Preferential utilization of NADPH as the endogenous electron donor for NAD(P)H:quinone oxidoreductase 1 (NQO1) in intact pulmonary arterial endothelial cells

Free Radical Biology and Medicine

Volumn 46, Issue 1, 2009, Pages 25-32

  Bongard, Robert D ^a   Lindemer, Brian J ^a   Krenz, Gary S ^b   Merker, Marilyn P ^a,c  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b MARQUETTE UNIVERSITY   (United States)

^c CLEMENT J ZABLOCKI VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

High performance liquid chromatography; Lung; NAD(P)H:quinone oxidoreductase; Phase II enzymes; Pulmonary endothelial cells; Pyridine nucleotides; Quinone

Indexed keywords

DEOXYGLUCOSE; DUROQUINONE; EPIANDROSTERONE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; IODOACETIC ACID; LACTIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PENTOSE PHOSPHATE; PYRIDINE NUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME ACTIVITY; GLYCOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LUNG ENDOTHELIUM; NONHUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL;

ANDROSTERONE; ANIMALS; CATTLE; CELLS, CULTURED; CYTOSOL; DEOXYGLUCOSE; ENDOTHELIAL CELLS; GLYCOLYSIS; IODOACETATES; LACTIC ACID; NAD(P)H DEHYDROGENASE (QUINONE); NADP; OXIDATION-REDUCTION; PULMONARY ARTERY; SUBSTRATE SPECIFICITY;

EID: 57449088630     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2008.09.007     Document Type: Article

References (47)

