Two-photon FLIM-FRET microscopy for protein localization

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 5323, Issue , 2004, Pages 431-439

  Chen, Ye ^a   Periasamy, Ammasi ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Acceptor photobleaching; C EBP protein; Dimerization; FLIM; FLIM FRET; FRET; Two photon

Indexed keywords

CELLS; DIMERS; ENERGY TRANSFER; PHOTONS; PROBES; PROTEINS; TISSUE;

ACCEPTOR PHOTOBLEACHING; EXPONENTIAL DECAY; FLUORPHORES; LIGHT EXCITATION;

MICROSCOPIC EXAMINATION;

EID: 5644272773     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.538312     Document Type: Conference Paper

References (22)

1. R.B. Sekar and A. Periasamy, "Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localization". J. Cell Biol, 160:629-633.
2. R.M. Clegg, "Fluorescence resonance energy transfer". In Fluorescence Imaging Spectroscopy and Microscopy (X. F. Wang and B. Herman, Eds.), Chemical Analysis Series Vol. 137, pp 179-251. John Wiley & Sons, New York. 1996.
3. R.N. Day, T.C. Voss, J.F. Enwright III, C.F. Booker, A. Periasamy and F. Schaufels, "Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBPá) in the living pituitary cell nucleus". Mol. Endo. 17:333-345, 2003.
4. A.K. Kenworthy, N. Petranova, and M. Edidin, "High-resolution FRET microscopy of cholera toxin Bsubunit and GPI-anchored proteins in cell plasma membranes", Mol. Biol. Cell, 11:1645-1655, 2000.
5. A. Periasamy, M. Elangovan, H. Wallrabe, J.N. Demas, M. Barroso, D.L. Brautigan and R.N. Day, "Wide-field, Confocal, two-photon and Lifetime Energy Transfer Imaging Microscopy". In: Methods in Cellular Imaging, Ed. A. Periasamy, pp 295-308, Oxford University Press, New York, 2001.
6. J.R. Lakowicz, Principles of Fluorescence Spectroscopy. 2 Ed., Plenum Press, New York, 1999.
7. Y. Chen and A. Periasamy, "Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization". Microsc. Res. and Tech. 63:72-80, 2004.
8. Y. Chen, J.D. Mills and A. Periasamy, "Protein interactions in cells and tissues using FLIM and FRET". Differentiation. 71:528-541, 2003.
9. D.V. O'Connor and D. Phillips, Time-Correlated Single Photon Counting. Academic Press, London, 1984.
10. A. Periasamy, P. Wodnicki, X.F. Wang, S. Kwon, G.W. Gordon and B. Herman, "Time resolved fluorescence lifetime imaging microscopy using picosecond pulsed tunable dye laser system". Rev. Sci. Instrum. 67:3722-3731, 1996.
11. G. Marriott, R.M Clegg, J. Arndt-Jovin and T.M. Jovin, "Time resolved imaging microscopy: phosphorescence and delayed fluorescence imaging". Biophys. J. 60:1374-1387, 1991.
12. R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, "Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera". J. Biomed. Opt. 8:362-367, 2003.
13. T. Förster, "Delocalized excitation and excitation transfer". In Modern Quantum Chemistry (O. Sinanoglu, Ed.), Vol. 3, pp. 93-137. Academic Press, New York, 1965.
14. L. Stryer, "Fluorescence energy transfer as a spectroscopic ruler". Annu. Rev. Biochem. 47:819-846, 1978.
15. R.N. Day, "Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy". Mol. Endo. 12:1410-1419, 1998.
16. R. Heim and R.Y. Tsien, "Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer". Curr. Biol. 6:178-182, 1996.
17. H. Wallrabe, M. Elangovan, A. Burchard, A. Periasamy and M. Barroso, "Confocal FRET microscopy to measure clustering of receptor-ligand complexes in endocytic membranes". Biophysical J. 85:559-571, 2003.
18. M. Goppert-Mayer, "Ueber Elementarakte mit zwei Quantenspruengen". Ann. Phys. 9:273, 1931.
19. 2+", Phys. Rev. Lett. 7:229-231, 1961.
20. W. Denk, J.H. Strickler and W.W. Webb, "Two-photon laser scanning fluorescence microscopy". Science 248:73-76, 1990.
21. M. Elangovan, H. Wallrabe, Y. Chen, R.N. Day, M. Barroso and A. Periasamy, "Characterization of one-and two-photon excitation resonance energy transfer microscopy". Methods. 29:58-73, 2003.
22. M. Elangovan, R.N. Day and A. Periasamy, "A novel nanaosecond FRET-FLIM microscopy to quantitate the protein interactions in a single living cell". J Microsc. 1:3-14, 2002.