Purification and biochemical characterization of a membrane-bound [NiFe]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium, Hydrogenophaga sp. AH-24

FEMS Microbiology Letters

Volumn 290, Issue 1, 2009, Pages 114-120

  Yoon, Ki Seok ^a   Sakai, Yukiko ^a   Tsukada, Natsuki ^a   Fujisawa, Kiyoshi ^b   Nishihara, Hirofumi ^a  

^a IBARAKI UNIVERSITY   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Electron paramagnetic resonance; Hydrogen oxidizing bacterium; Hydrogenophaga sp. AH 24; Kinetic constants; Membrane bound NiFe hydrogenase; Purification

Indexed keywords

HYDROGEN; HYDROGENASE; MEMBRANE ENZYME; METHYLENE BLUE; PROTEIN SUBUNIT;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; GRAM NEGATIVE BACTERIUM; HYDROGENOPHAGA; MEMBRANE BINDING; MOLECULAR WEIGHT; NONHUMAN; OXIDATION; PRIORITY JOURNAL; THERMOSTABILITY;

AMINO ACID SEQUENCE; AUTOTROPHIC PROCESSES; CELL MEMBRANE; COMAMONADACEAE; ELECTRON SPIN RESONANCE SPECTROSCOPY; HYDROGEN; HYDROGENASE; KINETICS; OXIDATION-REDUCTION; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); HYDROGENOPHAGA;

EID: 57449119095     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2008.01417.x     Document Type: Article

References (27)

1. Adams MW Hall DO (1979) Properties of the solubilized membrane-bound hydrogenase from the photosynthetic bacterium Rhodospirillum rubrum. Arch Biochem Biophys 195 : 288 299.
2. Adams MW Mortenson LE (1984) The physical and catalytic properties of hydrogenase II of Clostridium pasteurianum. A comparison with hydrogenase I. J Biol Chem 259 : 7045 7055.
3. Albracht SP (1994) Nickel hydrogenases: in search of the active site. Biochim Biophys Acta 1188 : 167 204.
4. Bernhard M, Benelli B, Hochkoeppler A, Zannoni D Friedrich B (1997) Functional and structural role of the cytochrome b subunit of the membrane-bound hydrogenase complex of Alcaligenes eutrophus H16. Eur J Biochem 248 : 179 186.
5. 2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase. J Biol Chem 276 : 15592 15597.
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 : 248 254.
7. Brugna-Guiral M, Tron P, Nitschke W, Stetter KO, Burlat B, Guigliarelli B, Bruschi M Giudici-Orticoni MT (2003) [NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics. Extremophiles 7 : 145 157.
8. 2-sensing [NiFe] hydrogenase from Ralstonia eutropha H16 is based on limited access of oxygen to the active site. J Biol Chem 280 : 23791 23796.
9. +-reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 consists of six subunits and can be specifically activated by NADPH. J Bacteriol 187 : 3122 3132.
10. Fontecilla-Camps JC, Volbeda A, Cavazza C Nicolet Y (2007) Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases. Chem Rev 107 : 4273 4303.
11. Guiral M, Tron P, Belle V, Aubert C, Léger C, Guigliarelli B Giudici-Orticoni MT (2006) Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: physicochemical properties. Int J Hydrogen Energ 31 : 1424 1431.
12. Knüttel K, Schneider K, Schlegel HG Müller A (1989) The membrane-bound hydrogenase from Paracoccus denitrificans: purification and molecular characterization. Eur J Biochem 179 : 101 108.
13. Lubitz W, Reijerse E van Gastel M (2007) [NiFe] and [FeFe] hydrogenases studied by advanced magnetic resonance techniques. Chem Rev 107 : 4331 4365.
14. Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262 : 10035 10038.
15. Mertens R Liese A (2004) Biotechnological applications of hydrogenases. Curr Opin Biotech 15 : 343 348.
16. Nishihara H, Igarashi Y Kodama T (1991) Growth characteristics and high cell-density cultivation of a marine obligately chemolithoautotrophic hydrogen-oxidizing bacterium Hydrogenovibrio marinus strain MH-110 under a continuous gas-flow system. J Ferment Bioeng 72 : 358 361.
17. Nishihara H, Miyashita Y, Aoyama K, Kodama T, Igarashi Y Takamura Y (1997) Characterization of an extremely thermophilic and oxygen-stable membrane-bound hydrogenase from a marine hydrogen-oxidizing bacterium Hydrogenovibrio marinus. Biochem Bioph Res Co 232 : 766 770.
18. Ono T, Ishii M, Yoon K-S, Igarashi Y Kodama T (1995) Purification and characterization of membrane-bound hydrogenase from a thermophilic hydrogen-oxidizing bacterium, Pseudomonas hydrogenothermophila strain TH-1. Biosci Biotech Bioch 59 : 917 919.
19. Schneider K, Patil DS Cammack R (1983) ESR properties of membrane-bound hydrogenases from aerobic hydrogen bacteria. Biochim Biophys Acta 748 : 353 361.
20. 2 activation. Chem Rec 7 : 37 46.
21. Teixeira M, Moura I, Xavier AV, Huynh BH, DerVartanian DV, Peck HD Jr., LeGall J Moura JJ (1985) Electron paramagnetic resonance studies of the mechanism of activation and the catalytic cycle of the nickel-containing hydrogenase from Desulfovibrio gigas. J Biol Chem 260 : 8942 8950.
22. Teixeira M, Fauque G, Moura I, Lespinat PA, Berlier Y, Prickril B, Peck HD Jr., Xavier AV, Le Gall J Moura JJ (1987) Nickel-[iron-sulfur]-selenium- containing hydrogenases from Desulfovibrio baculatus (DSM 1743). Redox centers and catalytic properties. Eur J Biochem 167 : 47 58.
23. Valente FM, Oliveira AS, Gnadt N, Pacheco I, Coelho AV, Xavier AV, Teixeira M, Soares CM Pereira IA (2005) Hydrogenases in Desulfovibrio vulgaris hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase. J Biol Inorg Chem 10 : 667 682.
24. Vignais PM Billoud B (2007) Occurrence, classification, and biological function of hydrogenases: an overview. Chem Rev 107 : 4206 4272.
25. Volbeda A, Charon M, Piras C, Hatchikian EC, Frey M Fontecilla-Campus JC (1995) Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas. Nature 373 : 580 587.
26. Yoon K-S, Bobst C, Hemann CF, Hille R Tabita FR (2001) Spectroscopic and functional properties of novel 2[4Fe-4S] cluster-containing ferredoxins from the green sulfur bacterium Chlorobium tepidum. J Biol Chem 276 : 44027 44036.
27. Yoon K-S, Tsukada N, Sakai Y, Ishii M, Igarashi Y Nishihara H (2008) Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24. FEMS Microbiol Lett 278 : 94 100.