Current progress of immunostains in Mohs micrographic surgery: A review

Dermatologic Surgery

Volumn 34, Issue 12, 2008, Pages 1621-1636

  Thosani, Maya K ^a   Marghoob, Ashfaq ^b   Chen, Chih Shan Jason ^b  

^a STONY BROOK UNIVERSITY   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BASAL CELL CARCINOMA; CHEMOSURGERY; DERMATOFIBROSARCOMA PROTUBERANS; DIAGNOSTIC KIT; FIBROXANTHOMA; HUMAN; IMMUNOASSAY; IMMUNOHISTOCHEMISTRY; MELANOMA; MICROCYSTIC ADNEXAL CARCINOMA; PAGET SKIN DISEASE; PRIORITY JOURNAL; REVIEW; SARCOMA; SEBACEOUS CARCINOMA; SKIN CANCER; SQUAMOUS CELL CARCINOMA; STAIN; SWEAT GLAND CARCINOMA;

HUMANS; IMMUNOHISTOCHEMISTRY; MOHS SURGERY; SKIN NEOPLASMS; STAINING AND LABELING;

EID: 57249083955     PISSN: 10760512     EISSN: 15244725     Source Type: Journal    
DOI: 10.1111/j.1524-4725.2008.34339.x     Document Type: Review

References (127)

1. Albertini JG, Elston DM, Libow LF, et al. Mohs micrographic surgery for melanoma: a case series, a comparative study of immunostains, and informative case report, and a unique mapping technique. Dermatol Surg 2002 28 : 656 65.
2. Mondragon RM, Barrett TL. Current concepts: the use of immunoperoxidase techniques in Mohs micrographic surgery. J Am Acad Dermatol 2000 43 : 66 71.
3. Zalla MJ, Lim KK, DiCaudo DJ, et al. Mohs micrographic excision of melanoma using immunostains. Dermtol Surg 2000 26 : 771 4.
4. Jimenez FJ, Grichnik JM, Buchanan MD, et al. Immunohistochemical techniques in mohs micrographic surgery: their potential use in the detection of neoplastic cells masked by inflammation. J Am Acad Dermatol 1995 32 : 89 94.
5. Harlow E, Lane D. Using antibodies. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press 1999.
6. Robinson JK. Current histologic preparation methods for Mohs Micrographic Surgery. Dermatol Surg 2001 27 : 555 60.
7. Commentary by Zalla MJ in : Robinson JK. Current histologic preparation methods for mohs micrographic surgery. Dermatol Surg 2001 27 : 555 60.
8. Griego RD, Zitelli JA. Mohs micrographic surgery using HMB-45 for a recurrent acral melanoma. Dermatol Surg 1998 24 : 1003 6.
9. Blessing K, Sanders DSA, Grant JJH. Comparison of immunohistochemical staining of the novel antibody Melan-A with S-100 protein and HMB-45 in malignant melanoma and melanoma variants. Histopathology 1998 32 : 139 46.
10. Robinson JK. Margin control for lentigo maligna. J Am Acad Dermatol 1994 31 : 79 85.
11. Stonecipher MR, Leshin B, Patrick J, et al. Management of lentigo maligna and lentigo maligna melanoma with paraffin-embedded tangential sections: utility of immunoperoxidase staining and supplemental vertical sections. J Am Acad Dermatol 1993 29 : 589 94.
12. Menaker GM, Chiang JK, Tabila B, et al. Rapid HMB-45 staining in Mohs micrographic surgery for melanoma in situ and invasive melanoma. J Am Acad Dermatol 2001 44 : 833 6.
13. Gross EA, Andersen WK, Rogers GS. Mohs micrographic excision of lentigo maligna using Mel-5 for margin control. Arch Dermatol 1999 Jan 135 : 15 7.
14. de Vries TJ, Fourkour A, Wobbes T, et al. Heterogeneous expression of immunotherapy candidate proteins gp100, MART-1, and tyrosinase in human melanoma cell lines and in human melanocytic lesions. Cancer Res 1997 57 : 3223 9.
15. Busam KJ, Chen YT, Old LJ, et al. Expression of Melan-A (MART-1) in benign melanocytic nevi and primary cutaneous melanoma. Am J Surg Pathol 1998 22 : 976 82.
16. Jungbluth AA, Busam KJ, Gerald WL, et al. A103: an anti-Melan-A monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissue. Am J Surg Pathol 1998 22 : 595 602.
