A new real time PCR-based assay for diagnosing Renibacterium salmoninarum in rainbow trout (Oncorhynchus mykiss) and comparison with other techniques

Journal of Microbiological Methods

Volumn 76, Issue 1, 2009, Pages 75-80

  Halaihel, Nabil ^a   Vendrell, Daniel ^a   Ruiz Zarzuela, Imanol ^a   de Blas, Ignacio ^a   Alonso, José Luis ^a   Gironés, Olivia ^a   Pérez, Tania ^a   Muzquiz, José Luis ^a  

^a UNIVERSITY OF ZARAGOZA   (Spain)

Author keywords

ELISA; Immune Chromatography strip; Lux primers; Nested PCR; P57; Renibacterium salmoninarum; Reverse transcriptase Quantitative PCR

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; INTERMETHOD COMPARISON; KIDNEY DISEASE; NONHUMAN; PRIORITY JOURNAL; RAINBOW TROUT; REAL TIME POLYMERASE CHAIN REACTION; RENIBACTERIUM SALMONINARUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ACTINOMYCETALES INFECTIONS; ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FISH DISEASES; MICROCOCCACEAE; ONCORHYNCHUS MYKISS; POLYMERASE CHAIN REACTION; RANDOM ALLOCATION; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BACTERIA (MICROORGANISMS); ONCORHYNCHUS MYKISS; POSIBACTERIA; RENIBACTERIUM SALMONINARUM; SALMONIDAE;

EID: 57149126346     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.09.014     Document Type: Article

References (22)

1. Austin B., Embly T.M., and Goodfellow M. Selective isolation of Renibacterium salmoninarum. FEMS Microbiol. Lett. 17 (1983) 111-114
2. Bandin I., Santos Y., Barja J.I., and Toranzo A.E. Growth of the fish pathogen Renibacterium salmoninarum on different media. Microbiologia 12 3 (1996) 439-442
3. Bruno D.W. Prevalence and diagnosis of bacterial kidney disease (BKD) in Scotland between 1990 and 2002. Dis. Aquat. Organ. 59 2 (2004) 125-130
4. Coady A.M., Murray A.L., Elliott D.G., and Rhodes L.D. Both msa genes in Renibacterium salmoninarum are needed for full virulence in bacterial kidney disease. Appl. Environ. Microbiol. 72 4 (2006) 2672-2678
5. Cook M., and Lynch W.H. A sensitive nested reverse transcriptase PCR assay to detect viable cells of the fish pathogen Renibacterium salmoninarum in Atlantic salmon (Salmo salar L.). Appl. Environ. Microbiol. 65 7 (1999) 3042-3047
6. Chase D.M., and Pascho R.J. Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney. Dis. Aquat. Organ. 34 3 (1998) 223-229
7. Chase D.M., Elliott D.G., and Pascho R.J. Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis. J. Vet. Diagn. Invest. 18 4 (2006) 375-380
8. Grayson T.H., Cooper L.F., Wrathmell A.B., Roper J., Evenden A.J., and Gilpin M.L. Host responses to Renibacterium salmoninarum and specific components of the pathogen reveal the mechanisms of immune suppression and activation. Immunology 106 2 (2002) 273-283
9. Hamel O.S. Immunosuppression in progeny of chinook salmon infected with Renibacterium salmoninarum: re-analysis of a brood stock segregation experiment. Dis. Aquat. Organ 65 1 (2005) 29-41
10. Inglis V., Roberts R.J., and Bromage N.R. Bacterial diseases of fish (1993), Blackwell, Oxford (United Kingdom) 177-195
11. Konigsson M.H., Ballagi A., Jansson E., and Johansson K.E. Detection of Renibacterium salmoninarum in tissue samples by sequence capture and fluorescent PCR based on the 16S rRNA gene. Vet. Microbiol. 105 3-4 (2005) 235-243
12. Lowe B., Avila H.A., Bloom F.R., Gleeson M., and Kusser W. Quantitation of gene expression in neural precursors by reverse-transcription polymerase chain reaction using self-quenched, fluorogenic primers. Anal. Biochem. 315 1 (2003) 95-105
13. Magnusson H.B., Fridjonsson O.H., Andresson O.S., Benediktsdottir E., Gudmundsdottir S., and Andresdottir V. Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences. Appl. Environ. Microbiol. 60 12 (1994) 4580-4583
14. McIntosh D., Meaden P.G., and Austin B. A simplified PCR-based method for the detection of Renibacterium salmoninarum utilizing preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes. Appl. Environ. Microbiol. 62 11 (1996) 3929-3932
15. Meyers T.R., Thrower F., Short S., Lipson K., Joyce J., Farrington C., et al. Different prevalences of Renibacterium salmoninarum detected by ELISA in Alaskan chinook salmon Oncorhynchus tshawytscha spawned from freshwater and seawater. Dis. Aquat. Organ. 35 2 (1999) 101-105
16. Miriam A., Griffiths S.G., Lovely J.E., and Lynch W.H. PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen Renibacterium salmoninarum. J. Clin. Microbiol. 35 6 (1997) 1322-1326
17. Nazarenko I., Lowe B., Darfler M., Ikonomi P., Schuster D., and Rashtchian A. Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore. Nucleic Acids Res. 30 9 (2002) e37
18. Piganelli J.D., Wiens G.D., Zhang J.A., Christensen J.M., and Kaattari S.L. Evaluation of a whole cell, p57-vaccine against Renibacterium salmoninarum. Dis. Aquat. Organ 36 1 (1999) 37-44
19. Powell M., Overturf K., Hogge C., and Johnson K. Detection of Renibacterium salmoninarum in chinook salmon, Oncorhynchus tshawytscha (Walbaum), using quantitative PCR. J. Fish Dis. 28 10 (2005) 615-622
20. Rhodes L.D., Nilsson W.B., and Strom M.S. Sensitive detection of Renibacterium salmoninarum in whole fry, blood, and other tissues of pacific salmon by reverse transcription-polymerase chain reaction. Mol. Mar. Biol. Biotechnol. 7 4 (1998) 270-279
21. Suzuki K., and Sakai D.K. Real-time PCR for quantification of viable Renibacterium salmoninarum in chum salmon Oncorhynchus keta. Dis. Aquat. Organ 74 3 (2007) 209-223
22. Wiens G.D., Chien M.S., Winton J.R., and Kaattari S.L. Antigenic and functional characterization of p57 produced by Renibacterium salmoninarum. Dis. Aquat. Organ 37 1 (1999) 43-52