxicol 2000;19:309-317.
32. Sonderfan AJ, Arlotto MP, Dutton DR, McMillen SK, Parkinson A. Regulation of testosterone hydroxylation by rat liver microsomal cytochrome P450. Arch Biochem Biophys 1987;255:27-41.
33. Hayes JD, Mantle TJ. Use of immuno-blot techniques to discriminate between the glutathione S-transferase Yf, Yk, Ya, Yn/Yb and Yc subunits and to study their distribution in extrahepatic tissues. Biochem J 1986;233:779-788.
34. Hayes JD, Nguyen T, Judah DJ, Peterson DG, Neal GE. Cloning of cDNAs from fetal rat liver encoding glutathione S transferase Yc polypeptides. J Biol Chem 1994;269:20707-20717.
35. Krasteva N, Seifert B, Hopp M, Malsch G, Albercht W, Altankov G, Groth T. Membranes for biohybrid liver support: the behaviour of C3A hepatoblastoma cells is dependent on the composition of acrylonitrile copolymers. J Biomater Sci Polym Ed 2005. Forthcoming.
36. Bissell DM, Stramatoglou SC, Nermut MV, Hughes RC. Interaction of rat hepatocytes with type IV collagen, fibronectin and laminin matrices. Distinct matrix-controlled modes of attachment and spreading. Eur J Cell Biol 1986;40:72-78.
37. De Bartolo L, Morelli S, Bader A, Drioli E. Evaluation of cell behaviour related to physico-chemical properties of polymeric membranes to be used in bioartificial organs. Biomaterials 2002;23:2485-2497.
38. Mills JC, Stone NL, Pittman RN. Extranuclear apoptosis: the role of the cytoplasm in the execution phase. J Cell Biol 1999;146:703-706.
39. Krasteva N, Harms U, Albrecht W, Seifert B, Hopp M, Altankov G, Groth T. Membranes for biohybrid liver support systems: investigations on hepatocyte morphology and growth. Biomaterials 2002;23:3467-3478.
40. Honiger J, Sarki R, Baudrimont M, Deleo R, Chafai N, Benoist S, Sarkis K, Ballader P, Capeau J, Nordlinger B. Semiautomatic macroencapsulation of large numbers of porcine hepatocytes by coextrusion with a solution of AN69 polymer. Biomaterials 2000;21:1269-1274.
41. Schenkman JB, Thummel KE, Favreau LV. Physiological and patho-physiological alterations in rat hepatic cytochrome P450. Drug Metab Rev 1989;20:557-584.
42. Steward AR, Dannan GA, Guzelian PS, Guengerich FP. Changes in the concentration of seven forms of cytochrome P450 in primary cultures of adult rat hepatocytes. Mol Pharmacol 1985;27:125-132.
43. Maier P, Saad B, Schawalder HP. Effect of periportal- and centrilobular-equivalent oxygen tension on liver specific functions in long term rat hepatocyte cultures. Toxicol in Vitro 1994;8:423-435.
44. Langsch A, Bader A. Long term stability of phase I and phase II enzymes of porcine liver cells in flat membrane bioreactors. Biotechnol Bioeng 2001;76:115-125.