1. Lind C., Cadenas E., Hochstein P., and Ernster L. DT-diaphorase: purification, properties, and function. Methods Enzymol. 186 (1990) 287-301
2. Cadenas E. Antioxidant and prooxidant functions of DT-diaphorase in quinone metabolism [review]. Biochem. Pharmacol. 49 (1995) 127-140
3. Ernster L., Danielson L., and Ljunggren M. DT diaphorase. I. Purification from the soluble fraction of rat-liver cytoplasm, and properties. Biochim. Biophys. Acta 58 (1962) 171-188
4. Merker M.P., Audi S.H., Bongard R.D., Lindemer B.J., and Krenz G.S. Influence of pulmonary arterial endothelial cells on quinone redox status: effect of hyperoxia induced increase in NAD(P)H quinone oxidoreductase 1 (NQO1). Am. J. Physiol., Lung Cell. Mol. Physiol. 289 (2006) 788-797
5. Audi S.H., Bongard R.D., Krenz G.S., Rickaby D.A., Haworth S.T., Eisenhauer J., Roerig D.L., and Merker M.P. Effect of chronic hyperoxic exposure on duroquinone reduction in adult rat lungs. Am. J. Physiol., Lung Cell. Mol. Physiol. 289 (2005) L788-L797
6. Zhu H., Jia Z., Mahaney J.E., Ross D., Misra H.P., Trush M.A., and Li Y. The highly expressed and inducible endogenous NAD(P)H:quinone oxidoreductase 1 in cardiovascular cells acts as a potential superoxide scavenger. Cardiovasc. Toxicol. 7 (2007) 202-211
7. Ross D. Quinone reductases multitasking in the metabolic world. Drug Metab. Rev. 36 (2004) 639-654
8. Siegel D., Franklin W.A., and Ross D. Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors. Clin. Cancer Res. 4 (1998) 2065-2070
9. Audi S.H., Bongard R.D., Dawson C.A., Siegel D., Roerig D.L., and Merker M.P. Duroquinone reduction during passage through the pulmonary circulation. Am. J. Physiol., Lung Cell. Mol. Physiol. 285 (2003) L1116-L1131
10. Siegel D., and Ross D. Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues. Free Radic. Biol. Med. 29 (2000) 246-253
11. Ross D., Kepa J.K., Winski S.L., Beall H.D., Anwar A., and Siegel D. NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphisms. Chem. Biol. Interact. 129 (2000) 77-97
12. Siegel D., Gustafson D.L., Dehn D.L., Han J.Y., Boonchoong P., Berliner L.J., and Ross D. NAD(P)H:quinone oxidoreductase 1: role as a superoxide scavenger. Mol. Pharmacol. 65 (2004) 1238-1247
13. Iskander K., Gaikwad A., Paquet M., Long D.J., Brayton C., Barrios R., and Jaiswal A.K. Lower induction of p53 and decreased apoptosis in NQO1-null mice lead to increased sensitivity to chemical-induced skin carcinogenesis. Cancer Res. 65 (2005) 2054-2058
14. Gaikwad A., Long D.J., Stringer J.L., and Jaiswal A.K. In vivo role of NAD(P)H:quinone oxidoreductase 1 (NQO1) in the regulation of intracellular redox state and accumulation of abdominal adipose tissue. J. Biol. Chem. 276 (2001) 22559-22564
15. Palming J., Sjoholm K., Jernas M., Lystig T.C., Gummesson A., Romeo S., Lonn L., Lonn M., Carlsson B., and Carlsson L.M.S. The expression of NAD(P)H:quinone oxidoreductase 1 is high in human adipose tissue, reduced by weight loss, and correlates with adiposity, insulin sensitivity, and markers of liver dysfunction. J. Clin. Endocrinol. Metab. 92 (2007) 2346-2352
16. Seow H.A., Penketh P.G., Belcourt M.F., Tomasz M., Rockwell S., and Sartorelli A.C. Nuclear overexpression of NAD(P)H:quinone oxidoreductase 1 in Chinese hamster ovary cells increases the cytotoxicity of mitomycin C under aerobic and hypoxic conditions. J. Biol. Chem. 279 (2004) 31606-31612
17. Bello R.I., Kagan V.E., Tyurin V., Navarro F., Alcain F.J., and Villalba J.M. Regeneration of lipophilic antioxidants by NAD(P)H:quinone oxidoreductase 1. Protoplasma 221 (2003) 129-135
18. Ahn K., Sethi G., Jain A., Jaiswal A., and Aggarwal B. Genetic deletion of NAD(P)H:quinone oxidoreductase 1 abrogates activation of nuclear factor-KB, IKBα kinase, c-Jun N-terminal kinase, Akt, p38, and p44/42 mitogen-activated protein kinases and potentiates apoptosis. J. Biol. Chem. 281 (2006) 19798-19808
19. Dehn D.L., Siegel D., Zafar K.S., Reigan P., Swann E., Moody C.J., and Ross D. 5-Methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione, a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1, exhibits activity against human pancreatic cancer in vitro and in vivo. Mol. Cancer Ther. 5 (2006) 1702-1709
20. 0. Free Radic. Biol. Med. 34 (2003) 892-907
21. Audi S.H., Bongard R.D., Okamoto Y., Merker M.P., Roerig D.L., and Dawson C.A. Pulmonary reduction of an intravascular redox polymer. Am. J. Physiol., Lung Cell. Mol. Physiol. 280 (2001) L1290-L1299
22. Audi S.H., Olson L.E., Bongard R.D., Roerig D.L., Schulte M.L., and Dawson C.A. Toluidine blue O and methylene blue as endothelial redox probes in the intact lung. Am. J. Physiol., Heart Circ. Physiol. 278 (2000) H137-H150
