Detection of the nucleophosmin gene mutations in acute myelogenous leukemia through RT-PCR and polyacrylamide gel electrophoresis

European Journal of Haematology

Volumn 82, Issue 1, 2009, Pages 69-72

  Calvo, Karina Lucrecia ^a,b   Ojeda, Mara Jorgelina ^a   Ammatuna, Emanuele ^c   Lavorgna, Serena ^c   Ottone, Tiziana ^c   Targovnik, Hector Manuel ^b,d   Lo Coco, Francesco ^c   Noguera, Nélida Inés ^a,b  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

^c UNIVERSITY OF ROME TOR VERGATA   (Italy)

^d UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Acute myeloid leukemia; Normal karyotype; Nucleophosmin; RT PCR

Indexed keywords

NUCLEOPHOSMIN;

ACUTE GRANULOCYTIC LEUKEMIA; ADULT; ARTICLE; CARBOXY TERMINAL SEQUENCE; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; GENE MUTATION; HUMAN; HUMAN CELL; KARYOTYPE; MALE; MINIMAL RESIDUAL DISEASE; NPM 1 GENE; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TREATMENT RESPONSE;

BASE SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXONS; FEMALE; HUMANS; LEUKEMIA, MYELOID, ACUTE; MALE; MIDDLE AGED; MUTATION; NEOPLASM, RESIDUAL; NUCLEAR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 57749090248     PISSN: 09024441     EISSN: 16000609     Source Type: Journal    
DOI: 10.1111/j.1600-0609.2008.01155.x     Document Type: Article

References (15)

1. Cheson BD, Bennett JM, Kopecky KJ et al. International working group for diagnosis, standardization of response criteria, treatment outcomes, and reporting standards for the therapeutic trials in acute myeloid leukemia. Revised recommendations of the international working group for diagnosis, standardization of response criteria, treatment outcomes, and reporting standards for therapeutic trials in acute myeloid leukemia. J Clin Oncol 2003 15 : 4642 9.
2. Lo-Coco F, Breccia M, Noguera N, Miller WH Jr. Diagnostic value of detecting fusion proteins derived from chromosome translocations in acute leukaemia. Best Pract Res Clin Haematol 2003 16 : 653 70.
3. Falini B, Mecucci C, Tiacci E, et al. GIMEMA acute leukemia working party cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. New Engl J Med 2005 352 : 254 66.
4. Grisendi S, Mecucci C, Falini B, Pandolfi PP. Nucleophosmin and cancer. Nat Rev Cancer 2006 6 : 493 505.
5. Brady SN, Yu Y, Maggi LB Jr., Weber JD. ARF impedes NPM/B23 shuttling in an Mdm2-sensitive tumor suppressor pathway. Mol Cell Biol 2004 24 : 9327 38.
6. Okuda M. The role of nucleophosmin in centrosome duplication. Oncogene 2002 21 : 6170 4.
7. Naoe T, Suzuki T, Kiyoi H, Urano T. Nucleophosmin: a versatile molecule associated with hematological malignancies. Cancer Sci 2006 97 : 963 9.
8. Lim MJ, Wang XW. Nucleophosmin and human cancer. Cancer Detect Prev 2006 30 : 481 90.
9. Falini B, Bolli N, Shan J, et al. Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc + AML. Blood 2006 107 : 4514 23.
10. Thiede C, Koch S, Creutzig E, Steudel C, Illmer T, Schaich M, Ehninger G. Prevalence and prognostic impact of NPM1 mutations in 1485 adult patients with acute myeloid leukemia (AML). Blood 2006 107 : 4011 20.
11. Pasqualucci L, Liso A, Martelli MP, et al. Mutated nucleophosmin detects clonal multilineage involvement in acute myeloid leukemia: impact on WHO classification. Blood 2006 108 : 4146 55.
12. Chou WC, Tang JL, Wu SJ, Tsay W, Yao M, Huang SY, Huang KC, Chen CY, Huang CF, Tien HF. Clinical implications of minimal residual disease monitoring by quantitative polymerase chain reaction in acute myeloid leukemia patients bearing nucleophosmin (NPM1) mutations. Leukemia 2007 21 : 998 1004.
13. Noguera NI, Ammatuna E, Zangrilli D, Lavorgna S, Divona M, Buccisano F, Amadori S, Mecucci C, Falini B, Lo-Coco F. Simultaneous detection of NPM1 and FLT3-ITD mutations by capillary electrophoresis in acute myeloid leukemia. Leukemia 2005 19 : 1479 82.
14. Ammatuna E, Noguera NI, Zangrilli D, Curzi P, Panetta P, Bencivenga P, Amadori S, Federici G, Lo-Coco F. Rapid detection of nucleophosmin (NPM1) mutations in acute myeloid leukemia by denaturing HPLC. Clin Chem 2005 51 : 2165 7.
15. Chomczynsky P, Sacchi N. Single step method of RNA isolation by acid guanidium thiocyanate-phenol chloroform extraction. Anal Biochem 1987 162 : 156 9.