MethyQESD, a robust and fast method for quantitative methylation analyses in HNPCC diagnostics using formalin-fixed and paraffin-embedded tissue samples

Laboratory Investigation

Volumn 88, Issue 12, 2008, Pages 1367-1375

  Bettstetter, Marcus ^a   Dechant, Stefan ^a   Ruemmele, Petra ^a   Vogel, Corinna ^a   Kurz, Katrin ^a   Morak, Monika ^b   Keller, Gisela ^c   Holinski Feder, Elke ^b,d   Hofstaedter, Ferdinand ^a   Dietmaier, Wolfgang ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^d Medical Genetic Center   (Germany)

Author keywords

BRAF mutation; HNPCC; Microsatellite instability; MLH1; MSI; Quantitative methylation analysis

Indexed keywords

BISULFITE; PARAFFIN;

ADULT; AGED; ARTICLE; CANCER CELL CULTURE; COLORECTAL CANCER; DIAGNOSTIC ACCURACY; DIAGNOSTIC APPROACH ROUTE; DIAGNOSTIC TEST; DNA DETERMINATION; DNA METHYLATION; HUMAN; HUMAN CELL; HUMAN TISSUE; METHYLATION QUANTIFICATION OF ENDONUCLEASE RESISTANT DNA; MICROSATELLITE MARKER; MISMATCH REPAIR; MOLECULAR PROBE; PRIORITY JOURNAL; PROMOTER REGION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECEIVER OPERATING CHARACTERISTIC; TISSUE FIXATION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; BASE SEQUENCE; COLORECTAL NEOPLASMS, HEREDITARY NONPOLYPOSIS; DNA METHYLATION; DNA PRIMERS; FORMALDEHYDE; HUMANS; NUCLEAR PROTEINS; PARAFFIN EMBEDDING; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS; ROC CURVE; TISSUE FIXATION;

EID: 56649097479     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2008.100     Document Type: Article

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