HPTLC/DESI-MS imaging of tryptic protein digests separated in two dimensions

Journal of Mass Spectrometry

Volumn 43, Issue 12, 2008, Pages 1627-1635

  Pasilis, Sofie P ^a   Kertesz, Vilmos ^a   Van Berkel, Gary J ^a   Schulz, Michael ^b   Schorcht, Susanne ^b  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b MERCK KGAA   (Germany)

Author keywords

DESI; HPTLC; Imaging; Mass spectrometry; Peptides

Indexed keywords

CELLULOSE DERIVATIVES; DESORPTION; ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY; PEPTIDES; PROTEINS; SEARCH ENGINES; SEMICONDUCTOR QUANTUM DOTS; SPECTROMETERS; SPECTROMETRY; SPECTRUM ANALYSIS; TWO DIMENSIONAL;

CELLULOSE SHEETS; CYTOCHROME C; DESI; DESORPTION ELECTROSPRAY IONIZATION MASS SPECTROMETRIES; HPTLC; IMAGING; MULTIPLE CHARGING; PEPTIDE IDENTIFICATIONS; PROTEIN DATABASE SEARCHES; PROTEIN DIGESTS; SEQUENCE COVERAGES; TANDEM MASS SPECTRUMS; TLC PLATES; TRYPTIC DIGESTS; TWO DIMENSIONS;

AMINES;

CYTOCHROME C; MYOGLOBIN;

ARTICLE; DATA BASE; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; MASS SPECTROMETER; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY, LIQUID; CYTOCHROMES C; HORSES; MOLECULAR SEQUENCE DATA; MYOGLOBIN; PEPTIDES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN;

AMINES; CELLULOSE DERIVATIVES; CHARGING; COMPUTER PROGRAMS; DESORPTION; IMAGE ANALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; PEPTIDES; PROTEINS; SPECTROMETERS; SPECTROSCOPY;

EID: 57149116942     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1431     Document Type: Article

References (30)