17. Shabrawi-Caelen LE, Kerl H, Cerroni L. Melan-A: not a helpful marker in distinction between melanoma in situ on sun-damaged skin and pigmented actinic keratosis. Am J Dermatopathol 2004 26 : 364 6.
18. Moore BW. A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 1965 19 : 739 44.
19. Vanstapel MJ, Gatter KC, Wolf-Peeters C, et al. New site of human S100 immunoreactivity detected with monoclonal antibodies. Am J Clin Pathol 1986 85 : 160 8.
20. Takahashi K, Isobe T, Ohtsuki Y, et al. Immunohistochemical study on the distribution of alpha and beta subunits of S100 protein in human neoplasms and normal tissues. Virchows Arch. (B) 1984 45 : 385 96.
21. Ruiter DJ, Brockner E. Immunohistochemistry in the evaluation of melanocytic tumors. Semin Diagn Pathol 1993 10 : 76 91.
22. Wick MR, Swanson PE, Rocamora A. Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors. J Cutan Pathol 1988 15 : 201 7.
23. Ordonez NG, Xiaolong JI, Hickey RC. Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma. Am J Clin Pathol 1988 90 : 385 90.
24. NCCN Clinical Practice Guidelines in Oncology. Melanoma. V.2. 2008, (accessed September 17, 2008).
25. Zitelli JA, Brown C, Hanusa BH. Mohs micrographic surgery for the treatment of primary cutaneous melanoma. J Am Acad Dermatol 1997 37 : 236 45.
26. Huilgol SC, Selva D, Chen C, et al. Surgical margins for lentigo maligna and lentigo maligna melanoma: the technique of mapped serial excision. Arch Dermatol 2004 140 : 1087 92.
27. Mohs FE. The width and depth of the spread of malignant melanoma as observed by a chemosurgeon. Am J Dermatopathol 1984 6 : 123 6.
28. Grande DJ, Koranda FC, Whitaker DC. Surgery of extensive subclinical lentigo maligna. J Dermatol Surg Oncol 1982 8 : 493 6.
29. Zitelli JA, Mohs FE, Larson P, et al. Mohs micrographic surgery for melanoma. Dermatol Clin 1989 7 : 833 43.
30. Breuninger H, Schlagenhauff B, Stroebel W, et al. Patterns of local horizontal spread of melanomas. Am J Surg Pathol 1999 23 : 1493 8.
31. Cohen LM, McCall W, Zax RH. Mohs micrographic surgery for lentigo maligna and lentigo maligna melanoma: a follow-up study. Dermatol Surg 1998 24 : 673 7.
32. Cohen LM, McCall MW, Hodge SJ, et al. Successful treatment of lentigo maligna and lentigo maligna melanoma with Mohs micrographic surgery aided by rush permanent sections. Cancer 1994 73 : 2964 70.
33. Coleman WP, Davis RS, Reed RJ, et al. Treatment of lentigo maligna and lentigo maligna melanoma. J Dermatol Surg Oncol 1980 6 : 476 9.
34. Pitman GH, Kopf AW, Bart RS, et al. Treatment of lentigo maligna and lentigo maligna melanoma. J Dermatol Surg Oncol 1979 5 : 727 37.
35. O'Brien CJ, Coates AS, Petersen-Schaefer K, et al. Experience with 998 cutaneous melanomas of the head and neck over 30 years. Am J Surg 1991 162 : 310 4.
36. Zitelli JA, Brown CD, Hanusa BH. Surgical margins for excision of primary cutaneous melanoma. J Am Acad Dermatol 1997 37 : 422 9.
37. Kelly JW, Sagabiel RW, Calderon W, et al. The frequency of local recurrence and microsatellites as a guide to reexcision margins for cutaneous malignant melanoma. Ann Surg 1984 200 : 759 63.
38. Hill DC, Gramp AA. Surgical treatment of lentigo maligna and lentigo maligna melanoma. Australas J Dermatol 1999 40 : 25 30.
39. Cochran AJ. Studies of the melanocytes of the epidermis adjacent to tumors. J Invest Dermatol 1971 57 : 38 43.
40. Wong CK. A study of melanocytes in the skin surrounding malignant melanomata. Dermatologica 1970 141 : 215 25.
41. Johnson TM, Smith JW, Nelson BR, et al. Current therapy for cutaneous melanoma. J Am Acad Dermatol 1995 32 : 689 707.
42. de Berker D. Lentigo maligna and Mohs [letter]. Arch Dermatol 1991 127 421.
