Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity

American Journal of Physiology - Renal Physiology

Volumn 295, Issue 5, 2008, Pages

  Blessing, Nathan W ^a   Blount, Mitsi A ^a   Sands, Jeff M ^a   Martin, Christopher F ^a   Klein, Janet D ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Concentrating mechanism; Osmolality; Renal; Trafficking

Indexed keywords

CARRIER PROTEIN; HYPERTONIC SOLUTION; MEMBRANE PROTEIN; SODIUM CHLORIDE; SUCROSE; UNCLASSIFIED DRUG; UREA; UREA TRANSPORTER A1; UREA TRANSPORTER A3;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOTINYLATION; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; HYPEROSMOLARITY; KIDNEY CELL; KIDNEY MEDULLA; MALE; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; RAT; STIMULATION; SUSPENSION; TRANSPORT KINETICS; WESTERN BLOTTING;

EID: 57349177906     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.90228.2008     Document Type: Article

References (22)

1. Blount MA, Klein JD, Martin CF, Tchapyjnikov D, Sands JM. Forskolin stimulates phosphorylation and membrane accumulation of UT-A3. Am J Physiol Renal Physiol 293: F1308-F1313, 2007.
2. Chou CL, Knepper MA. Inhibition of urea transport in inner medullary collecting duct by phloretin and urea analogues. Am J Physiol Renal Fluid Electrolyte Physiol 257: F359-F365, 1989.
3. Chou CL, Sands JM, Nonoguchi H, Knepper MA. Concentration dependence of urea and thiourea transport in rat inner medullary collecting duct. Am J Physiol Renal Fluid Electrolyte Physiol 258: F486-F494, 1990.
4. Fröhlich O, Klein JD, Smith PM, Sands JM, Gunn RB. Regulation of UT-A1-mediated transepithelial urea flux in MDCK cells. Am J Physiol Cell Physiol 291: C600-C606, 2006.
5. Gillin AG, Sands JM. Characteristics of osmolarity-stimulated urea transport in rat IMCD. Am J Physiol Renal Fluid Electrolyte Physiol 262: F1061-F1067, 1992.
6. Gillin AG, Star RA, Sands JM. Osmolarity-stimulated urea transport in rat terminal IMCD: role of intracellular calcium. Am J Physiol Renal Fluid Electrolyte Physiol 265: F272-F277, 1993.
7. Karakashian A, Timmer RT, Klein JD, Gunn RB, Sands JM, Bagnasco SM. Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4. J Am Soc Nephrol 10: 230-237, 1999.
8. Klein JD, Fröhlich O, Blount MA, Martin CF, Smith TD, Sands JM. Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts. J Am Soc Nephrol 17: 2680-2686, 2006.
9. Klein JD, Price SR, Bailey JL, Jacobs JD, Sands JM. Glucocorticoids mediate a decrease in AVP-regulated urea transporter in diabetic rat inner medulla. Am J Physiol Renal Physiol 273: F949-F953, 1997.
10. Kokko JP, Rector FC Jr. Countercurrent multiplication system without active transport in inner medulla. Kidney Int 2: 214-223, 1972.
11. Nielsen S, Knepper MA. Vasopressin activates collecting duct urea transporters and water channels by distinct physical processes. Am J Physiol Renal Fluid Electrolyte Physiol 265: F204-F213, 1993.
12. Sands JM, Layton HE. The urine concentrating mechanism and urea transporters. In: The Kidney: Physiology and Pathophysiology (4th ed.), edited by Alpern RJ and SC H. San Diego: Elsevier, 2008, p. 1143-1178.
13. 2O transport in inner medullary collecting duct subsegments. Am J Physiol Renal Fluid Electrolyte Physiol 253: F823-F832, 1987.
14. Sands JM, Schrader DC. An independent effect of osmolality on urea transport in rat terminal inner medullary collecting ducts. J Clin Invest 88: 137-142, 1991.
15. Sands JM, Timmer RT, Gunn RB. Urea transporters in kidney and erythrocytes. Am J Physiol Renal Physiol 273: F321-F339, 1997.
16. Seamon KB, Padgett W, Daly JW. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci USA 78: 3363-3367, 1981.
17. Smith CP, Potter EA, Fenton RA, Stewart GS. Characterization of a human colonic cDNA encoding a structurally novel urea transporter, hUT-A6. Am J Physiol Cell Physiol 287: C1087-C1093, 2004.
18. Star RA, Nonoguchi H, Balaban R, Knepper MA. Calcium and cyclic adenosine monophosphate as second messengers for vasopressin in the rat inner medullary collecting duct. J Clin Invest 81: 1879-1888, 1988.
19. Stephenson JL. Concentration of urine in a central core model of the renal counterflow system. Kidney Int 2: 85-94, 1972.
20. Stewart GS, Fenton RA, Wang W, Kwon TH, White SJ, Collins VM, Cooper G, Nielsen S, Smith CP. The basolateral expression of mUT-A3 in the mouse kidney. Am J Physiol Renal Physiol 286: F979-F987, 2004.
21. Terris JM, Knepper MA, Wade JB. UT-A3: localization and characterization of an additional urea transporter isoform in the IMCD. Am J Physiol Renal Physiol 280: F325-F332, 2001.
22. Zhang C, Sands JM, Klein JD. Vasopressin rapidly increases phosphorylation of UT-A1 urea transporter in rat IMCDs through PKA. Am J Physiol Renal Physiol 282: F85-F90, 2002.