1. Takáts Z, Wiseman JM, Gologan B, Cooks RG. Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 2004; 306: 471.
2. Takáts Z, Wiseman JM, Cooks RG. Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology. Journal of Mass Spectrometry 2005; 40: 1261.
3. Cooks RG, Ouyang Z, Takáts Z, Wiseman JM. Ambient mass spectrometry. Science 2006; 311: 1566.
4. Van Berkel GJ, Ford MJ, Deibel MA. Thin-layer chromatography and mass spectrometry coupled using desorption electrospray ionization. Analytical Chemistry 2005; 77: 1207.
5. Van Berkel GJ, Kertesz V. Automated sampling and imaging of analytes separated on thin-layer chromatography plates using desorption electrospray ionizationmass spectrometry. Analytical Chemistry 2006; 78: 4938.
6. Kauppila TJ, Talaty N, Salo PK, Kotiaho T, Kostiainen R, Cooks RG. New surfaces for desorption electrospray ionization mass spectrometry: porous silicon and ultra-thin layer chromatography plates. Rapid Communications in Mass Spectrometry 2006; 20: 2143.
7. Van Berkel GJ, Tomkins BA, Kertesz V. Thin-layer chromatography/ desorption electrospray ionization mass spectrometry: investigation of goldenseal alkaloids. Analytical Chemistry 2007; 79: 2778.
8. Myung S, Wiseman JM, Valentine SJ, Takáts Z, Cooks RG, Clemmer DE. Coupling desorption electrospray ionization with ionmobility/mass spectrometry for analysis of protein structure: evidence for desorption of folded and denatured states. Journal of Physical Chemistry 2006; 110: 5045.
9. Bereman MS, Nyadong L, Fernandez FM, Muddiman DC. Direct high-resolution peptide and protein analysis by desorption electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry 2006; 20: 3409.
10. Shin Y-S, Drolet B, Mayer R, Dolence K, Basile F. Desorption electrospray ionizationmass spectrometry of proteins. Analytical Chemistry 2007; 79: 3514.
11. Kaur-Atwal G, Weston DJ, Green PS, Crosland S, Bonner PLR, Creaser CS. Analysis of tryptic peptides using desorption electrospray ionization combined with ion mobility spectrometry/mass spectrometry. Rapid Communications in Mass Spectrometry 2007; 21: 1131.
12. Cotte-Rodriguez I, Mulligan CC, Cooks RG. Non-proximate detection of small and large molecules by desorption electrospray ionization and desorption atmospheric pressure chemical ionzation mass spectrometry: instrumentation and applications in forensics, chemistry, and biology. Analytical Chemistry 2007; 79: 7069.
13. Pasilis SP, Kertesz V, Van Berkel GJ, Schulz M, Schorcht S. Using HPTLC/DESI-MS for peptide identification in 1D separations of tryptic protein digests. Analytical and Bioanalytical Chemistry 2008; 391: 317.
14. Poole CF, Poole SK. Multidimensionality in planar chromatography. Journal of Chromatography A 1995; 703: 573.
15. Nyiredy S. Multidimensional planar chromatography. In Multidimensional Chromatography, Chapter 8, Mondello L, Lewis AC, Bartle KD (eds). Wiley: New York, 2002; 171.
16. Patton WF, Panchagnula V, Rockney E, Krull IS. Taking a walk on the wild side with planar electrochromatography and thin-layer electrophoresis: of peptides, proteins, and proteomics. Journal of Liquid Chromatography and Related Technologies 2006; 29: 1177.
17. Wiseman JM, Ifa DR, Song QY, Cooks RG. Tissue imaging at atmospheric pressure using desorption electrospray ionization (DESI) mass spectrometry. Angewandte Chemie International Edition 2006; 45: 7188.
18. Ifa DR, Wiseman JM, Song QY, Cooks RG. Development of capabilities for imaging mass spectrometry under ambient conditions with desorption electrospray ionization (DESI). International Journal of Mass Spectrometry 2007; 259: 8.
19. Ifa DR, Gumaelius LM, Eberlin LS, Manicke NE, Cooks RG. Forensic analysis of inks by imaging desorption electrospray ionization (DESI) mass spectrometry. Analyst 2007; 132: 461.
20. Wysocki VH, Resing KA, Zhang Q, Cheng G. Mass spectrometry of peptides and proteins. Methods 2005; 35: 211.
21. McDonald WH, Tabb DL, Sadygov RG, MacCoss MJ, Venable J, Graumann J, Johnson JR, Cociorva D, Yates JR III. MS1, MS2, and SQT - three unified, compact, and easily parsed file formats for the storage of shotgun proteomic spectra and identifications. Rapid Communications in Mass Spectrometry 2004; 18: 2162.
22. Tabb DL, Narasimhan C, Strader BL, Hettich RL. DBDigger: reorganized proteomic database identification that improves flexibility and speed. Analytical Chemistry 2005; 77: 2464.
23. Narasimhan C, Tabb DL, VerBerkmoes NC, Thompson MR, Hettich RL, Uberbacher EC. MASPIC: intensity-based tandem mass spectrometry scoring scheme that improves peptide identification at high confidence. Analytical Chemistry 2005; 77: 7581.
24. Tabb DL, McDonald WH, Yates JR. DTASelect and contrast: tools for assembling and comparing protein identifications from shotgun proteomics. Journal of Proteome Research 2002; 1: 21.
25. Dumbleton JP, Pasilis SP, Kertesz V, Van Berkel GJ, Unpublished results.
26. Cech NB, Enke CG. Relating electrospray ionization response to nonpolar character of small peptides. Analytical Chemistry 2000; 72: 2717.
27. Huang Y, Triscari JM, Tseng GC, Pasa-Tolic L, Lipton MS, Smith RD, Wysocki VH. Statistical characterization of the charge state and residue dependence of low-energy CID peptide dissociation patterns. Analytical Chemistry 2005; 77: 5800.
28. Rollgen FW, Borchers F, Giessmann U, Levsen K. Collisional activation of ions formed by [Li]+ ion attachment. Organic Mass Spectrometry 1977; 12: 541.
29. Iavarone AT, Udekwu OA, Williams ER. Buffer loading for counteracting metal salt-induced signal supression in electrospray ionization. Analytical Chemistry 2004; 76: 3944.
30. Jackson AU, Talaty N, Cooks RG, Van Berkel GJ. Salt tolerance of desorption electrospray ionization. Journal of the American Society for Mass Spectrometry 2007; 18: 2218.