43. Zitelli JA. Mohs surgery for lentigo maligna [letter]. Arch Dermatol 1991 127 : 1729 30.
44. Johnson TM, Headington JT, Baker SR, et al. Usefulness of the staged excision for lentigo maligna and lentigo maligna melanoma: the "square" procedure. J Am Acad Dermatol 1997 37 : 758 64.
45. Mohs FE. Chemosurgery for melanoma. Arch Dermatol 1977 113 : 285 91.
46. Mohs FE. Microscopically controlled surgery for periorbital melanoma: fixed-tissue and fresh-tissue techniques. J Dermataol Surg Oncol 1985 11 : 284 91.
47. Mohs FE. Fixed-tissue micrographic surgery for melanoma of the ear. Arch Otolaryngol Head Neck Surg 1988 114 : 625 31.
48. Mohs FE, Snow SN, Larson PO. Mohs micrographic surgery fixed-tissue technique for melanoma of the nose. J Dermatol Surg Oncol 1990 16 : 1111 20.
49. Snow SN, Mohs FE, Oriba HA, et al. Cutaneous malignant melanoma treated by Mohs surgery: review of the treatment results of 179 cases from the Mohs melanoma registry. Dermatol Surg 1997 23 : 1055 60.
50. Dhawan SS, Wolf DJ, Rabinovitz HS, et al. Lentigo maligna: the use of rush permanent sections in therapy. Arch Dermatol 1990 126 : 928 30.
51. Zitelli JA, Mohs FE, Larson P, et al. Mohs micrographic surgery for melanoma. Dermatol Clin 1989 833 43.
52. Robinson JK. Use of digital epiluminescence microscopy to help define the edge of lentigo maligna. Arch Dermatol 2004 140 : 1095 100.
53. Chen CS, Elias M, Busam K, et al. Multimodal in vivo optical imaging, including confocal microscopy, facilitates presurgical margin mapping for clinically complex lentigo maligna melanoma. Br J Dermatol 2005 153 : 1031 6.
54. Katz KH, Helm KF, Billingsley EM, et al. Dense inflammation does not mask residual primary basal cell carcinoma during Mohs micrographic surgery. J Am Acad Dermatol 2001 45 : 231 8.
55. Zachary CB, Rest EB, Furlong SM, et al. Rapid cytokeratin stains enhance the sensitivity of Mohs micrographic surgery for squamous cell carcinoma. J Dermatol Surg Oncol 1994 20 : 509 10.
56. Krunic AL, Garrod DR, Madani S, et al. Immunohistochemical staining for desmogleins 1 and 2 in keratinocytic neoplasms with squamous phenotype: actinic keratosis, keratoacanthoma and squamous cell carcinoma of the skin. Br J Cancer 1998 77 : 1275 9.
57. Krunic AL, Garrod DR, Viehman GE, et al. The use of antidesmoglein stains in Mohs micrographic surgery. A potential aid for the differentiation of basal cell carcinoma from horizontal sections of the hair follicle and folliculocentric basaloid proliferation. Dermatol Surg 1997 23 : 463 8.
58. Tope WD, Nowfar-Rad M, Kist DA. Ber-EP4-positive phenotype differentiates actinic keratosis from superficial basal cell carcinoma. Dermatol Surg 2000 26 : 415 8.
59. Swanson PE, Fitzpatrick MM, Ritter JH, et al. Immunohistologic differential diagnosis of basal cell carcinoma, squamous cell carcinoma and trichoepithelioma in small cutaneous biopsy specimens. J Cutan Pathol 1998 25 : 153 9.
60. Abdelsayed RA, Guijarro-Rojas M, Ibrahim NA, et al. Immunohistochemical evaluation of basal cell carcinoma and trichepithelioma using Bcl-2, Ki67, PCNA, and P53. J Cutan Pathol 2000 27 : 169 75.
61. Kirchmann TT, Prieto VG, Smoller BR. CD34 staining pattern distinguishes basal cell carcinoma from trichoepithelioma. Arch Dermatol 1994 120 : 589 92.
62. Kirchman TT, Prieto VG, Smoller BR. Use of CD34 in assessing the relationship between stroma and tumor in desmoplastic keratinocytic neoplasms. J Cutan Pathol 1995 22 : 422 6.
63. Chiller K, Passaro D, Scheuller M, et al. Microcystic adnexal carcinoma: forty-eight cases, their treatment, and their outcome. Arch Dermatol 2000 136 : 1355 9.
