Viability and membrane integrity of spermatozoa after dilution and flow cytometric sorting in the presence or absence of seminal plasma

Reproduction, Fertility and Development

Volumn 8, Issue 8, 1996, Pages 1165-1178

  Maxwell, W M C ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CATTLE; CELL MEMBRANE; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; MALE; NONHUMAN; SEMINAL PLASMA; SHEEP; SPERMATOZOON; SWINE;

ANIMALS; CATTLE; CELL MEMBRANE; CELL SURVIVAL; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; MALE; OSMOLAR CONCENTRATION; SEMEN; SHEEP; SPERM MOTILITY; SPERMATOZOA; SWINE;

EID: 0030442287     PISSN: 10313613     EISSN: None     Source Type: Journal    
DOI: 10.1071/RD9961165     Document Type: Article

References (6)

1. Ashworth, P. J. C., Harrison, R. A. P., Miller, N. G. A., Plummer, J. M., and Watson, P. F. (1994). Survival of ram spermatozoa at high dilution: protective effect of simple constituents of culture media as compared with seminal plasma. Reprod. Fertil. Dev. 6, 173-80.
2. Ashworth, P. J. C., Harrison, R. A. P., Miller, N. G. A., Plummer, J. M., and Watson, P. F. (1995). Flow cytometric detection of bicarbonate-induced changes in lectin binding in boar and ram sperm populations. Mol. Reprod. Dev. 40, 164-76.
3. Byrd, W., and Wolf, D. (1986). Acrosomal status in fresh and capacitated human ejaculated sperm. Biol. Reprod. 34, 859-69.
4. Calvete, J. J., Sanz, L., Enßlin, M., and Töpfer-Petersen, E. (1996). Sperm surface proteins. In 'Boar Semen Preservation. III'. Reprod. Dom. Anim. 31, 101-5.
5. Catt, S. L., O'Brien, J. K., Maxwell, W. M. C., and Evans, G. (1995). Viability of ram and boar spermatozoa during cell-sorting by flow cytometry. Proc. Aust. Soc. Reprod. Biol. 27, 49. [Abstr.]
6. Chang, M. (1957). A detrimental effect of seminal plasma on the fertilizing capacity of sperm. Nature (Lond.) 179, 258-9.