Coagulanolide, a withanolide from Withania coagulans fruits and antihyperglycemic activity

Bioorganic and Medicinal Chemistry Letters

Volumn 18, Issue 24, 2008, Pages 6534-6537

  Maurya, Rakesh ^a   Akanksha, ^a   Jayendra, ^a   Singh, Amar B ^a   Srivastava, Arvind K ^a  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

Author keywords

Antihyperglycemic activity; db db mice; Metformin; SLM; STZ; Withania coagulans; Withanolides

Indexed keywords

COAGULANOLIDE; COAGULIN L; FENOFIBRATE; GLUCOSE; HIGH DENSITY LIPOPROTEIN CHOLESTEROL; LOW DENSITY LIPOPROTEIN CHOLESTEROL; METFORMIN; STREPTOZOCIN; TRIACYLGLYCEROL; UNCLASSIFIED DRUG; VERY LOW DENSITY LIPOPROTEIN CHOLESTEROL; WITHANOLIDE; WITHANOLIDE F;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CHOLESTEROL BLOOD LEVEL; CONTROLLED STUDY; DRUG ISOLATION; DRUG STRUCTURE; FRUIT; GLUCOSE BLOOD LEVEL; LIPOPROTEIN BLOOD LEVEL; MALE; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; ORAL GLUCOSE TOLERANCE TEST; PHYTOCHEMISTRY; PROTON NUCLEAR MAGNETIC RESONANCE; RAT; STREPTOZOCIN DIABETES; TRIACYLGLYCEROL BLOOD LEVEL; WITHANIA; WITHANIA COAGULANS;

ANIMALS; CHEMISTRY, PHARMACEUTICAL; DIABETES MELLITUS, EXPERIMENTAL; HYPERGLYCEMIA; HYPOGLYCEMIC AGENTS; MAGNETIC RESONANCE SPECTROSCOPY; MALE; METFORMIN; MICE; MICE, INBRED C57BL; PLANT EXTRACTS; RATS; RATS, SPRAGUE-DAWLEY; WITHANIA; WITHANOLIDES;

MUS; RATTUS; WITHANIA COAGULANS;

EID: 56249102130     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2008.10.050     Document Type: Article

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24. Type 2 diabetes model, that is, C57BL/KsJ-db/db mice. Hyperglycemic animals were divided into four groups having five animals each. Animals of one group were regarded as control group (orally administered 1% gum acacia) and other groups were treated as experimental groups (treated with suspension of the desired test substances at a dose of 50 mg/kg body weight). The treatment was continued for 10 consecutive days. Animals were dosed daily at a fixed time (10.00-11.00 a.m.) All animals had free access to fresh water and normal diet. Blood glucose profile of each animal was measured by glucometer using glucostrips (Boehringer Mannheim). An oral glucose tolerance test (OGTT) of each individual was performed on day 10 after an overnight fast (10 h). Blood was sampled from the tail vein at time 0 min (baseline), followed by 30, 60, 90 and 120 min after an oral glucose load of 3.0 g/kg of body weight. Quantitative glucose tolerance of each animal was calculated by area under curve (AUC) method using Prism Software. Comparing the AUC of experimental and control groups determined the percentage antihyperglycemic activity.