64. Khachemoune A, Olbricht SM, Johnson DS. Microcystic adnexal carcinoma: report of four cases treated with Mohs' micrographic surgical technique. Int J Dermatol 2005 44 : 507 12.
65. Leibovitch I, Huilgol SC, Selva D, et al. Microcystic adnexal carcinoma: treatment with Mohs micrographic surgery. J Am Acad Dermatol 2005 52 : 295 300.
66. Burns MK, Chen SP, Goldberg LH. Microcystic adnexal carcinoma. Ten cases treated by Mohs micrographic surgery. J Dermatol Surg Oncol 1994 20 : 429 37.
67. Nagatsuka H, Rivera RS, Gunduz M, et al. Microcystic adnexal carcinoma with mandibulary bone marrow involvement: a case report with Immunohistochemistry. Am J Dermatopathol 2006 28 : 518 22.
68. Requena L, Marquina A, Alegre V, et al. Sclerosing-sweat-duct (microcystic adnexal) carcinoma - a tumor from a single eccrine origin. Clin Exp Dermatol 1990 15 : 222 4.
69. Miyamoto T, Kambe N, Nishiura S, et al. Microcystic adnexal carcinoma. Electron microscopic and immunohistochemical study. Dermatologica 1990 180 : 40 3.
70. Uchida N, Urano Y, Oura H, et al. Microcystic adnexal carcinoma. Case report with an immunohistochemical study. Dermatology 1993 187 : 119 23.
71. Schwarze HP, Loche F, Lamant L, et al. Microcystic adnexal carcinoma induced by multiple radiation therapy. Int J Dermatol 2000 39 : 369 72.
72. Kato N, Yasuoka A, Ueno H. Microcystic adnexal carcinoma: a case report with immunohistochemical and electron microscopical examinations. J Dermatol 1992 19 : 51 7.
73. Spencer JM, Nossa R, Tse DT, et al. Sebaceous carcinoma of the eyelid treated with Mohs micrographic surgery. J Am Acad Dermatol 2001 44 : 1004 9.
74. Kass LG, Hornblass A. Sebaceous carcinoma of the ocular adnexa. Surv Ophthalmol 1989 33 : 477 90.
75. Snow SN, Larson PO, Lucarelli MJ, et al. Sebaceous carcinoma of the eyelids treated by Mohs micrographic surgery: report of nine cases with review of the literature. Dermatol Surg 2002 28 : 623 31.
76. Harvey JT, Anderson RL. The management of meibomian gland carcinoma. Ophthalmic Surg 1982 13 : 56 60.
77. Yount AB, Bylund D, Pratt SG, et al. Mohs micrographic excision of sebaceous carcinoma of the eyelids. J Dermatol Surg Oncol 1994 20 : 523 9.
78. Dzubow LM. Sebaceous carcinoma of the eyelid: treatment with Mohs surgery. J Dermatol Surg Oncol 1985 11 : 40 4.
79. Folberg R, Whitaker DC, Tse DT, et al. Recurrent and residual sebaceous carcinoma after Mohs excision of the primary lesion. Am J Ophthalmol 1987 103 : 817 23.
80. Ratz JL, Luu-Duong S, Kulwin DR. Sebaceous carcinoma of the eyelid treated with Mohs surgery. J Am Acad Dermatol 1986 14 : 668 73.
81. Dixon RS, Mikhail GR, Stater HC. Sebaceous carcinoma of the eyelid. J Am Acad Dermatol 1980 3 : 241 3.
82. Khan JA, Doane JF, Grove AS Jr. Sebaceous and meibomian carcinomas of the eyelid: recognition, diagnosis, and management. Ophthal Plast Reconstr Surg 1991 7 : 61 6.
83. Jakobiec FA, To KW. Sebaceous tumors of ocular adnexa. In : Albert DM, Jakobiec FA, Azar DT, et al., editors. Principles and practice of ophthalmology. Philadelphia : WB Saunders 2000. p. 3382 402.
84. Cabral ES, Auerbach A, Killian JK, et al. Distinction of benign sebaceous proliferations from sebaceous carcinomas by immunohistochemistry. Am J Dermatopathol 2006 28 : 465 71.
85. Sinard JH. Immunohistochemical distinction of ocular sebaceous carcinoma from basal cell and squamous cell carcinoma. Arch Ophthalmol 1999 117 : 776 83.
