Structure-function relationships in human salivary α-amylase: Role of aromatic residues in a secondary binding site

Biologia

Volumn 63, Issue 6, 2008, Pages 1028-1034

  Ragunath, Chandran ^a   Manuel, Suba G A ^a   Kasinathan, Chinnasamy ^a   Ramasubbu, Narayanan ^a  

^a UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

Author keywords

Bacterial binding; Crystal structure; Oligosaccharide hydrolysis; Salivary amylase; Site directed mutagenesis; Subsite engineering

Indexed keywords

BACTERIA (MICROORGANISMS); STREPTOCOCCUS; STREPTOCOCCUS GORDONII;

EID: 56949089568     PISSN: 00063088     EISSN: 13369563     Source Type: Journal    
DOI: 10.2478/s11756-008-0163-3     Document Type: Article

References (33)

1. H. Berman K. Henrick H. Nakamura J.L. Markley 2007 The worldwide Protein Data Bank (wwPDB): ensuring a single, uniform archive of PDB data Nucleic Acids Res. 35 D301 D303
2. P. Bernfeld 1955 Amylase, α and β Methods Enzymol. 1 49 150
3. G.D. Brayer Y. Luo S.G. Withers 1995 The structure of human pancreatic α-amylase at 1.8 Å resolution and comparisons with related enzymes Protein Sci. 4 1730 1742
4. A.E. Brown J.D. Rogers E.M. Haase P.M. Zelasko F.A. Scannapieco 1999 Prevalence of the amylase-binding protein A gene (abpA) in oral streptococci J. Clin. Microbiol. 37 4081 4085
5. CCP4. 1994 The CCP4 Suite: Programs for Protein Crystallography Acta Cryst. D50 760 763
6. C.W. Douglas 1983 The binding of human salivary α-amylase by oral strains of streptococcal bacteria Arch. Oral. Biol. 28 567 573
7. C.W. Douglas A.A. Pease R.A. Whiley 1990 Amylase-binding as a discriminator among oral streptococci FEMS Microbiol. Lett. 54 193 197
8. P. Emsley K. Cowtan 2004 Coot: model building tools for molecular graphics Acta Cryst. D60 2126 2132
9. F.C. Greenwood W.M. Hunter J.S. Glover 1963 The preparation of I-131-labelled human growth hormone of high specific radioactivity Biochem J. 89 114 123
10. J.P. Gwynn C.W. Douglas 1994 Comparison of amylase-binding proteins in oral streptococci FEMS Microbiol. Lett. 124 373 379
11. P. Handley A. Coykendall D. Beighton J.M. Hardie R.A. Whiley 1991 Streptococcus crista sp. nov., a viridans streptococcus with tufted fibrils, isolated from the human oral cavity and throat Int. J. Syst. Bacteriol. 41 543 547
12. L. Kandra G. Gyemant J. Remenyik C. Ragunath N. Ramasubbu 2003 Subsite mapping of human salivary α-amylase and the mutant Y151M FEBS Lett. 544 194 198
13. V.S. Lamzin K.S. Wilson 1993 Automated refinement of protein models Acta Cryst. D49 129 149
14. R.A. Laskowski M.W. Macarthur D.S. Moss J.M. Thornton 1993 PROCHECK: a program to check the stereochemical quality of protein structures J. Appl. Cryst. 26 283 291
15. C.Y. Loo D.A. Corliss N. Ganeshkumar 2000 Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes J. Bacteriol. 182 1374 1382
16. E.A. MacGregor S. Janecek B. Svensson 2001 Relationship of sequence and structure to specificity in the α-amylase family of enzymes Biochim. Biophys. Acta 1546 1 20
17. P.J. Mishra C. Ragunath N. Ramasubbu 2002 The mechanism of salivary amylase hydrolysis: role of residues at subsite S2' Biochem. Biophys. Res. Commun. 292 468 473
18. Z. Otwinowski W. Minor 1997 Processing of X-ray crystallographic data in oscillation mode Methods Enzymol. 276 307 326
19. C. Ragunath K. Sundar N. Ramasubbu 2002 Expression, characterization, and biochemical properties of recombinant human salivary amylase Protein Expr. Purif. 24 202 211
20. N. Ramasubbu K.K. Bhandary F.A. Scannapieco M.J. Levine 1991 Crystallization and preliminary X-ray diffraction studies of human salivary α-amylase Proteins 11 230 232
21. N. Ramasubbu V. Paloth Y. Luo G.D. Brayer M.J. Levine 1996 Structure of human salivary α-amylase at 1.6 Å resolution: implications for its role in the oral cavity Acta Cryst. D52 435 446
22. N. Ramasubbu C. Ragunath P.J. Mishra 2003 Probing the role of a mobile loop in substrate binding and enzyme activity of human salivary amylase J. Mol. Biol. 325 1061 1076
23. N. Ramasubbu C. Ragunath P.J. Mishra L.M. Thomas G. Gyemant L. Kandra 2004 Human salivary α-amylase Trp58 situated at subsite -2 is critical for enzyme activity Eur. J. Biochem. 271 2517 2529
24. J. Remenyik C. Ragunath N. Ramasubbu G. Gyemant A. Liptak L. Kandra 2003 Introducing transglycosylation activity into human salivary α-amylase (HSA) Org. Lett. 5 4895 4898
25. J.D. Rogers E.M. Haase A.E. Brown C.W. Douglas J.P. Gwynn F.A. Scannapieco 1998 Identification and analysis of a gene (abpA) encoding a major amylase-binding protein in Streptococcus gordonii Microbiology 144 1223 1233
26. J.D. Rogers R.J. Palmer Jr. P.E. Kolenbrander F.A. Scannapieco 2001 Role of Streptococcus gordonii amylase-binding protein A in adhesion to hydroxyapatite, starch metabolism, and biofilm formation Infect. Immun. 69 7046 7056
27. F.A. Scannapieco E.J. Bergey M.S. Reddy M.J. Levine 1989 Characterization of salivary α-amylase binding to Streptococcus sanguis Infect. Immun. 57 2853 2863
28. F.A. Scannapieco K. Bhandary N. Ramasubbu M.J. Levine 1990 Structural relationship between the enzymatic and streptococcal binding sites of human salivary α-amylase Biochem. Biophys. Res. Commun. 173 1109 1115
29. F.A. Scannapieco G.G. Haraszthy M.I. Cho M.J. Levine 1992 Characterization of an amylase-binding component of Streptococcus gordonii G9B Infect. Immun. 60 4726 4733
30. F.A. Scannapieco L. Solomon R.O. Wadenya 1994 Emergence in human dental plaque and host distribution of amylase-binding streptococci J Dent. Res. 73 1627 1635
31. F.A. Scannapieco G. Torres M.J. Levine 1993 Salivary α-amylase: role in dental plaque and caries formation Crit. Rev. Oral Biol. Med. 4 301 307
32. F.A. Scannapieco G.I. Torres M.J. Levine 1995 Salivary amylase promotes adhesion of oral streptococci to hydroxyapatite J. Dent. Res. 74 1360 1366
33. C.C. Tseng F.A. Scannapieco M.J. Levine 1992 Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions Oral Microbiol. Immunol. 7 53 56