Proteomic profiling of human plasma exosomes identifies PPARγ as an exosome-associated protein

Biochemical and Biophysical Research Communications

Volumn 378, Issue 3, 2009, Pages 433-438

  Looze, Christopher ^a   Yui, David ^a   Leung, Lester ^a   Ingham, Matthew ^a   Kaler, Maryann ^a   Yao, Xianglan ^a   Wu, Wells W ^a   Shen, Rong Fong ^a   Daniels, Mathew P ^a   Levine, Stewart J ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Exosome; Lipoprotein; Peroxisome proliferator activated receptor ; Plasma

Indexed keywords

CELL PROTEIN; INTERMEDIATE DENSITY LIPOPROTEIN; LIPOPROTEIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; VERY LOW DENSITY LIPOPROTEIN;

ADIPOCYTE; ARTICLE; CELL DIFFERENTIATION; CELL FUNCTION; CELL PROLIFERATION; CENTRIFUGATION; CONTROLLED STUDY; EXOSOME; GEL CHROMATOGRAPHY; HUMAN; IMMUNOCOMPETENT CELL; INFLAMMATORY CELL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PLASMA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEOMICS; SUCROSE GRADIENT;

CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXOSOMES; HUMANS; LIPOPROTEINS, IDL; LIPOPROTEINS, VLDL; MASS SPECTROMETRY; PPAR GAMMA; PROTEIN ARRAY ANALYSIS; PROTEOMICS; SERUM;

EID: 57849103455     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.11.050     Document Type: Article

References (9)

1. Thery C., Zitvogel L., and Amigorena S. Exosomes: composition, biogenesis and function. Nat. Rev. Immunol. 2 (2002) 569-579
2. van Niel G., Porto-Carreiro I., Simoes S., and Raposo G. Exosomes: a common pathway for a specialized function. J. Biochem. (Tokyo) 140 (2006) 13-21
3. Caby M.P., Lankar D., Vincendeau-Scherrer C., Raposo G., and Bonnerot C. Exosomal-like vesicles are present in human blood plasma. Int. Immunol. 17 (2005) 879-887
4. Zhang J., Hawari F.I., Shamburek R.D., Adamik B., Kaler M., Islam A., Liao D.W., Rouhani F.N., Ingham M., and Levine S.J. Circulating TNFR1 exosome-like vesicles partition with the LDL fraction of human plasma. Biochem. Biophys. Res. Commun. 366 (2008) 579-584
5. Wang G., Wu W.W., Zeng W., Chou C.L., and Shen R.F. Label-free protein quantification using LC-coupled ion trap or FT mass spectrometry: reproducibility, linearity, and application with complex proteomes. J. Proteome Res. 5 (2006) 1214-1223
6. C. Thery, S. Amigorena, G. Raposo, A. Clayton, isolation and characterization of exosomes from cell culture supernatants and biological fluids, Curr. Protoc. Cell Biol. (2006) (Chapter 3, Unit 3.22).
7. Anderson L. Candidate-based proteomics in the search for biomarkers of cardiovascular disease. J. Physiol. 563 (2005) 23-60
8. Raiborg C., Bache K.G., Gillooly D.J., Madshus I.H., Stang E., and Stenmark H. Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes. Nat. Cell. Biol. 4 (2002) 394-398
9. Heikkinen S., Auwerx J., and Argmann C.A. PPARgamma in human and mouse physiology. Biochim. Biophys. Acta 1771 (2007) 999-1013