A novel single-cell quantitative real-time RT-PCR method for quantifying foot-and-mouth disease viral RNA

Journal of Virological Methods

Volumn 155, Issue 2, 2009, Pages 150-156

  Huang, Xuan ^a   Li, Yong ^a   Zheng, Cong yi ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Acute infection; Foot and mouth disease virus; Single cell quantitative real time RT PCR

Indexed keywords

VIRUS RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOLYSIS; FOOT AND MOUTH DISEASE VIRUS; MICROMANIPULATOR; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA STABILITY; VIRUS GENOME; VIRUS INFECTIVITY;

ANIMALS; CELL LINE; CRICETINAE; CYTOPATHOGENIC EFFECT, VIRAL; FOOT-AND-MOUTH DISEASE VIRUS; KIDNEY; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

FOOT-AND-MOUTH DISEASE VIRUS; RNA VIRUSES;

EID: 57749204125     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.10.007     Document Type: Article

References (34)

1. Alexandersen S., and Mowat G.N. Foot-and-mouth disease: host range and pathogenesis. Curr. Top. Microbiol. Immunol. 288 (2005) 9-42
2. Alexandersen S., Zhang Z., Donaldson A.I., and Garland A.J. The pathogenesis and diagnosis of foot-and-mouth disease. J. Comp. Pathol. 129 (2003) 1-36
3. Alsbo C.W., Wrang M.L., Moller F., and Diemer N.H. Is the AMPA receptor subunit GluR2 mRNA an early indicator of cell fate after ischemia? A quantitative single cell RT-PCR study. Brain Res. 894 (2001) 101-108
4. Belsham G.J. Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family: aspects of virus protein synthesis, protein processing and structure. Prog. Biophys. Mol. Bio. 60 (1993) 241-260
5. Callahan J.D., Brown F., Osorio F.A., Sur J.H., Kramer E., Long G.W., Lubroth J., Ellis S.J., Shoulars K.S., Gaffney K.L., Rock D.L., and Nelson W.M. Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. J. Am. Vet. Med. Assoc. 220 (2002) 1636-1642
6. Cleaves G.R., Ryan T.E., and Schlesinger R.W. Identification and characterization of type 2 dengue virus replicative intermediate and replicative form RNAs. Virology 111 (1981) 73-83
7. De la Torre J.C., Dávila M., Sobrino F., Ortín J., and Domingo E. Establishment of cell lines persistently infected with foot-and-mouth disease virus. Virology 145 (1985) 24-35
8. Domingo E., Escarmís C., Baranowski E., Ruiz-Jarabo C.M., Carrillo E., Núñez J.I., and Sobrino F. Evolution of foot-and-mouth disease virus. Virus Res. 91 (2003) 47-63
9. Fosgate, G.T., Tavornpanich, S., Hunter, D., Pugh, R., Sterle, J.A., Schumann, K.R., Eberling, A.J., Beckham, T.R., Martin, B.M., Clarke, N.P., Adams, L.G., 2008. Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection. Vet. Microbiol. (Epub ahead of print).
10. Grubman M.J., and Baxt B. Foot-and-mouth disease. Clin. Microbiol. Rev. 17 (2004) 465-493
11. Gu C., Zheng C., Wu J., Qu S., Yang W., and Chang H. Rapid selection of the cells persistently infected with foot-and-mouth disease virus and the study of natures in persistent infection cells. Virologica Siniga 18 (2003) 459-463
12. Gu C., Zheng C., Shi L., Zhang Q., Li Y., Lu B., Xiong Y., Qu S., Shao J., and Chang H. Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Virus Genes 34 (2007) 2461-2469
13. Hartshorn C., Anshelevich A., and Wangh L.J. Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell. BMC Biotechnol. 5 (2005) 2
14. Hartshorn C., Eckert J.J., Hartung O., and Wangh L.J. Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos. BMC Biotechnol. 7 (2007) 87
15. Hillman K.L., Knudson C.A., Carr P.A., Doze V.A., and Porter J.E. Adrenergic receptor characterization of CA1 hippocampal neurons using real time single cell RT-PCR. Mol. Brain. Res. 139 (2005) 267-276
