Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe

European Journal of Plant Pathology

Volumn 123, Issue 1, 2009, Pages 117-123

  Aparicio, Frederic ^a   Soler, Salvador ^b   Aramburu, José ^c   Galipienso, Luis ^c   Nuez, Fernando ^b   Pallás, Vicente ^a   López, Carmelo ^b  

^a Universitat Politècnica de València   (Spain)

^b UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

^c IRTA   (Spain)

Author keywords

Diagnosis; Dig RNA polyprobe; Tissue printing; Tomato

Indexed keywords

DETECTION METHOD; ECONOMIC IMPACT; FRUIT; ISOTOPIC ANALYSIS; MOLECULAR ANALYSIS; RNA; SENSITIVITY ANALYSIS; STEROID; VIRAL DISEASE; WILT;

CUCUMBER MOSAIC VIRUS; LYCOPERSICON ESCULENTUM; PARIETARIA; PARIETARIA MOTTLE VIRUS; PEPINO MOSAIC VIRUS; SOLANUM TUBEROSUM; TOMATO MOSAIC VIRUS; TOMATO SPOTTED WILT VIRUS; TOSPOVIRUS;

EID: 57449109387     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-008-9347-5     Document Type: Article

References (19)

1. J. Aramburu L. Galipienso M. Matas 2006 Characterization of Potato virus Y isolates from tomato crops in northeast Spain European Journal of Plant Pathology 115 247 258
2. M. C. Cañizares F. Aparicio K. Amari V. Pallas 2001 Studies on the aetiology of the apricot "viruela" disease Acta Horticulturae 90 1330 1336
3. O. Cohen O. Batuman G. Stanbekova T. Sano M. Mawassi M. Bar-Joseph 2006 Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees Virus Genes 33 287 292
4. M. C. Córdoba-Sellés A. García-Rández A. Alfaro-Fernández C. Jordá 2007 Seed transmission of Pepino mosaic virus and efficacy of tomato seed disinfection treatments Plant Disease 91 1250 1254
5. L. Galipienso M. C. Herranz V. Pallas J. Aramburu 2005 Detection of a tomato strain of Parietaria mottle virus (PMoV-T) by molecular hybridization and RT-PCR in field samples from north-eastern Spain Plant Pathology 54 29 35
6. D. Gallitelli G. P. Martelli K. G. Selassie G. Marchoux 1995 Progress in the biological and molecular studies of some important viruses of Solanaceae in the Mediterranean Acta Horticulturae 412 503 514
7. M. C. Herranz J. A. Sanchez-Navarro F. Aparicio V. Pallás 2005 Simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or 'polyprobe'. Journal of Virological Methods 124 49 55
8. P. Ivars M. Alonso M. Borja C. Hernandez 2004 Development of a non-radioactive dot-blot hybridisation assay for the detection of Pelargonium flower break virus and Pelargonium line pattern virus European Journal of Plant Patholology 110 275 283
9. D. James A. Varga V. Pallas T. Candresse 2006 Strategies for simultaneous detection of multiple plant viruses Canadian Journal of Plant Pathology 28 16 29
10. B. Mandic M. Al Rwahnih A. Myrta G. Gomez V. Pallás 2007 Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia European Journal of Plant Pathology 120 167 176
11. P. Más V. Pallás 1995 Non-isotopic tissue-printing hybridization: a new technique to study long-distance plant virus movement Journal of Virological Methods 52 317 326
12. P. Más J. A. Sanchez-Navarro M. A. Sanchez-Pina V. Pallás 1993 Chemiluminescent and colorigenic detection of Cherry leaf roll virus with digoxigenin-labelled RNA probes Journal of Virological Methods 45 93 102
13. V. Pallás P. Más J. A. Sanchez-Navarro 1998 Detection of plant RNA viruses by nonisotopic dot-blot hybridization Methods in Molecular Biology (Clifton, N.J.) 81 461 468
14. E. Ramasso P. Roggero G. Dellavalle V. Lisa 1997 Necrosi apicale del pomodoro causata da un ilarvirus Informatore Fitopatologico 1 71 77
15. J. Romero-Durbán M. Cambra N. Durán-Vila 1995 A simple imprint-hybridization method for detection of viroids Journal of Virological Methods 55 37 47
16. M. Saade F. Aparicio J. A. Sanchez-Navarro M. C. Herranz A. Myrta B. Di-Terlizzi 2000 Simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridisation and multiplex reverse-transcription polymerase chain reaction Phytopathology 90 1330 1336
17. P. Saldarelli L. Barbarossa F. Grieco D. Gallitelli 1996 Digoxigenin-labelled riboprobes applied to phytosanitary certification of tomato in Italy Plant Disease 80 1343 1346
18. J. A. Sanchez-Navarro M. C. Cañizares E. A. Cano V. Pallás 1999 Simultaneous detection of five carnation viruses by non-isotopic molecular hybridization Journal of Virological Methods 82 167 175
19. J. R. Thompson S. Wetzel M. M. Klerks D. Vaskova C. D. Schoen J. Spak 2003 Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control Journal of Virological Methods 111 85 93