Dynamic and static fluorescence anisotropy in biological microscopy (rFLIM and emFRET)

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 5323, Issue , 2004, Pages 1-12

  Jovin, Thomas M ^a   Lidke, Diane S ^a   Post, Janine N ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Fluorescence polarization; GFP; Green fluorescent protein; Venus

Indexed keywords

ANISOTROPY; ENERGY TRANSFER; FLUORESCENCE; FREQUENCY DOMAIN ANALYSIS; LIGHT POLARIZATION; PROTEINS; RESONANCE;

GREEN FLUORESCENT PROTEINS; MOLECULAR PARAMETERS; MOLECULAR SUPERSTRUCTURES; PLANE POLARIZED LIGHT;

MICROSCOPIC EXAMINATION;

EID: 5644226253     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.528469     Document Type: Conference Paper

References (25)

1. V. Subramaniam, Q. S. Hanley, A. H. A. Clayton and T. M. Jovin, "Photophysics of green and red fluorescent proteins: some implications for quantitative microscopy," Methods Enzymol. 360, 178-201, 2003.
2. A. H. A. Clayton, Q. S. Hanley, D. J. Arndt-Jovin, V. Subramaniam and T. M. Jovin, "Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM)," Biophys. J. 83, 1631-1649, 2002.
3. D. S. Lidke, P. Nagy, B. G. Barisas, R. Heintzmann, J. N. Post, K. A. Lidke, A. H. A. Clayton, D. J. Arndt-Jovin and T. M. Jovin, "Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)," Biochem. Soc. Trans. 31, 1020-1027, 2003.
4. C. Buehler, C. Y. Dong, P. T. C. So, T. French and E. Gratton, "Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy," Biophys. J. 79, 536-549, 2000.
5. I. Gautier, M. Tramier, C. Durieux, J. Coppey, R. B. Pansu, J. C. Nicolas, K. Kemnitz and M. Coppey-Moisan, "Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins," Biophys. J. 80, 3000-3008, 2001.
6. G. Marriott and I. Parker, Eds. Biophotonics, Part A. Methods Enzymol. 360. San Diego, CA, Academic Press, 2003.
7. J. Siegel, K. Suhling, S. Leveque-Fort, S. E. D. Webb, D. M. Davis, D. Phillips, Y. Sabharwal and P. M. W. French, "Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM): Imaging the rotational mobility of a fluorophore," Rev. Sci. Instrum. 74, 2003.
8. M. Tramier, T. Piolot, I. Gautier, V. Mignotte, J. Coppey, K. Kemnitz, C. Durieux and M. Coppey-Moisan, "Homo-FRET versus hetero-FRET to probe homodimers in living cells," Methods Enzymol. 360, 580-597, 2003.
9. Y. Yan and G. Marriott, "Fluorescence resonance energy transfer imaging microscopy and fluorescence polarization imaging microscopy," Methods Enzymol. 360, 561-580, 2003.
10. A. Squire, P. J. Verveer, O. Rocks and P. I. H. Bastiaens, "Red-edge anisotropy microscopy enables dynamic imaging of homo-FRET between green fluorescent proteins in cells," J. Struct. Biol. 145, in press, 2003.
11. C. E. Bigelow, D. L. Conover and T. H. Foster, "Confocal fluorescence spectroscopy and anisotropy imaging system," Opt. Lett. 28, 695-697, 2003.
12. A. Volkmer, V. Subramaniam, D. J. Birch and T. M. Jovin, "One- and two-photon excited fluorescence lifetimes and anisotropy decays of green fluorescent proteins," Biophys. J. 78, 1589-1598, 2000.
13. E. Jares-Erijman and T. M. Jovin, "Determination of DNA helical handedness by fluorescence resonance energy transfer," J. Mol. Biol. 257, 597-617, 1996.
14. E. A. Jares-Erijman and T. M. Jovin, "Fret imaging," Nat. Biotechnol. 21, 1387-1395, 2003.
15. G. H. Fredrickson, "Concentration depolarization of fluorescence in the presence of molecular rotation," J. Chem. Phys. 88, 5291-5299, 1988.
16. T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba and A. Miyawaki, "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications," Nat. Biotechnol. 20, 87-90, 2002.
17. D. A. Zacharias, J. D. Violin, A. C. Newton and R. Y. Tsien, "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells," Science 296, 913-916, 2002.
18. G. J. Palm, A. Zdanov, G. A. Gaitanaris, R. Stauber, G. N. Pavlakis and A. Wlodawer, "The structural basis for spectral variations in green fluorescent protein," Nat. Struct. Biol. 4, 361-365, 1997.
19. F. I. Rossell and S. G. Boxer, "Polarized absorption spectra of green fluorescent protein single crystals: transition dipole moment directions," Biochemistry 42, 177-183, 2003.
20. I. Z. Steinberg, "Long-range nonradiative transfer of electronic excitation energy in proteins and polypeptides," Annu. Rev. Biochem. 40, 83-114, 1971.
21. A. A. Heikal, S. T. Hess and W. W. Webb, "Multiphoton molecular spectroscopy and excited state dynamics of enhanced green fluorescent protein," Chem. Phys. 274, 37-55, 2001.
22. S. R. Adams, R. E. Campbell, L. A. Gross, B. R. Martin, G. K. Walkup, Y. Yao, J. Llopis and R. Y. Tsien, "New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications," J. Am. Chem. Soc. 124, 6063-6076, 2002.
23. J. N. Forkey, M. E. Quinlan, M. A. Shaw, J. E. T. Corne and Y. E. Goldman, "Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization," Nature 422, 399-404, 2003.
24. H. Wallrabe, M. Elangovan, B. A., A. Periasamy and M. Barroso, "Confocal FRET microscopy to measure clustering of ligand-receptor complexes in endocytic membranes," Biophys. J. 85, 559-571, 2003.
25. P. Sharma, R. Varma, R. C. Sarasij, Ira, K. Gousset, G. Krishnamoorthy, M. Rao and S. Mayor, "Nanoscale organization of multiple GPI-anchored proteins in living cell membranes," Cell 116, 577-589, 2004.