23. Bongard R.D., Merker M.P., Shundo R., Okamoto Y., Roerig D.L., Linehan J.H., and Dawson C.A. Reduction of thiazine dyes by bovine pulmonary arterial endothelial cells in culture. Am. J. Physiol., Lung Cell. Mol. Physiol. 269 (1995) L78-L84
24. Merker M.P., Bongard R.D., Krenz G.S., Zhao H., Fernandes V., Kalyanaraman B., Hogg N., and Audi S.H. Impact of pulmonary arterial endothelial cells on duroquinone redox status. Free Radic. Biol. Med. 37 (2004) 86-103
25. Merker M.P., Bongard R.D., Kettenhofen N.J., Okamoto Y., and Dawson C.A. Intracellular redox status affects transplasma membrane electron transport in pulmonary arterial endothelial cells. Am. J. Physiol., Lung Cell. Mol. Physiol. 282 (2002) L36-L43
26. Merker M.P., Bongard R.D., Linehan J.H., Okamoto Y., Vyprachticky D., Brantmeier B.M., Roerig D.L., and Dawson C.A. Pulmonary endothelial thiazine uptake: separation of cell surface reduction from intracellular reoxidation. Am. J. Physiol., Lung Cell. Mol. Physiol. 272 (1997) L673-L680
27. Hartwick A.T., and Sivak J.G. Epithelial activity of hexokinase and glucose-6-phosphate dehydrogenase in cultured bovine lenses recovering from pharmaceutical-induced optical damage. Mol. Vis. 9 (2003) 594-600
28. Wick A.N., Drury D.R., Nakada H.I., and Wolfe J.B. Localization of the primary metabolic block produced by 2-deoxyglucose. J. Biol. Chem. 224 (1957) 963-969
29. Ozer N., Bilgi C., and Hamdi Ogus I. Dog liver glucose-6-phosphate dehydrogenase: purification and kinetic properties. Int. J. Biochem. Cell Biol. 34 (2002) 253-262
30. Ozer N., Aksoy Y., and Ogus I.H. Kinetic properties of human placental glucose-6-phosphate dehydrogenase. Int. J. Biochem. Cell Biol. 33 (2001) 221-226
31. Harris R.A. Carbohydrate metabolism: I. Major metabolic pathways and their control. In: Devlin T.A. (Ed). Textbook of Biochemistry with Clinical Correlations (1997), Wiley-Liss, New York 267-333
32. + depletion and cell death induced by oxidative stress. J. Biol. Chem. 278 (2003) 18426-18433
33. Michan S., and Sinclair D. Sirtuins in mammals: insights into their biological function. Biochem. J. 404 (2007) 1-13
34. Gupte S.A., Okada T., McMurtry I.F., and Oka M. Role of pentose phosphate pathway-derived NADPH in hypoxic pulmonary vasoconstriction. Pulm. Pharmacol. Ther. 19 (2006) 303-309
35. Gordon G., Mackow M.C., and Levy H.R. On the mechanism of interaction of steroids with human glucose 6-phosphate dehydrogenase. Arch. Biochem. Biophys. 318 (1995) 25-29
36. Williamson D.H., Lund P., and Krebs H.A. The redox state of free nucleotide-adenine nucleotide in the cytoplasm and mitochondria of rat liver. Biochem. J. 103 (1967) 514-527
37. Sies H. Nicotinamide nucleotide compartmentation. In: Sies H. (Ed). Metabolic Compartmentation (1982), Academic Press, London 205-231
38. Veech R.L., Eggleston L.V., and Krebs H.A. The redox state of free nicotinamide-adenine dinucleotide phosphate in the cytoplasm of rat liver. Biochem. J. 115 (1969) 609-619
39. Gupte S.A., and Wolin M.S. Hypoxia promotes relaxation of bovine coronary arteries through lowering cytosolic NADPH. Am. J. Physiol., Heart Circ. Physiol. 290 (2006) H2228-H2238
40. Siraki A.G., Chan T.S., and O'Brien P.J. Application of quantitative structure-toxicity relationships for the comparison of the cytotoxicity of 14 p-benzoquinone congeners in primary cultured rat hepatocytes versus PC12 cells. Toxicol. Sci. 81 (2004) 148-159
41. Dehn D.L., Inayat-Hussain S.H., and Ross D. RH1 induces cellular damage in an NAD(P)H:quinone oxidoreductase 1-dependent manner: relationship between DNA cross-linking, cell cycle perturbations, and apoptosis. J Pharmacol. Exp. Ther. 313 (2005) 771-779
42. Danson S., Ward T.H., Butler J., and Ranson M. DT-diaphorase: a target for new anticancer drugs. Cancer Treat. Rev. 30 (2004) 437-449
43. Chan T.S., Teng S., Wilson J.X., Galati G., Khan S., and O'Brien P.J. Coenzyme Q cytoprotective mechanisms for mitochondrial complex I cytopathies involves NAD(P)H:quinone oxidoreductase 1(NQO1). Free Radic. Res. 36 (2002) 421-427
44. Krebs H.A., and Veech R.L. Equilibrium relations between pyridine nucleotides and adenine nucleotides and their roles in the regulation of metabolic processes. Adv. Enzyme Regul. 7 (1969) 397-412
45. Eggleston L.V., and Krebs H.A. Regulation of the pentose phosphate cycle. Biochem. J. 138 (1974) 425-435
46. Bucher T., Brauser B., Conze A., Klein F., Langguth O., and Sies H. State of oxidation-reduction and state of binding in the cytosolic NADH-system as disclosed by equilibration with extracellular lactate-pyruvate in hemoglobin-free perfused rat liver. Eur. J. Biochem. 27 (1972) 301-317
47. Conover T.E., and Ernster L. DT diaphorase. IV. Coupling of extramitochondrial reduced pyridine nucleotide oxidation to mitochondrial respiratory chain. Biochim. Biophys. Acta 67 (1963) 268-280