Buffer optimization for high resolution of human lung cancer tissue proteins by two-dimensional gel electrophoresis

Biotechnology Letters

Volumn 31, Issue 1, 2009, Pages 31-37

  Lee, Kibeom ^a   Pi, Kyungbae ^b   Lee, Keeman ^c  

^a SongDo Techno Park ^*  (South Korea)

^b AdipoGen Inc   (South Korea)

^c Sunchon National University   (South Korea)

Author keywords

Protein resolution; Solubilization; Two dimensional gel electrophoresis

Indexed keywords

BIOLOGICAL ORGANS; COLLOIDS; ELECTROCHEMISTRY; GELATION; GELS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAMMALS; MASS SPECTROMETRY; NUCLEAR PROPULSION; PROTEINS; SOLUBILITY; THIOUREAS; TWO DIMENSIONAL; UREA;

COMPLEX COMPONENTS; GEL ANALYSIS; GEL ELECTROPHORESIS; HIGH RESOLUTIONS; HUMAN LUNGS; ISOELECTRIC FOCUSING; LUNG CANCERS; LUNG CARCINOMATA; METHODOLOGICAL TOOLS; OPTIMIZED CONDITIONS; PROTEIN COMPONENTS; PROTEIN SPOTS; PROTEOMES; PROTEOMIC ANALYSIS; SOLUBILIZATION; SQUAMOUS CELL CARCINOMATA; SUPERIOR RESOLUTIONS; TRITON X-100; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS;

BUFFER; DETERGENT; DITHIOTHREITOL; REDUCING AGENT; THIOUREA; TUMOR PROTEIN;

ARTICLE; CHEMISTRY; DRUG EFFECT; HUMAN; ISOELECTRIC FOCUSING; LUNG TUMOR; METHODOLOGY; SOLUBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BUFFERS; DETERGENTS; DITHIOTHREITOL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; ISOELECTRIC FOCUSING; LUNG NEOPLASMS; NEOPLASM PROTEINS; REDUCING AGENTS; SOLUBILITY; THIOUREA;

MAMMALIA;

EID: 56649117460     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9837-8     Document Type: Article

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