86. Mentzel T, Kutzner H, Rutten A, et al. Benign fibrous histiocytoma (dermatofibroma) of the face: clinicopathologic and immunohistochemical study of 34 cases associated with an aggressive clinical course. Am J Dermatopathol 2001 23 : 419 26.
87. Calonje E, Mentzel T, Fletcher CD. Cellular benign fibrous histiocytoma. Clinicopathologic analysis of 74 cases of a distinctive variant of cutaneous fibrous histiocytoma with frequent recurrence. Am J Surg Pathol 1994 18 : 668 76.
88. Gu M, Sohn K, Kim D, et al. Metastasizing dermatofibroma in lung. Ann Diagn Pathol 2007 11 : 64 7.
89. Colome-Grimmer MI, Evans HL. Metastasizing cellular dermatofibroma. A report of two cases. Am J Surg Pathol 1996 20 : 1361 7.
90. Kaddu S, McMenamin ME, Fletcher CD. Atypical fibrous histiocytoma of the skin: clinicopathologic analysis of 59 cases with evidence of infrequent metastasis. Am J Surg Pathol 2002 26 : 35 46.
91. Hsi ED, Nickoloff BJ. Dermatofibroma and dermatofibrosarcoma protuberans: an immunohistochemical study reveals distinctive antigenic profiles. J Dermatol Sci 1996 11 : 1 9.
92. Goldblum JR, Tuthill RJ. CD34 and factor-XIIIa immunoreactivity in dermatofibrosarcoma protuberans and dermatofibroma. Am J Dermatopathol 1997 19 : 147 53.
93. Abenoza P, Lillemoe T. CD34 and factor XIIIa in the differential diagnosis of dermatofibroma and dermatofibrosarcoma protuberans. Am J Dermatopathol 1993 15 : 429 34.
94. Garcia C, Clark RE, Buchanan M. Dermatofibrosarcoma protuberans. Int J Dermatol 1996 35 : 867 71.
95. Garcia C, Vichman G, Hitchcock M, et al. Dermatofibrosarcoma protuberans treated with Mohs surgery. A case with CD34 immunostaining variability. Dermatol Surg 1996 22 : 177 9.
96. Kahn HJ, Fekete E, From L. Tenascin differentiates dermatofibroma from dermatofibrosarcoma protuberans: comparison with CD34 and factor XIIIa. Hum Pathol 2001 32 : 50 6.
97. Li N, McNiff J, Hui P, et al. Differential expression of HMGA1 and HMGA2 in dermatofibroma and dermatofibrosarcoma protuberans: potential diagnostic applications and comparison with histologic findings, CD34, and factor XIIIa immunoreactivity. Am J Dermatopathol 2004 26 : 267 72.
98. Sachdev R, Sundram U. Expression of CD163 in dermatofibroma, cellular fibrous histiocytoma, and dermatofibrosarcoma protuberans: comparison with CD68, CD34, and Factor XIIIa. J Cutan Pathol 2006 33 : 353 60.
99. O'Connor WJ, Lim KK, Zalla MJ, et al. Comparison of Mohs micrographic surgery and wide excision for extramammary Paget's disease. Dermatol Surg 2003 29 : 723 7.
100. Davis J, Randle H, Zalla M, et al. A comparison of Mohs micrographic surgery and wide excision for the treatment of atypical fibroxanthoma. Dermatol Surg 1997 23 : 105 10.
101. Limmer B, Clark D. Cutaneous micrographic surgery for atypical fibroxanthoma. Dermatol Surg 1997 23 : 553 8.
102. Nouri K, Rivas M. A primer of mohs micrographic surgery: uncommon indications. Skin Med 2004 3 : 259 65.
103. Mirza B, Weedon D. Atypical fibroxanthoma: a clinicopathological study of 89 cases. Australas J Dermatol 2005 46 : 235 8.
104. Dudelzak J, Sheehan DJ, Mullins SC, et al. Malignant perifollicular atypical fibroxanthoma treated with Mohs surgery. Dermatol Surg 2007 33 : 364 8.
105. Thomas CJ, Wood GC, Marks VJ. Mohs micrographic surgery in the treatment of rare aggressive cutaneous tumors: the Geisinger experience. Dermatol Surg 2007 33 : 333 9.
106. Pathai S, Barlow R, Williams G, et al. Mohs' micrographic surgery for Merkel cell carcinoma of the eyelid. Orbit 2005 24 : 273 5.
