Gene cloning and biochemical characterization of an alcohol dehydrogenase from Euglena gracilis

Journal of Eukaryotic Microbiology

Volumn 55, Issue 6, 2008, Pages 554-561

  Palma Gutiérrez, Helga N ^a   Rodríguez Zavala, José Salud ^a   Jasso Chávez, Ricardo ^a   Moreno Sánchez, Rafael ^a   Saavedra, Emma ^a  

^a INSTITUTO NACIONAL DE CARDIOLOGÍA IGNACIO CHÁVEZ   (Mexico)

Author keywords

ADH medium chain dehydrogenase reductase; Euglenozoa; Microbial alcohol metabolism

Indexed keywords

ALCOHOL DEHYDROGENASE; PROTOZOAL PROTEIN;

BACTERIUM; ENZYME ACTIVITY; ETHANOL; GENE; METABOLISM; PH; PROTOZOAN; SUBSTRATE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; EUGLENA GRACILIS; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT;

ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; EUGLENA GRACILIS; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; PROTOZOAN PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); EUGLENA GRACILIS; EUGLENOZOA; FUNGI;

EID: 57749202682     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2008.00359.x     Document Type: Article

References (37)

1. Ahmadinejad, N., Dagan, T. Martin, W. 2007. Genome history in the symbiotic hybrid Euglena gracilis. Gene, 402 : 35 39.
2. Bränden, C. I., Jörnvall, H., Eklund, H. Furugren, B. 1975. Alcohol dehydrogenases. In : Boyer, P. D. (ed.), The Enzymes. 3rd ed. Academic Press, New York. XI : 103 190.
3. Buetow, D. E. 1989. The mitochondrion. In : Buetow, D. E. (ed.), The Biology of Euglena. Academic Press, San Diego, CA. 4 : 247 313.
4. Bystrykh, L. V., Vonck, J., van Bruggen, E. F., van Beeumen, J., Samyn, B., Govorukhina, N. I., Arfman, N., Duine, J. A. Dijkhuizen, L. 1993. Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N′-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19. J. Bacteriol., 175 : 1814 1822.
5. Casadio, R., Martelli, P. L., Giordano, A., Rossi, M. Raia, C. A. 2002. A low-resolution 3D model of the tetrameric alcohol dehydrogenase from Sulfolobus solfataricus. Protein Eng., 15 : 215 223.
6. Cervantes, C., Espino-Saldaña, A. E., Acevedo-Aguilar, F., León-Rodriguez, I. L., Rivera-Cano, M. E., Avila-Rodríguez, M., Wróbel-Kaczmarczyk, K., Wróbel-Zasada, K., Gutiérrez- Corona, J. F., Rodríguez-Zavala, J. S. Moreno-Sánchez, R. 2006. Microbial interactions with heavy metals. Rev. Latinoam. Microbiol., 48 : 203 210.
7. Cook, J. R. 1968. The cultivation and growth of Euglena. In : Buetow, D. E. (ed.), The Biology of Euglena. Academic Press, New York. 1 : 243 314.
8. Danforth, W. F. Hunter, F. R. 1966. Isocitrate dehydrogenase and NADP-alcohol dehydrogenase of Euglena gracilis var. bacillaris. J. Protozool., 13 : 654 658.
9. Davis, B. Merrett, M. J. 1973. Malate dehydrogenase isoenzymes in division synchronized cultures of Euglena. Plant. Physiol., 51 : 1127 1132.
10. De Bolle, X., Vinals, C., Fastrez, J. Feytmans, E. 1997. Bivalent cations stabilize yeast alcohol dehydrogenase I. Biochem. J., 323 : 409 413.
11. Eshleman, J. N. Danforth, W. F. 1964. Some characteristics of ethanol metabolism in Euglena gracilis var bacillaris. J. Protozool., 11 : 394 399.
12. Ganzhorn, A. J., Green, D. W., Hershey, A. D., Gould, R. M. Plapp, B. V. 1987. Kinetic characterization of yeast alcohol dehydrogenases. J. Biol. Chem., 262 : 3754 3761.
13. Hallick, R. B., Hong, L., Drager, R. G., Favreau, M. R., Monfort, A., Orsat, B., Spielmann, A. Stutz, E. 1993. Complete sequence of Euglena gracilis chloroplast DNA. Nucleic Acids Res., 21 : 3537 3544.
14. Hektor, H. J., Kloosterman, H. Dijkhuizen, L. 2002. Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus. J. Biol. Chem., 277 : 46966 46973.
15. Henze, K., Badr, A., Wettern, M., Cerff, R. Martin, W. 1994. A nuclear gene of eubacterial origin in Euglena gracilis reflects cryptic endosymbioses during protist evolution. Proc. Natl. Acad. Sci. USA, 92 : 9122 9126.
