Role of Fyn and PI3K in H2O2-induced inhibition of apical Cl-/OH- exchange activity in human intestinal epithelial cells

Biochemical Journal

Volumn 416, Issue 1, 2008, Pages 99-108

  Saksena, Seema ^a   Gill, Ravinder K ^a   Tyagi, Sangeeta ^a   Alrefai, Waddah A ^a   Ramaswamy, Krishnamurthy ^a   Dudeja, Pradeep K ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Chloride absorption; Down regulated in adenoma (DRA); Human intestine; Phosphoinositide 3 kinase (PI3K); Protein kinase C (PKC); Putative anion transporter 1 (PAT1)

Indexed keywords

CHLORIDE ABSORPTION; DOWN-REGULATED IN ADENOMA (DRA); HUMAN INTESTINE; PHOSPHOINOSITIDE 3-KINASE (PI3K); PROTEIN KINASE C (PKC); PUTATIVE ANION TRANSPORTER 1 (PAT1);

ABSORPTION; BIOMATERIALS; CALCIUM; CELL CULTURE; CELLS; MICROSTRIP ANTENNAS; MICROWAVE ANTENNAS; OXYGEN; SODIUM CHLORIDE; TUMORS;

NEGATIVE IONS;

CARRIER PROTEINS AND BINDING PROTEINS; HYDROGEN PEROXIDE; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOLIPASE C GAMMA1; PROTEIN KINASE C ALPHA; PROTEIN KINASE FYN; PROTEIN P85; REACTIVE OXYGEN METABOLITE; SLC26A3 PROTEIN; SLC26A6 PROTEIN; UNCLASSIFIED DRUG; AMINO ACID TRANSPORTER; ANTIPORTER; BICARBONATE CHLORIDE ANTIPORTER; CHLORIDE BASE EXCHANGER; CHLORIDE-BASE EXCHANGER; COTRANSPORTER; FYN PROTEIN, HUMAN; PHOSPHOLIPASE C GAMMA; SLC26A3 PROTEIN, HUMAN; SLC36A1 PROTEIN, HUMAN;

ANION EXCHANGE; APICAL MEMBRANE; ARTICLE; CALCIUM CELL LEVEL; CELL STRAIN CACO 2; CELL SURFACE; CHLORIDE HYDROXIDE EXCHANGE; CHLORIDE TRANSPORT; CONTROLLED STUDY; DIARRHEA; HUMAN; HUMAN CELL; INFLAMMATION; INTESTINE EPITHELIUM CELL; NUCLEOTIDE SEQUENCE; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; CYTOLOGY; DRUG ANTAGONISM; INTESTINE MUCOSA; METABOLISM; PHYSIOLOGY;

ORYCTOLAGUS CUNICULUS; RATTUS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMINO ACID TRANSPORT SYSTEMS; ANTIPORTERS; CACO-2 CELLS; CHLORIDE-BICARBONATE ANTIPORTERS; HUMANS; HYDROGEN PEROXIDE; INTESTINAL MUCOSA; PHOSPHOLIPASE C GAMMA; PROTEIN KINASE C-ALPHA; PROTO-ONCOGENE PROTEINS C-FYN; SYMPORTERS;

EID: 56049092758     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20070960     Document Type: Article

References (43)

