A new use for long-term frozen brain tissue: Golgi impregnation

Journal of Neuroscience Methods

Volumn 176, Issue 2, 2009, Pages 72-77

  Melendez Ferro, Miguel ^a   Perez Costas, Emma ^a   Roberts, Rosalinda C ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

Cortex; Dendritic spine; Neuroanatomy; Neuropathology; Schizophrenia; Striatum; Synapse

Indexed keywords

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN TISSUE; CONTROLLED STUDY; CORPUS STRIATUM; DENDRITIC SPINE; FREEZING; GOLGI STAIN; HUMAN; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL; RAT; STAINING;

ANIMALS; BRAIN; CRYOPRESERVATION; DENDRITIC SPINES; HUMANS; POSTMORTEM CHANGES; PYRAMIDAL CELLS; RATS; SILVER STAINING; TISSUE FIXATION;

EID: 57649157696     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.08.021     Document Type: Article

References (20)

1. Baloyannis S.J., Costa V., Mauroudis I., Psaroulis D., Manolides S.L., and Manolides L.S. Dendritic and spinal pathology in the acoustic cortex in Alzheimer's disease: morphological and morphometric estimation by Golgy technique and electron microscopy. Acta Otolaryngol 127 (2007) 351-354
2. Buell S.J. Golgi-Cox and rapid Golgi methods as applied to autopsied human brain tissue: widely disparate results. J Neuropathol Exp Neurol 41 (1982) 500-507
3. Colonnier M. The tangential organization of the visual cortex. J Anat 98 (1964) 327
4. Cox W. Impragnation des centralen Nervensystems mit Quecksilbersalzen. Arch Mikr Anat 98 (1891) 327
5. Fiala J.C., Spacek J., and Harris K.M. Dendritic spine pathology: cause or consequence of neurological disorders?. Brain Res Rev 39 (2002) 29-54
6. Garcia-Lopez P., Garcia-Martin V., and Freire M. Three-dimensional reconstruction and quantitative study of a pyramidal cell of a Cajal histological preparation. J Neurosci 26 (2006) 11249-11252
7. Gerfen C.R. Indirect-pathway neurons lose their spines in Parkinson disease. Nature Neurosci 9 (2006) 157-158
8. Glantz L.A., and Lewis D.A. Decreased dendritic spine density on prefrontal cortical pyramidal neurons in schizophrenia. Arch Gen Psychiatr 57 (2000) 65-73
9. Glaser E.M., and Van der Loos H. Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J Neurosci Meth 4 (1981) 117-125
10. Halpain S., Spencer K., and Graber S. Dynamics and pathology of dendritic spines. Prog Brain Res 147 (2005) 29-37
11. Hill J.J., Hashimoto T., and Lewis D.A. Molecular mechanisms contributing to dendritic spine alterations in the prefrontal cortex of subjects with schizophrenia. Mol Psychiatry 11 (2006) 557-566
12. Kelley J.J., Gao X.M., Tamminga C.A., and Roberts R.C. The effect of chronic haloperidol treatment in dendritic spines in the rat striatum. Exp Neurol 146 (1997) 471-478
13. Kopsch F. Erfahrungen uber die Verwendung des Formaldehyds bei der Chromsilber-Impragnation. Anat Anz 11 (1896) 727
14. Melendez-Ferro M, Perez-Costas E, Roberts RC. Applying Golgi techniques to long-term stored human brain tissue. Program No. 489.13. 2006 Neuroscience Meeting Planner. Atlanta, GA: Society for Neuroscience, 2006. Online.
15. Perez-Costas E., Melendez-Ferro M., and Roberts R.C. In: Mendez-Vilas A., and Diaz J. (Eds). Microscopy techniques and the study of synapses. Modern research and educational topics in microscopy vol. 1 (2007), Badajoz, Formatex 164-170
16. Ramon y Cajal S. Estructura de los centros nerviosos de las aves. Rev Trim Histol Norm Patol 1 (1888) 1-10
17. Ramon y Cajal S., and De Castro F. Elementos de tecnica micrografica del sistema nervioso. Tipografia artistica, Madrid 1972. 2nd ed. (1933), Salvat Editores S A., Barcelona p. 283
18. Rosoklija G., Mancevski B., Ilievski B., Perera T., Lisanby S.H., Coplan J.D., et al. Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys. J Neurosci Meth 131 (2003) 1-7
19. Spires T.L., Grote H.E., Garry S., Cordery P.M., Van Dellen A., Blakemore C., et al. Dendritic spine pathology and deficits in experience-dependent dendritic plasticity in R6/1 Huntington's disease transgenic mice. Eur J Neurosci 19 (2004) 2799-2807
20. Zhang H., Weng S.J., and Hutsler J.J. Does microwave enhance the Golgi methods? A quantitative analysis of disparate staining patterns in the cerebral cortex. J Neurosci Meth 124 (2003) 145-155