Membrane-based yeast two-hybrid system to detect protein interactions

Current Protocols in Protein Science

Volumn , Issue SUPPL. 52, 2008, Pages

  Lentze, Nicolas ^a   Auerbach, Daniel ^a  

^a DUALSYSTEMS BIOTECH AG   (Switzerland)

Author keywords

Membrane proteins; Membrane based yeast two hybrid system; Protein interactions

Indexed keywords

COMPLEMENTARY DNA; EPIDERMAL GROWTH FACTOR RECEPTOR; FUNGAL PROTEIN; HYBRID PROTEIN; INTEGRAL MEMBRANE PROTEIN; MEMBRANE PROTEIN; NUCLEAR PROTEIN; PLASMID BT3 PROTEIN STE2 FUSION PROTEIN; PLASMID BT3 PROTEIN SUC2 FUSION PROTEIN; PLASMID VECTOR; PROTEIN SFII; PROTEIN STE2; PROTEIN SUC2; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR LEXA VP16; TYROSINE KINASE RECEPTOR; UNCLASSIFIED DRUG; VASCULOTROPIN RECEPTOR;

CONTROLLED STUDY; FUNGAL STRAIN; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN MODIFICATION; REPORTER GENE; REVIEW; TERMINAL SEQUENCE; TWO HYBRID SYSTEM; YEAST; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; FUNGAL CELL; MEMBRANE BASED YEAST TWO HYBRID SYSTEM; PLASMID; PRACTICE GUIDELINE; PROCESS DESIGN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SEQUENCE ANALYSIS;

EID: 57549099663     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1917s52     Document Type: Review

References (7)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.) 2008. Current Protocols in Molecular Biology, Chapters 1 and 2. John Wiley & Sons, Hoboken, N.J.
2. Fashena, S.J., Serebriiskii, I., and Golemis, E.A. 2000. The continued evolution of two-hybrid screening approaches in yeast: How to outwit different preys with different baits. Gene 250:1-14.
3. Fields, S. and Song, O. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
4. Johnsson, N. and Varshavsky, A. 1994. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. U.S.A. 91:10340-10344.
5. Mockli, N., Deplazes, A., Hassa, P.O., Zhang, Z., Peter, M., Hottiger, M.O., Stagljar, I., and Auerbach, D. 2007. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques 42:725-730.
6. Stagljar, I., Korostensky, C., Johnsson, N., and te Heesen, S. 1998. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc. Natl. Acad. Sci. U.S.A. 95:5187-5192.
7. Thaminy, S., Auerbach, D., Arnoldo, A., and Stagljar, I. 2003. Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res. 13:1744-1753.