1. Halliwell, B. (1994) Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344, 721-724
2. Halliwell, B., Zhao, K. and Whiteman, M. (2000) The gastrointestinal tract: a major site of antioxidant action? Free Radical Res. 33, 819-830
3. Harris, M. L., Schiller, H. J., Reilly, P. M., Donowitz, M., Grisham, M. B. and Bulkley, G. B. (1992) Free radicals and other reactive oxygen metabolites in inflammatory bowel disease: cause, consequence or epiphenomenon? Pharmacol. Ther. 53, 375-408
4. Eiserich, J. P., Cross, C. E., Jones, A. D., Halliwell, B. and van der Vliet, A. (1996) Formation of nitrating and chlorinating species by reaction of nitrite with hypochlorous acid. A novel mechanism for nitric oxide-mediated protein modification. J. Biol. Chem. 271, 19199-19208
5. Karayalcin, S. S., Sturbaum, C. W., Wachsman, J. T., Cha, J. H. and Powell, D. W. (1990) Hydrogen peroxide stimulates rat colonic prostaglandin production and alters electrolyte transport. J. Clin. Invest. 86, 60-68
6. Mayol, J. M., Adame-Navarrete, Y., Alarma-Estrany, P., Molina-Roldan, E., Huete-Toral, F. and Fernandez-Represa, J. A. (2006) Luminal oxidants selectively modulate electrogenic ion transport in rat colon. World J. Gastroenterol. 12, 5523-5527
7. Nguyen, T. D. and Canada, A. T. (1994) Modulation of human colonic T84 cell secretion by hydrogen peroxide. Biochem. Pharmacol. 47, 403-410
8. - secretion across colonic epithelial cells. Am. J. Physiol. 275, C1313-C1322
9. 2+ overload in NRVM involves ERK1/2 MAP kinases: role for an NHE-1-dependent pathway. Am. J. Physiol. Heart Circ. Physiol. 283, H598-H605
10. Ortiz, P. A. and Garvin, J. L. (2002) Superoxide stimulates NaCl absorption by the thick ascending limb. Am. J. Physiol. Renal Physiol. 283, F957-F962
11. Bae, Y. S., Kang, S. W., Seo, M. S., Baines, I. C., Tekle, E., Chock, P. B. and Rhee, S. G. (1997) Epidermal growth factor (EGF)-induced generation of hydrogen peroxide. Role in EGF receptor-mediated tyrosine phosphorylation. J. Biol. Chem. 272, 217-221
12. Hardwick, J. S. and Sefton, B. M. (1997) The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505. J. Biol. Chem. 272, 25429-25432
13. 2 for platelet-derived growth factor signal transduction. Science 270, 296-299
14. Natarajan, V., Taher, M. M., Roehm, B., Parinandi, N. L., Schmid, H. H., Kiss, Z. and Garcia, J. G. (1993) Activation of endothelial cell phospholipase D by hydrogen peroxide and fatty acid hydroperoxide. J. Biol. Chem. 268, 930-937
15. Kiss, Z. and Anderson, W. H. (1994) Hydrogen peroxide regulates phospholipase D-mediated hydrolysis of phosphatidylethanolamine and phosphatidylcholine by different mechanisms in NIH 3T3 fibroblasts. Arch. Biochem. Biophys. 311, 430-436
16. 2. Proc. Natl. Acad. Sci. U.S.A. 94, 11233-11237
17. Wang, X. T., McCullough, K. D., Wang, X. J., Carpenter, G. and Holbrook, N. J. (2001) Oxidative stress-induced phospholipase C-γ 1 activation enhances cell survival. J. Biol. Chem. 276, 28364-28371
18. Olsnes, S., Tonnessen, T. I., Ludt, J. and Sandvig, K. (1987) Effect of intracellular pH on the rate of chloride uptake and efflux in different mammalian cell lines. Biochemistry 26, 2778-2785
19. - exchange activity in Caco-2 cells by serotonin. J. Biol. Chem. 280, 11859-11868
20. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
21. + exchange activity via 5-HT4 receptors and activation of PKCα in human intestinal epithelial cells. Gastroenterology 128, 962-974
22. - exchange following infection with enteropathogenic E. coli. J. Clin. Invest. 117, 428-437
23. Rao, R., Baker, R. D. and Baker, S. S. (1999) Inhibition of oxidant-induced barrier disruption and protein tyrosine phosphorylation in Caco-2 cell monolayers by epidermal growth factor. Biochem. Pharmacol. 57, 685-695