107. Suarez C, Rodrigo JP, Ferlito A, et al. Merkel cell carcinoma of the head and neck. Oral Oncol 2004 40 : 773 9.
108. Boyer JD, Zitelli JA, Brodland DG, et al. Local control of primary Merkel cell carcinoma: a review of 45 cases treated with Mohs micrographic surgery with and without adjuvant radiation. J Am Acad Dermatol 2002 47 : 885 92.
109. Akhtar S, Oza KK, Wright J. Merkel cell carcinoma: report of 10 cases and review of the literature. J Am Acad Dermatol 2000 43 (5 Pt 1) : 755 67.
110. Koljonen V. Merkel cell carcinoma. World J Surg Oncol 2006 4 : 7.
111. Chan JK, Suster S, Wenig BM, et al. Cytokeratin 20 immunoreactivity distinguishes Merkel cell (primary cutaneous neuroendocrine) carcinomas and salivary gland small cell carcinomas from small cell carcinomas of various sites. Am J Surg Pathol 1997 21 : 226 34.
112. Cheuk W, Kwan MY, Suster S, et al. Immunostaining for thyroid transcription factor 1 and cytokeratin 20 aids the distinction of small cell carcinoma from Merkel cell carcinoma, but not pulmonary from extrapulmonary small cell carcinomas. Arch Pathol Lab Med 2001 125 : 228 31.
113. Wallace ML, Smoller BR. Immunohistochemistry in diagnostic dermatology. J Am Acad Dermatol 1996 34 : 163 83.
114. Bhardwaj SS, Tope WD, Lee PK. Mohs Micrographic surgery for lentigo maligna and lentigo maligna melanoma using Mel-5 immunostaining: University of Minnesota experience. Dermatol Surg 2006 32 : 690 6.
115. Bricca GM, Brodland DG, Zitelli JA. Immunostaining melanoma frozen sections: the 1-hour protocol. Dermatol Surg 2004 30 : 403 8.
116. Marra DE, Schanbacher CF, Torres A. Mohs micrographic surgery of primary cutaneous mucinous carcinoma using immunohistochemistry for margin control. Dermatol Surg 2004 30 : 799 802.
117. Tolland JP, Brenn T, Guldbakke KK, et al. Mohs micrographic surgery, sentinel lymph node mapping, and estrogen receptor analysis for the treatment of malignant nodular hidradenoma. Dermatol Surg 2006 32 : 1294 301.
118. Watanabe S, Ichikawa E, Takanashi S, et al. Immunohistochemical localization of cytokeratins in normal eccrine glands, with monoclonal antibodies in routinely processed, formalin-fixed, paraffin embedded sections. J Am Acad Dermatol 1993 28 : 203 12.
119. Benbenisty KM, Andrea A, Metcalf J, et al. Atypical cellular neurothekeoma treated with Mohs micrographic surgery. Dermatol Surg 2006 32 : 582 7.
120. Hu JC, Ko CJ, Soriano TT, et al. Syringomatous carcinoma in a young patient treated with Mohs micrographic surgery. Cutis 2006 77 : 19 24.
121. Allee JE, Cotsarelis G, Solky B, et al. Multiply recurrent trichilemmal carcinoma with perineural invasion and cytokeratin 17 positivity. Dermatol Surg 2003 29 : 886 9.
122. Hardaway CA, Graham BS, Barnette DJ, Feldman BD. Embryonal rhabdomyosarcoma presenting in an adult: a case report and discussion of immunohistochemical staining. Am J Dermatopath 2003 25 : 45 52.
123. Smith SB, Farley MF, Albertini JG, et al. Mohs micrographic surgery for granular cell tumor using S-100 immunostain. Dermatol Surg 2002 28 : 1076 8.
124. Gardner ES, Goldberg LH. Granular cell tumor treated with Mohs micrographic surgery: report of a case and review of the literature. Dermatol Surg 2001 27 : 772 4.
125. Dzubow LM, Kramer EM. Treatment of a large, ulcerating, granular-cell tumor by microscopically controlled excision. J Dermatol Surg Oncol 1985 11 : 392 5.
126. Albertini JG, Welsch MJ, Conger LA, et al. Infantile digital fibroma treated with Mohs micrographic surgery. Dermatol Surg 2002 28 : 959 61.
127. Kimyai-Asadi A, Ayala GB, Goldberg LH, et al. The 20-minute rapid MART-1 immunostain for malignant melanoma frozen sections. Dermatol Surg 2008 34 : 498 500.