16. Hofmann M.A., Thür B., Liu L., Gerber M., Stettler P., Moser C., and Bossy S. Rescue of infectious classical swine fever and foot-and-mouth disease virus by RNA transfection and virus detection by RT-PCR after extended storage of samples in Trizol. J. Virol. Methods 87 (2000) 29-39
17. Horsington J., and Zhang Z. Analysis of foot-and-mouth disease virus replication using strand-specific quantitative RT-PCR. J. Virol. Methods 144 (2007) 149-155
18. Jemiolo B., and Trappe S. Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exercise. Biochem. Biophys. Res. Commun. 320 (2004) 1043-1050
19. Jeffreys A.J., Wilson V., Neumann R., and Keytel J. Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells. Nucleic. Acids. Res. 16 (1988) 10953-10971
20. Krapf R., and Solioz M. Na/H antiporter mRNA expression in single nephron segments of rat kidney cortex. J. Clin. Invest. 88 (1991) 783-788
21. King D.P., Ferris N.P., Shaw A.E., Reid S.M., Hutchings G.H., Giuffre A.C., Robida J.M., Callahan J.D., Nelson W.M., and Beckham T.R. Detection of foot-and-mouth disease virus: comparative diagnostic sensitivity of two independent real-time reverse transcription polymerase chain reaction assays. J. Vet. Diagn. Invest. 18 (2006) 93-97
22. Kullberg M., Nilsson M.A., Arnason U., Harley E.H., and Janke A. Housekeeping genes for phylogenetic analysis of Eutherian relationships. Mol. Biol. Evol. 23 (2006) 1493-1503
23. C receptor ρ subunits in rat hippocampal pyramidal and granule cells. Mol. Brain Res. 123 (2004) 1-6
24. Mattano Jr. L.A., Moss T.J., and Emerson S.J. Sensitive detection of rare circulating neuroblastoma cells by the reverse transcriptase polymerase chain reaction. Cancer. Res. 52 (1992) 4701-4705
25. Moss A., and Hass B. Comparison of the plaque test and reverse transcription nested PCR for detection of FMDV in nasal swabs and probing samples. J. Virol. Methods 80 (1999) 59-67
26. Parhar I.S., Ogawa S., Hamada T., and Sakuma Y. Single-cell real-time quantitative polymerase chain reaction of immunofluorescently identified neurons of gonadotropin-releasing hormone subtypes in cichlid fish. Endocrinology 144 (2003) 3297-3300
27. Pierce K.E., Rice J.E., Sanchez J.A., and Wangh L.J. QuantiLyse™: reliable DNA amplification from single cells. BioTechniques 32 (2002) 1106-1111
28. Pierce K.E., Rice J.E., Sanchez J.A., and Wangh L.J. Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR. Mol. Hum. Reprod. 9 (2003) 815-820
29. Ponnaiya B., Baker G.J., Pherson G.R., and Geard C.R. Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses. Exp. Hematol. 35 (2007) 64-68
30. Rice J.E., Sanchez J.A., Pierce K.E., and Wangh L.J. Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells. Prenat. Diagn. 22 (2002) 1130-1134
31. Shaw A.E., Reid S.M., Ebert K., Hutchings G.H., Ferris N.P., and King D.P. Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease. J. Virol. Methods 143 (2007) 81-85
32. Verheyden B., Lauwers S., and Rombaut B. Quantitative RT-PCR ELISA to determine the amount and ratio of positive- and negative-strand viral RNA synthesis and the effect of guanidine in poliovirus infected cells. J. Pharm. Biomed. Anal. 33 (2003) 303-308
33. Wacker, M.J., Tehel, M.M., Gallagher, P.M., 2008. Technique for quantitative RT-PCR analysis directly from single muscle fibers. J. Appl. Physiol. (Epub ahead of print).
34. Wagatsuma A., Sadamoto H., Kitahashi T., Lukowiak K., Urano A., and Ito E. Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR. J. Exp. Biol. 208 (2005) 2389-2398