Large generation of megakaryocytes from serum-free expanded human CD34+ cells

Biochemical and Biophysical Research Communications

Volumn 378, Issue 1, 2009, Pages 112-117

  Chen, Te Wei ^a,b   Yao, Chao Ling ^a   Chu, I Ming ^b   Chuang, Tzu Lin ^a   Hsieh, Tzu Bou ^a   Hwang, Shiaw Min ^a  

^a FOOD INDUSTRY RESEARCH AND DEVELOPMENT INSTITUTE   (Taiwan)

^b NATIONAL TSING HUA UNIVERSITY   (Taiwan)

Author keywords

Hematopoietic stem cells; Induction; Megakaryocytes; Serum free

Indexed keywords

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 3; INTERLEUKIN 6; INTERLEUKIN 9;

ARTICLE; CD34 CELL; CELL; CONTROLLED STUDY; CULTURE MEDIUM; CYTOKINE PRODUCTION; EX VIVO STUDY; FACTORIAL DESIGN; GENE EXPRESSION; HUMAN; HUMAN CELL; MEGAKARYOCYTE; MEGAKARYOPOIESIS; POLYPLOIDY; PRIORITY JOURNAL; PROTEIN INDUCTION; SCREENING; THROMBOCYTE ACTIVATION;

ANTIGENS, CD34; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA, SERUM-FREE; CYTOKINES; HEMATOPOIETIC STEM CELLS; HUMANS; MEGAKARYOCYTES; PLATELET ACTIVATION; PLOIDIES; RECOMBINANT PROTEINS;

EID: 56949100092     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.11.019     Document Type: Article

References (25)

1. Levine R.F. Isolation and characterization of normal human megakaryocytes. Br. J. Haematol. 45 (1980) 487-497
2. Levine R.F., Shoff P., Han Z.C., and Eldor A. Circulating megakaryocytes and platelet production in the lungs. Prog. Clin. Biol. Res. 356 (1990) 41-52
3. Miyazaki R., Ogata H., Iguchi T., Sogo S., Kushida T., Ito T., Inaba M., Ikehara S., and Kobayashi Y. Comparative analyses of megakaryocytes derived from cord blood and bone marrow. Br. J. Haematol. 108 (2000) 602-609
4. + cells. Ann. Hematol. 79 (2000) 13-19
5. George J.N. Platelets. Lancet 355 (2000) 1531-1539
6. Cairo M.S., and Wagner J.E. Placental and/or umbilical cord blood: an alternative source of hematopoietic stem cells for transplantation. Blood 90 (1997) 4665-4678
7. Kanamaru S., Kawano Y., Watanabe T., Nakagawa R., Suzuya H., Onishi T., Yamazaki J., Nakayama T., Kuroda Y., and Takaue Y. Low numbers of megakaryocyte progenitors in grafts of cord blood cells may result in delayed platelet recovery after cord blood cell transplant. Stem Cells 18 (2000) 190-195
8. + cells. Stem Cells 22 (2004) 135-143
9. Bertolini F., Battaglia M., Pedrazzoli P., Da Prada G.A., Lanza A., Soligo D., Caneva L., Sarina B., Murphy S., Thomas T., and della Cuna G.R. Megakaryocytic progenitors can be generated ex vivo and safely administered to autologous peripheral blood progenitor cell transplant recipients. Blood 89 (1997) 2679-2688
10. Taguchi K., Saitoh M., Arai Y., Momose K., Ogawa Y., Yasuda S., and Miyata K. Disparate effects of interleukin 11 and thrombopoietin on megakaryocytopoiesis in vitro. Cytokine 15 (2001) 241-249
11. Basser R.L., Rasko J.E., Clarke K., Cebon J., Green M.D., Hussein S., Alt C., Menchaca D., Tomita D., Marty J., Fox R.M., and Begley C.G. Thrombopoietic effects of pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) in patients with advanced cancer. Lancet 348 (1996) 1279-1281
12. Briddell R.A., Bruno E., Cooper R.J., Brandt J.E., and Hoffman R. Effect of c-kit ligand on in vitro human megakaryocytopoiesis. Blood 78 (1991) 2854-2859
13. Nagler A., Eldor A., Naparstek E., Mumcuoglu M., Slavin S., and Deutsch V.R. Ex vivo expansion of megakaryocyte precursors by preincubation of marrow allografts with interleukin-3 and granulocyte-macrophage colony-stimulating factor in vitro. Exp. Hematol. 23 (1995) 1268-1274
14. Bruno E., and Hoffman R. Effect of interleukin 6 on in vitro human megakaryocytopoiesis: its interaction with other cytokines. Exp. Hematol. 17 (1989) 1038-1043
15. Piacibello W., Garetto L., Sanavio F., Severino A., Fubini L., Stacchini A., Dragonetti G., and Aglietta M. The effects of human FLT3 ligand on in vitro human megakaryocytopoiesis. Exp. Hematol. 24 (1996) 340-346
16. Fujiki H., Kimura T., Minamiguchi H., Harada S., Wang J., Nakao M., Yokota S., Urata Y., Ueda Y., Yamagishi H., and Sonoda Y. Role of human interleukin-9 as a megakaryocyte potentiator in culture. Exp. Hematol. 30 (2002) 1373-1380
17. Yao C.L., Liu C.H., Chu I.M., Hsieh T.B., and Hwang S.M. Factorial designs combined with the steepest ascent method to optimize serum-free media for ex vivo expansion of human hematopoietic progenitor cells. Enzyme Microb. Tech. 33 (2003) 343-352
18. + cells. Stem Cells Dev. 15 (2006) 70-78
19. + cells. Stem Cells Dev. 16 (2007) 1043-1051
20. + cells are correlated with different levels of platelet release. Blood 99 (2002) 888-897
21. Giannini E.G. Review article: thrombocytopenia in chronic liver disease and pharmacologic treatment options. Aliment Pharmacol. Ther. 23 (2006) 1055-1065
22. Bruno S., Gunetti M., Gammaitoni L., Dane A., Cavalloni G., Sanavio F., Fagioli F., Aglietta M., and Piacibello W. In vitro and in vivo megakaryocyte differentiation of fresh and ex-vivo expanded cord blood cells: rapid and transient megakaryocyte reconstitution. Haematologica 88 (2003) 379-387
23. Schipper L.F., Brand A., Reniers N., Melief C.J., Willemze R., and Fibbe W.E. Differential maturation of megakaryocyte progenitor cells from cord blood and mobilized peripheral blood. Exp. Hematol. 31 (2003) 324-330
24. + cells derived from fetal liver contained a high proportion of immature megakaryocytic progenitor cells. Eur. J. Haematol. 64 (2000) 304-314
25. Cortin V., Garnier A., Pineault N., Lemieux R., Boyer L., and Proulx C. Efficient in vitro megakaryocyte maturation using cytokine cocktails optimized by statistical experimental design. Exp. Hematol. 33 (2005) 1182-1191