Genetic characterization of two putative toxin-antitoxin systems on cryptic plasmids from Bacillus thuringiensis strain YBT-1520

Journal of Microbiology and Biotechnology

Volumn 18, Issue 10, 2008, Pages 1630-1633

  Liu, Xiaojin ^a   Zhu, Shufang ^a   Ye, Weixing ^a   Ruan, Lifang ^a   Yu, Ziniu ^a   Zhao, Changming ^a   Sun, Ming ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bacillus thuringiensis; Segregational stability toxin antitoxin system; Small cryptic plasmids

Indexed keywords

AMINO ACID; ANTITOXIN; BACILLUS THURINGIENSIS TOXIN; BMB0031 TOXIN; BMB0032 TOXIN; BMB0631 TOXIN; BMB1182 TOXIN; BMB7635 TOXIN; BMB8240 TOXIN; BMB9741 TOXIN; PROTEIN KYA; PROTEIN KYB; PROTEIN TASA; PROTEIN TASB; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL STRAIN; DNA TEMPLATE; GENE SEGREGATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC IDENTIFICATION; GENETIC SIMILARITY; GENETIC STABILITY; LETHAL GENE; LETHALITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

ANTITOXINS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; ESCHERICHIA COLI; PLASMIDS;

BACILLUS THURINGIENSIS; ESCHERICHIA COLI;

EID: 57849103056     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Anantharaman, V. and L. Aravind. 2003. New connections in the prokaryotic toxin-antitoxin network: Relationship with the eukaryotic nonsense-mediated RNA decay system. Genome Biol. 4: R81.
2. Andrup, L., G. B. Jensen, A. Wilcks, L. Smidt, L. Hoflack, and J. Mahillon. 2003. The patchwork nature of rolling-circle plasmids: Comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid 49: 205-232.
3. Baum, J. A. 1994. Tn5401, a new class II transposable element from Bacillus thuringiensis. J. Bacteriol. 176: 2835-2845.
4. Choi, J. Y., M. S. Li, H. J. Shim, J. Y. Roh, S. D. Woo, B. R. Jin, K. S. Boo, and Y. H. Je. 2007. Isolation and characterization of strain of Bacillus thuringiensis subsp. kenyae containing two novel cry1-type toxin genes. J. Microbiol. Biotechnol. 17: 1498-1503.
5. Dziewit, L., M. Jazurek, L. Drewniak, J. Baj, and D. Bartosik. 2007. The SXT conjugative element and linear prophage N15 encode toxin-antitoxin-stabilizing systems homologous to the tad-ata module of the Paracoccus aminophilus plasmid pAMI2. J. Bacteriol. 189: 1983-1997.
6. Fico, S. and J. Mahillon. 2006. TasA-tasB, a new putative toxin- antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system. BMC Genomics 7: 259.
7. Gerdes, K., S. K. Christensen, and A. Lobner-Olesen. 2005. Prokaryotic toxin-antitoxin stress response loci. Nat. Rev. Microbiol. 3: 371-382.
8. Grady, R. and F. Hayes. 2003. Axe-Txe, a broad-spectrum proteic toxin-antitoxin system specified by a multidrug-resistant, clinical isolate of Enterococcus faecium. Mol. Microbiol. 47: 1419-1432.
9. Guerout-Fleury, A. M., K. Shazand, N. Frandsen, and P. Stragier. 1995. Antibiotic-resistance cassettes for Bacillus subtilis. Gene 167: 335-336.
10. Hayes, F. 2003. Toxins-antitoxins: Plasmid maintenance programmed cell death and cell cycle arrest. Science 301: 1496-1499.
11. Li, L., C. Yang, Z. Liu, F. Li, and Z. Yu. 2000. Screening of acrystalliferous mutants from Bacillus thuringiensis and their transformation properties. Wei Sheng Wu Xue Bao 40: 85-90. (In Chinese.)
12. Li, M. S., J. Y. Choi, J. Y. Roh, H. J. Shim, J. N. Kang, Y. S. Kim, et al. 2007. Identification and molecular characterization of novel cry1-type toxin genes from Bacillus thuringiensis K1 isolated in Korea. J. Microbiol. Biotechnol. 17: 15-20.
13. Mahillon, J., F. Hespel, A. M. Pierssens, and J. Delcour. 1988. Cloning and partial characterization of three small cryptic plasmids from Bacillus thuringiensis. Plasmid 19: 169-173.
14. Oberer, M., K. Zangger, S. Prytulla, and W. Keller. 2002. The antitoxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins. Biochem. J. 361: 41-47.
15. Ogata, H., P. Renesto, S. Audic, C. Robert, G. Blanc, P. E. Fournier, H. Parinello, J. M. Claverie, and D. Raoult. 2005. The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite. PLoS. Biol. 3: e248.
16. Pandey, D. P. and K. Gerdes. 2005. Toxin-antitoxin quality control loci are highly abundant in free-living but lost from host- associated prokaryotes. Nucleic Acids Res. 33: 966-976.
17. Raumann, B. E., M. A. Rould, C. O. Pabo, and R. T. Sauer. 1994. DNA recognition by β-sheets in the Arc repressor-operator crystal structure. Nature 367: 754-757.
18. Roh, J. Y., J. Y. Choi, M. S. Li, B. R. Jin, and Y. H. Je. 2007. Bacillus thuringiensis as a specific, safe, and effective tool for insect pest control. J. Microbiol. Biotechnol. 17: 547-559.
19. Sanchis, V., H. Agaisse, J. Chaufaux, and D. Lereclus. 1997. A recombinase-mediated system for elimination of antibiotic resistance gene markers from genetically engineered Bacillus thuringiensis strains. Appl. Environ. Microbiol. 63: 779-784.
20. Sun, M., Z. Liu, and Z. Yu. 2000. Characterization of the insecticidal crystal protein genes of Bacillus thuringiensis YBT-1520. Wei Sheng Wu Xue Bao 40: 365-371. (In Chinese.)
21. Wilcks, A., L. Smidt, O. A. Okstad, A. B. Kolsto, J. Mahillon, and L. Andrup. 1999. Replication mechanism and sequence analysis of the replicon of pAW63, a conjugative plasmid from Bacillus thuringiensis. J. Bacteriol. 181: 3193-3200.
22. Zhang, Q., M. Sun, Z. Xu, and Z. Yu. 2007. Cloning and characterization of pBMB9741, a native plasmid of Bacillus thuringiensis subsp. kurstaki strain YBT-1520. Curr. Microbiol. 55: 302-307.