24. Rao, R. K., Baker, R. D., Baker, S. S., Gupta, A. and Holycross, M. (1997) Oxidant-induced disruption of intestinal epithelial barrier function: role of protein tyrosine phosphorylation. Am. J. Physiol. 273, G812-G823
25. Lee, S. F., Huang, Y. T., Wu, W. S. and Lin, J. K. (1996) Induction of c-jun protooncogene expression by hydrogen peroxide through hydroxyl radical generation and p60SRC tyrosine kinase activation. Free Radical Biol. Med. 21, 437-448
26. Abe, J., Okuda, M., Huang, Q., Yoshizumi, M. and Berk, B. C. (2000) Reactive oxygen species activate p90 ribosomal S6 kinase via Fyn and Ras. J. Biol. Chem. 275, 1739-1748
27. Gentili, C., Morelli, S. and Russo De Boland, A. (2002) Involvement of PI3-kinase and its association with c-Src in PTH-stimulated rat enterocytes. J. Cell. Biochem. 86, 773-783
28. Susa, M., Rohner, D. and Bichsel, S. (1996) Differences in binding of PI 3-kinase to the src-homology domains 2 and 3 of p56 lck and p59 fyn tyrosine kinases. Biochem. Biophys. Res. Commun. 220, 729-734
29. Khare, S., Bolt, M. J., Wali, R. K., Skarosi, S. F., Roy, H. K., Niedziela, S., Scaglione-Sewell, B., Aquino, B., Abraham, C., Sitrin, M. D. et al. (1997) 1,25 dihydroxyvitamin D3 stimulates phospholipase C-γ in rat colonocytes: role of c-Src in PLC-γ activation. J. Clin. Invest. 99, 1831-1841
30. Rameh, L. E., Rhee, S. G., Spokes, K., Kazlauskas, A., Cantley, L. C. and Cantley, L. G. (1998) Phosphoinositide 3-kinase regulates phospholipase Cγ-mediated calcium signaling. J. Biol. Chem. 273, 23750-23757
31. Banan, A., Fields, J. Z., Farhadi, A., Talmage, D. A., Zhang, L. and Keshavarzian, A. (2002) Activation of δ-isoform of protein kinase C is required for oxidant-induced disruption of both the microtubule cytoskeleton and permeability barrier of intestinal epithelia. J. Pharmacol. Exp. Ther. 303, 17-28
32. +/ glucose cotransporter (SGLT1) is involved in trafficking to the plasma membrane. FEBS Lett. 377, 181-184
33. Prince, L. S., Workman, Jr, R. B. and Marchase, R. B. (1994) Rapid endocytosis of the cystic fibrosis transmembrane conductance regulator chloride channel. Proc. Natl. Acad. Sci. U.S.A. 91, 5192-5196
34. Mount, D. B. and Romero, M. F. (2004) The SLC26 gene family of multifunctional anion exchangers. Pflugers Arch. 447, 710-721
35. - exchanger in the small intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 282, G573-G579
36. Karnitz, L. M., Sutor, S. L. and Abraham, R. T. (1994) The Src-family kinase, Fyn, regulates the activation of phosphatidylinositol 3-kinase in an interleukin 2-responsive T cell line. J. Exp. Med. 179, 1799-1808
37. Polk, D. B. (1998) Epidermal growth factor receptor-stimulated intestinal epithelial cell migration requires phospholipase C activity. Gastroenterology 114, 493-502
38. Chen, C. C., Liau, C. S. and Lee, Y. T. (1996) Tumor necrosis factor-α, platelet-activating factor, and hydrogen peroxide activate protein kinase C subtypes α and ε in human saphenous vein endothelial cells. J. Cardiovasc. Pharmacol. 28, 240-244
39. - exchange activity in Caco-2 cells by phorbol esters is mediated by PKCε. Am. J. Physiol. Cell Physiol. 283, C1492-C1500
40. - exchange activity in Caco2 cells. Dig. Dis. Sci. 52, 1270-1278
41. Basuroy, S., Sheth, P., Kuppuswamy, D., Balasubramanian, S., Ray, R. M. and Rao, R. K. (2003) Expression of kinase-inactive c-Src delays oxidative stress-induced disassembly and accelerates calcium-mediated reassembly of tight junctions in the Caco-2 cell monolayer. J. Biol. Chem. 278, 11916-11924
42. + exchanger NHE3 via mechanism involving phosphatidylinositol 3-kinase. J. Biol. Chem. 275, 8133-8142
43. Obenauer, J. C., Cantley, L. C. and Yaffe, M. B. (2003) Scansite 2.0: proteome-wide prediction of cell signaling interactions using short sequence motifs. Nucleic Acids Res. 31, 3635-3641