Identification and molecular characterization of novel cry1-type toxin genes from Bacillus thuringiensis K1 isolated in Korea

Journal of Microbiology and Biotechnology

Volumn 17, Issue 1, 2007, Pages 15-20

  Li, Ming Shun ^a   Choi, Jae Young ^b   Roh, Jong Yul ^b   Shim, Hee Jin ^b   Kang, Joong Nam ^b   Kim, Yang Su ^b   Wang, Yong ^b   Yu, Zi Niu ^a   Jin, Byung Rae ^c   Je, Yeon Ho ^b  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b Seoul National University   (South Korea)

^c Dong A University   (South Korea)

Author keywords

Bacillus thuringiensis K1; Baculovirus expression vector system; Cry1 type gene; Insecticidal activity; Inverse PCR; RFLP

Indexed keywords

BACTERIAL PROTEIN; PROTEIN CRY1; UNCLASSIFIED DRUG;

ARMYWORM; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENETICS; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BACULOVIRUS EXPRESSION SYSTEM; GENE AMPLIFICATION; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEQUENCE; INSECTICIDAL ACTIVITY; KOREA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; BIOLOGICAL ASSAY; DNA PRIMERS; DNA, BACTERIAL; ENDOTOXINS; GENES, BACTERIAL; HEMOLYSIN PROTEINS; INSECTS; KOREA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

BACILLUS THURINGIENSIS; PLUTELLA XYLOSTELLA; SPODOPTERA EXIGUA;

