Stimulated emission depletion and fluorescence correlation spectroscopy of a branched quadrupolar chromophore

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7030, Issue , 2008, Pages

  Armoogum, D A ^a   Marsh, R J ^a   Nicolaou, N ^a   Mongin, O ^b   Blanchard Desce, M ^b   Bain, A J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b CNRS   (France)

Author keywords

Fluorescence correlation spectroscopy; Non radiative relaxation; Single molecule; Stimulated emission depletion

Indexed keywords

CHROMOPHORES; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; LIGHT EMISSION; LUMINESCENCE; MOLECULAR SPECTROSCOPY; MOLECULES; QUENCHING; STIMULATED EMISSION;

EMISSION CROSS-SECTIONS; FLUORESCENCE CORRELATION SPECTROSCOPIES; FLUORESCENCE CORRELATION SPECTROSCOPY; INTERSYSTEM CROSSINGS; NON-RADIATIVE RELAXATION; RADIATIVE RELAXATIONS; SINGLE MOLECULE; SINGLE MOLECULE FLUORESCENCES; SINGLE MOLECULES; STIMULATED EMISSION DEPLETION; STIMULATED EMISSION DEPLETIONS; TRIPLET STATES;

EMISSION SPECTROSCOPY;

EID: 56249085967     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.794558     Document Type: Conference Paper

References (20)

1. Ishijima, A. and Yanagida T., "Single Molecule Nanobioscience", Trends in Biochem. Sci. 26 (7), 438-444 (2001).
2. Wölcke, J. and Ullmann, D., "Miniaturized HTS technologies - uHTS", Drug Discovery Today 6 (12), 637-646 (2001).
3. Rigler, R. and Seela, F., "DNA-Sequencing at the Single Molecule Level", J. Biotech. 86 (3), 161 (2001).
4. Vargas, F., Hollricher, O., Marti, O., de Schaetzen, G. and Tarrach, G., "Influence of protective layers on the blinking of fluorescent single molecules observed by confocal microscopy and scanning near field optical microscopy", J. Chem. Phys. 117 (2), 866-871 (2002).
5. Balasubramanian, S., Klenerman, D., Barnes, C. L. and Osborne, M. A., PCT patent no. WO00/06770 (10th February 2000).
6. Osborne, M. A., Barnes, C. L., Balasubramanian, S. and Klenerman, D., "Probing DNA Surface Attachment and Local Environment Using Single Molecule Spectroscopy", J.Phys. Chem. B. 105 (15), 3120-3126 (2001).
7. Klar, T. A., Jakobs, S., Dyba, M., Egner, A. and Hell, S. W., "Fluorescence Microscopy with Diffraction Resolution Barrier Broken by Stimulated Emission", Proc. Nat. Acad. Sci. 97 (15), 8206-8210 (2000).
8. Marsh, R. J., Bain, A. J. and Osborne, M. A., "Control of single molecule fluorescence dynamics by stimulated emission depletion", Proc. SPIE 5222, 78-86 (2003).
9. Katan, C., Tretiak, S., Werts, M. H. V., Bain, A. J., Marsh, R. J., Leonczek, N., Nicolaou, N., Badaeva, E., Mongin, O. and Blanchard-Desce, M., "Two Photon Transitions in Quadrupolar and Branched Chromophores: Experiment and Theory", J. Phys. Chem. B 111, 9468-9483(2007).
10. Marsh, R. J., Leonczek, N., Armoogum, D. A., Porres, L., Mongin, O., Blanchard-Desce, M. and Bain, A. J., "Stimulated emission depletion dynamics in push-push polyenes", Proc. SPIE 5510, 117-128 (2004).
11. Marsh, R. J., Armoogum, D. A. and Bain, A. J., "Stimulated Emission Depletion of Two-Photon Excited States", Chem. Phys. Lett. 366, 398-405 (2002).
12. Armoogum, D. A., Marsh, R., J. and Bain, A. J., "Polarised Stimulated Emission Depletion of Two-Photon Excited States", Proc SPIE 5222, 34-44 (2004).
13. Dittrich, P. S. and Schwille, P., "Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation", Appl. Phys. B. 73 829-837 (2001).
14. Schwille, P., Kummer, S., Heikal, A. A., Moerner, W. E. and Webb, W. W., "Fluorescence Correlation Spectroscopy Reveals Fast Optical Excitation-Driven Intramolecular Dynamics of Yellow Fluorescent Proteins", Proc. Nat. Acad. Sci. U.S.A. 97, 151-156 (2000).
15. Widengren, J., Rigler, R. Mets, U., "Triplet-State Monitoring by Fluorescence Correlation Spectroscopy", J. Fluorescence 4, 255-258 (1994).
16. Widengren, J., Mets, U. and Rigler, R., "Fluorescence Correlation Spectroscopy of Triplet States in solution: a theoretical and experimental study", J. Phys. Chem. 99, 13368-13379 (1995).
17. Widengren, J., Mets, U. and Rigler, R., "Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy", Chem. Phys. 250, 171-186 (1999).
18. Krichevsky, O. and Bonnet, G., "Fluorescence Correlation Spectroscopy: the technique and its applications", Rep. Prog. Phys. 65, 251-297 (2002).
19. Visser, N. V., Hink, M. A., van Hoek, A. and Visser, A. J. W. G., "Comparison Between Fluorescence Correlation Spectroscopy and Time-Resolved Fluorescence Anisotropy as Illustrated with a Fluorescent Dextran Conjugate", J. Fluorescence 9 (3), 251-255 (1999).
20. Magde, D., Elson, E. L. and Webb, W. W., "Fluorescence Correlation Spectroscopy. II. An Experimental Realisation", Biopolymers 13 (1), 29-61 (1974).