Silent mutations result in HlyA hypersecretion by reducing intracellular HlyA protein aggregates

Biotechnology and Bioengineering

Volumn 101, Issue 5, 2008, Pages 967-974

  Gupta, Prateek ^a,b   Lee, Kelvin H ^b,c  

^a Cornell University   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

^c University of Delaware   (United States)

Author keywords

hemolysin; Hemolysin pathway; Rare codon; Type I

Indexed keywords

AGGLOMERATION; BLOOD; ENZYME ACTIVITY; ESCHERICHIA COLI; PROTEINS; TRANSLATION (LANGUAGES);

COMPARATIVE ANALYSIS; CONTROL STRAINS; EXTRACELLULAR PROTEINS; HEMOLYSIN PATHWAY; HYPERSECRETION; INCLUSION BODIES; INTRACELLULAR LEVELS; MUTANT STRAINS; PARENT STRAINS; PROTEIN AGGREGATES; PROTEIN AGGREGATIONS; RARE CODON; RECOMBINANT PROTEINS; SECRETED PROTEINS; SILENT MUTATIONS; TRANSLATION RATES; TYPE-I;

STRAIN;

ALPHA HEMOLYSIN;

ARTICLE; CELL INCLUSION; CODON; CONTROLLED STUDY; ESCHERICHIA COLI; GENE; GENE MUTATION; HLYA GENE; NONHUMAN; PROTEIN AGGREGATION; PROTEIN PURIFICATION; PROTEIN SECRETION;

BIOLOGICAL TRANSPORT; CODON; CYTOPLASM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HEMOLYSIN PROTEINS; INCLUSION BODIES; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 56449096169     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21979     Document Type: Article

References (27)

1. Blight MA, Holland IB. 1994. Heterologous protein secretion and the versatile Escherichia coli haemolysin translocator. Trend Biotechnol 12:450-455.
2. Chapman E, Farr GW, Usaite R, Furtak K, Fenton WA, Chaudhuri TK, Hondorp ER, Matthews RG, Wolf SG, Yates JR, Pypaert M, Horwich AL. 2006. Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL. Proc Natl Acad Sci USA 103:15800-15805.
3. Felmlee T, Pellett S, Lee EY, Welch RA. 1985a. Escherichia coli hemolysin is released extracellularly without cleavage of a signal peptide. J Bacteriol 163:88-93.
4. Felmlee T, Pellett S, Welch RA. 1985b. Nucleotide sequence of an Escherichia coli chromosomal hemolysin. J Bacteriol 163:94-105.
5. Felmlee T, Welch RA. 1988. Alterations of amino acid repeats in the Escherichia coli hemolysin affect cytolytic activity and secretion. Proc Natl Acad Sci USA 85:5269-5273.
6. Georgiou G, Telford JN, Shuler ML, Wilson DB. 1986. Localization of inclusion bodies in Escherichia coli overproducing beta-lactamase or alkaline phosphatase. Appl Environ Microbiol 52:1157-1161.
7. Ikemura T. 1981. Correlation between the abundance of E. coli transfer RNAs and the occurrence of the respective codon in the protein genes. J Mol Biol 146:1-21.
8. Kimchi-Sarfaty C, Oh JM, Kim IW, Sauna ZE, Calcagno AM, Ambudkar SV, Gottesman MM. 2007. A "silent" polymorphism in the MDR1 gene changes substrate specificity. Science 315:525-528.
9. Komar AA, Lesnik T, Reiss C. 1999. Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation. FEBS Lett 462:387-391.
10. Lee PS, Lee KH. 2005. Engineering HlyA hypersecretion in Escherichia coli based on proteomic and microarray analyses. Biotechnol Bioeng 89:195-205.
11. Li YY, Chen CX, von Specht BU, Hahn HP. 2002. Cloning and hemolysin mediated secretory expression of a codon-optimized synthetic human interleukin-6 gene in Escherichia coli. Protein Expr Purif 25:437-447.
12. Lilie H, Haehnel W, Rudolph R, Baumann U. 2000. Folding of a synthetic parallel beta-roll protein. FEBS Lett 470:173-177.
13. Mergulhao FJ, Monteiro GA, Larsson G, Sanden AM, Farewell A, Nystrom T, Cabral JM, Taipa MA. 2003. Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB. Appl Microbiol Biotechnol 61:495-501.
14. Pimenta AL, Young J, Holland IB, Blight MA. 1999. Antibody analysis of the localisation, expression and stability of HlyD, the MFP component of the E. coli haemolysin translocator. Mol Gen Genet 261:122-132.
15. Shaw L, Zia R, Lee KH. 2003. Totally asymmetric exclusion process with extended objects: A model for protein synthesis. Phys Rev E 68:021910(1-17).
16. Simmons LC, Yansura DG. 1996. Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nat Biotechnol 14:629-634.
17. Solomovici J, Lesnik T, Reiss C. 1997. Does Escherichia coli optimize the economics of the translation process? J Theor Biol 185:511-521.
18. Stanley P, Koronakis V, Hughes C. 1998. Acylation of Escherichia coli hemolysin: A unique protein lipidation mechanism underlying toxin function. Microbiol Mol Biol Rev 62:309-333.
19. Sugamata Y, Shiba T. 2005. Improved secretory production of recombinant proteins by random mutagenesis of hlyB, an alpha-hemolysin transporter from Escherichia coli. Appl Environ Microbiol 71:656-662.
20. Thanabalu T, Koronakis E, Hughes C, Koronakis V. 1998. Substrate-induced assembly of a contiguous channel for protein export from E. coli: Reversible bridging of an inner-membrane translocase to an outer membrane exit pore. EMBO J 17:6487-6496.
21. Thomas WD, Jr., Wagner SP, Welch RA. 1992. A heterologous membrane protein domain fused to the C-terminal ATP-binding domain of HlyB can export Escherichia coli hemolysin. J Bacteriol 174:6771-6779.
22. Villaverde A, CarrioMM. 2003. Protein aggregation in recombinant bacteria: Biological role of inclusion bodies. Biotechnol Lett 25: 1385-1395.
23. Walsh G. 2006. Biopharmaceutical benchmarks - 2006. Nat Biotechnol 24:769-776.
24. Wiese KC, Glen E, Vasudevan A. 2005. jViz.Rna - A Java Tool for RNA Secondary Structure Visualization. IEEE Trans NanoBioscience 4:212-218.
25. Williams DP, Regier D, Akiyoshi D, Genbauffe F, Murphy JR. 1988. Design, synthesis and expression of a human interleukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes. Nucleic Acids Res 16:10453-10467.
26. Zhang G, Brokx S, Weiner JH. 2006. Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nat Biotechnol 24:100-104.
27. Zuker M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406-3415.