EID: 33846872315     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Ben-Dov, E., A. Zaritsky, E. Dalian, Z. Barak, R. Sinai, R. Manasherob, A. Khamraev, E. Troitskaya, D. Dubitsky, N. Berezina, and Y. Margalith. 1997. Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis. Appl. Environ. Microbiol. 63: 4883-4890.
2. Chambers, J. A., A. Jelen, M. P. Gilbert, C. S. Jany, T. B. Johnson, and C. Gawron-Burke. 1991. Isolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai. J. Bacteriol. 173: 3966-3976.
3. Chang, J. H., J. Y. Choi, B. R. Jin, J. Y. Rob, J. A. Olszewski, S. J. Seo, D. R. O'Reilly, and Y. H. Je. 2003. An improved baculovirus insecticide producing occlusion bodies that contain Bacillus thuringiensis insect toxin. J. Inverbr. Pathol. 84: 30-37.
4. Choi, J. Y., M. S. Li, J. Y. Rob, H. J. Shim, K. S. Boo, and Y. H. Je. 2003. Identification of novel insecticidal cry1-type genes from Bacillus thuringiensis strains isolated in Korea. J. Asia-Pacific Entomol. 6: 229-233.
5. Crickmore, N., D. R. Zeigler, J. Feitelson, E. Schnepf, J. Van Rie, D. Lereclus, J. Baum, and D. H. Dean. 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev 62: 807-813.
6. Entwistle, F. P., S. J. Cory, J. M. Bailey, and S. Higgs. 1993. Bacillus thuringiensis, An Environmental Biopesticide: Theory and Practice, pp. 9-11. John Wiley and Sons, Chichester, England.
7. Finney, D. J. 1971. Probit Analysis, 3rd Ed. Cambridge University Press, U.K.
8. Gill, S. S., E. A. Cowlest, and V. Francis. 1995. Identification, isolation and cloning of a Bacillus thuringiensis CryIAc toxin-binding protein from midgut of the lepidopteran insect Heliothis virescens. J. Biol. Chem. 270: 27277-27282.
9. Horak, P., J. Weiser, and L. M. Scaron. 1996. The effect of Bacillus thuringiensis M-exotoxin of Trematode cercariae. J. Invertebr. Pathol. 68: 41-49.
10. Hwang, S. H., H. Saitoh, E. Mizuki, K. Higuchi, and M. Ohba. 1998. A novel class of mosquitocidal δ-endotoxin, Cry19B, encoded by a Bacillus thuringiensis serovar higo gene. Syst. Appl. Microbiol. 21: 179-184.
11. Je, Y. H., B. R. Jin, H. W. Park, J. Y. Rob, J. H. Chang, and S. K. Kang. 1999. Construction of a novel recombinant baculovirus producing polyhedra with a Bacillus thuringiensis Cry1Ac crystal protein. J. Korean Soc. Virol. 29: 145-153.
12. Je, Y. H., B. R. Jin, J. Y. Roh, J. H. Chang, S. J. Seo, J. A. Olszewski, D. R. O'Reilly, and S. K. Kang. 2003. Baculovirus expression vectors that incorporate the foreign protein into viral occlusion bodies. Biotechniques 34: 81-87.
13. Johnson, C., A. H. Bishop, and C. L. Turner. 1998. Isolation and activity of strains of Bacillus thuringiensis toxic to larvae of the housefly (Diptera: Muscidae) and tropical blowflies (Diptera: Calliphoridae). J. Invertebr. Pathol. 71: 138-144.
14. Jung, S.-Y., J.-H. Lee, Y.-G. Chai, and S.-J. Kim. 2005. Monitoring of microorganisms added into oil-contaminated microenvironments by terminal-restriction fragment length polymorphism analysis. J. Microbiol. Biotechnol. 15: 1170-1177.
15. Kim, H. S., D. W. Lee, S. D. Woo, Y. M. Yu, and S. K. Kang. 1998. Distribution, serological identification, and PCR analysis of Bacillus thuringiensis isolated from soils of Korea. Curr. Microbiol. 89: 16-23.
16. Kim, J.-N., S.-J. Lee, H.-S. Lee, and H.-G. Rhie. 2005. Inactivation of mutS leads to a multiple-drug resistance in Pseudomonas putida ATCC12633. J. Microbiol. Biotechnol. 15: 1214-1220.
17. Kim, J. S., J. Y. Choi, J. H. Chang, H. J. Shim, J. Y. Roh, B. R. Jin, and Y. H. Je. 2005. Characterization of an improved recombinant baculovirus producing polyhedra that contain Bacillus thuringiensis Cry1Ac crystal protein. J. Microbiol. Biotechnol. 15: 710-715.
18. Kim, W.-K., R. C., and D. Jahng. 2005. Enrichment of ammonia-oxidizing bacteria for efficient nitrification of wastewater. J. Microbiol. Biotechnol. 15: 772-779.
19. Kondo, S., N. Tamura, A. Kunitate, M. Hattori, A. Akashi, and I. Ohmori. 1987. Cloning and nucleotide sequencing of two insecticidal delta-endotoxin genes from Bacillus thuringiensis var. kurstaki HD-1 DNA. Agric. Biol. Chem. 51: 455-463.
20. Koo, B. T., S. H. Park, S. K. Choi, B. S. Shin, J. I. Kim, and J. H. Yu. 1995. Cloning of a novel crystal protein gene cry1K from Bacillus thuringiensis subsp. morrisoni. FEMS Microbiol. Lett. 134: 159-164.
21. Kuo, W. S. and K. F. Chak. 1996. Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA. Appl. Environ. Microbiol. 62: 1369-1377.
22. Li, M. S., Y. H. Je, I. H. Lee, J. H. Chang, J. Y. Roh, H. S. Kim, H. W. Oh, and K. S. Boo. 1995. Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new δ-endotoxins gene. Curr. Microbiol. 45: 299-302.
23. Moar, W. J., M. Pusztai-Carey, H. Van Faassen, D. Bosch, R. Frutos, C. Rang, K. Luo, and M. J. Adang. 1995. Development of Bacillus thuringiensis Cry1C resistance by Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae). Appl. Environ. Microbiol. 61: 2086-2092.
24. Ohba, M. and K. Aizawa. 1978. Serological identification of Bacillus thuringiensis and related bacteria isolated in Japan. J. Invertebr. Pathol. 32: 303-309.
25. O'Reilly, D. R., L. K. Miller, and V. A. Luckow. 1992. Baculovirus Expression Vectors: A Laboratory Manual. Oxford University Press, New York.
26. Pietrantonio, P. V. and S. S. Gill. 1992. The parasporal inclusion of Bacillus thuringiensis subp. shandongiensis: Characterization and screening for insecticidal activity. J. Invertebr. Pathol. 59: 295-302.
27. Schnepf, H. E. and H. R. Whiteley. 1981. Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. Proc. Natl. Acad. Sci. USA 78: 2893-2897.
28. Spizizen, J. 1958. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. USA 44:1072-1078.
29. Tounsi, S., A. J'Mal, N. Zouari, and S. Jaoua. 1999. Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp. kurstaki. Biotechnol. Lett. 21: 771-775.
30. Visser, B., E. Munsterman, A. Stocker, and W. G. Dirkse. 1990. A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific crystal protein. J. Bacteriol. 172: 6783-6788.
31. Von Tersch, M. A., H. L. Robbins, C. S. Jany, and T. B. Johnson. 1991. Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: Gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki Cry1A(c) toxins. Appl. Environ. Microbiol. 57: 349-358.
32. Walters, F. S. and L. H. English. 1995. Isolation and characterization of a strain of Bacillus thuringiensis δ-endotoxins toward the potato aphid in an artificial diet bioassay. Entomol. Exp. Appl. 77: 211-216.