16. Hurlbert, R. E. Rittenberg, S. C. 1962. Glucose metabolism of Euglena gracilis variety bacillaris; growth and enzymic studies. J. Protozool., 9 : 170 182.
17. Inui, H., Miyatake, K., Nakano, Y. Kitaoka, S. 1984. Fatty acid synthesis in mitochondria of Euglena gracilis. Eur. J. Biochem., 142 : 121 126.
18. Jasso-Chávez, R. Moreno-Sánchez, R. 2003. Cytosol-mitochondria transfer of reducing equivalents by a lactate shuttle in heterotrophic Euglena. Eur. J. Biochem., 270 : 4942 4951.
19. Jasso-Chávez, R., Torres-Márquez, M. E. Moreno-Sánchez, R. 2001. The membrane-bound l- and d-lactate dehydrogenase activities in mitochondria from Euglena gracilis. Arch. Biochem. Biophys., 390 : 295 303.
20. Jasso-Chávez, R., García-Cano, I., Marín-Hernandez, A., Mendoza-Cozatl, D., Rendón, J. L. Moreno-Sánchez, R. 2005. The bacterial-like lactate shuttle components from heterotrophic Euglena gracilis. Biochim. Biophys. Acta, 1709 : 181 190.
21. Jornvall, H. 1994. The alcohol dehydrogenase system. In : Jannson, B. et al. (ed.), Towards a Molecular Basis of Alcohol Use and Abuse. Birkhause Verlag, Basel, Switzerland. p. 221 229.
22. Julistiono, H. Briand, J. 1992. Microsomal ethanol-oxidizing system in Euglena gracilis. Similarities between Euglena and mammalian cell systems. Comp. Biochem. Physiol. B, 102 : 747 755.
23. Mego, J. L. Farb, R. M. 1974. Alcohol dehydrogenases of Euglena gracilis, strain Z. Biochim. Biophys. Acta, 350 : 237 239.
24. Munir, I., Nakazawa, M., Harano, K., Yamaji, R., Inui, H., Miyatake, K. Nakano, Y. 2002. Occurrence of a novel NADP(+)-linked alcohol dehydrogenase in Euglena gracilis. Comp. Biochem. Physiol. B, 132 : 535 540.
25. Niefind, K., Müller, J., Riebel, B., Hummel, W. Schomburg, D. 2003. The crystal structure of R-specific alcohol dehydrogenase from Lactobacillus brevis suggests the structural basis of its metal dependence. J. Mol. Biol., 327 : 317 328.
26. Ono, K., Kawanaka, Y., Izumi, Y., Inui, H., Miyatake, K., Kitaoka, S. Nakano, Y. 1995. Mitochondrial alcohol dehydrogenase from ethanol-grown Euglena gracilis. J. Biochem., 117 : 1178 1182.
27. Perez-Miller, S. J. Hurley, T. D. 2003. Coenzyme isomerization is integral to catalysis in aldehyde dehydrogenase. Biochemistry, 42 : 7100 7109.
28. Reid, M. F. Fewson, C. A. 1994. Molecular characterization of microbial alcohol dehydrogenases. Crit. Rev. Microbiol., 20 : 13 56.
29. Riveros-Rosas, H., Julián-Sánchez, A., Villalobos-Molina, R., Pardo, J. P. Piña, E. 2003. Diversity, taxonomy and evolution of medum-chain dehydrogenase/reductase superfamily. Eur. J. Biochem., 270 : 3309 3334.
30. Rodríguez-Zavala, J. S., Ortiz-Cruz, M. A. Moreno-Sánchez, R. 2006. Characterization of an aldehyde dehydrogenase from Euglena gracilis. J. Eukaryot. Microbiol., 53 : 36 42.
31. Rodríguez-Zavala, J. S., García-García, J. D., Ortiz-Cruz, M. A. Moreno-Sánchez, R. 2007. Molecular mechanism of resistance to heavy metals in the protist Euglena gracilis. J. Environ. Sci. Health A, 42 : 1365 1378.
32. Schneider, T. Betz, A. 1985. Waxmonoester fermentation in Euglena gracilis T. Factors favouring the synthesis of odd-numbered fatty acids and alcohols. Planta. 166 : 67 73.
33. Schwede, T., Kopp, J., Guex, N. Peitsch, M. C. 2003. SWISS_MODEL: an automated protein homology-modeling server. Nucleic Acids Res., 31 : 3381 3385.
34. Sun, H. W. Plapp, B. V. 1992. Progressive sequence alignment and molecular evolution of the Zn-containing alcohol dehydrogenase family. J. Mol. Evol., 34 : 522 535.
35. Tokunaga, M., Nakano, Y. Kitaoka, S. 1979. Subcellular localization of the GABA-shunt enzymes in Euglena gracilis strain Z. J. Protozool., 26 : 471 473.
36. Watkins, R. F. Gray, M. W. 2006. The frequency of eubacterium-to- eukaryote lateral gene transfers shows significant cross-taxa variation within Amoebozoa. J. Mol. Evol., 63 : 801 814.
37. Wilson, B. W. Danforth, W. F. 1958. The extent of acetate and ethanol oxidation by Euglena gracilis. J. Gen. Microbiol., 18 : 535 542.