How DASPMI reveals mitochondrial membrane potential: Fluorescence decay kinetics and steady-state anisotropy in living cells

Biophysical Journal

Volumn 95, Issue 8, 2008, Pages 4068-4076

  Ramadass, Radhan ^a   Bereiter Hahn, Jürgen ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2 (4 (DIMETHYLAMINO)STYRYL) 1 METHYLPYRIDINIUM; 2-(4-(DIMETHYLAMINO)STYRYL)-1-METHYLPYRIDINIUM; PYRIDINIUM DERIVATIVE;

ANIMAL; ANISOTROPY; ARTICLE; CELL LINE; CELL SURVIVAL; DRUG EFFECT; FLUORESCENCE; KINETICS; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL MEMBRANE POTENTIAL; TIME; XENOPUS;

ANIMALS; ANISOTROPY; CELL LINE; CELL SURVIVAL; FLUORESCENCE; KINETICS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIAL MEMBRANES; PYRIDINIUM COMPOUNDS; TIME FACTORS; XENOPUS;

EID: 56049092327     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.108.135079     Document Type: Article

References (23)

1. Chen, L. B. 1988. Mitochondrial membrane potential in living cells. Annu. Rev. Cell Biol. 4:155-181.
2. Bereiter-Hahn, J. 1990. Behavior of mitochondria in the living cell. Int. Rev. Cytol. 122:1-63.
3. Balaban, R. S., S. Nemoto, and T. Finkel. 2005. Mitochondria, oxidants, and aging. Cell. 120:483-495.
4. Lambert, A. J., and M. D. Brand. 2007. Research on mitochondria and ageing. Aging Cell. 6:417-420.
5. Orrenius, S. 2007. Reactive oxygen species in mitochondria-mediated cell death. Drug Metab. Rev. 39:443-455.
6. Haugland, R. P. 2002. Handbook of Fluorescent Probes and Research Products. Molecular Probes, Eugene, OR. 74-76, 86-96, 830-837, 867.
7. Bereiter-Hahn, J. 1976. Dimethylaminostyrylmethylpyridiniumiodine (DASPMI) as a fluorescent probe for mitochondria in situ. Biochim. Biophys. Acta. 423:1-14.
8. Bereiter-Hahn, J., K. H. Seipel, M. Vöth, and J. S. Ploem. 1983. Fluorometry of mitochondria in cells vitally stained with DASPMI or rhodamine 6 GO. Cell Biochem. Funct. 1:147-155.
9. Mewes, W. H., and J. Rafael. 1981. The 2-(dimethylaminostyryl)-1- methylpyridinium cation as indicator of the mitochondrial membrane potential. FEBS Lett. 131:7-10.
10. Ramadass, R., and J. Bereiter-Hahn. 2007. Photophysical properties of DASPMI as revealed by spectrally resolved fluorescence decays. J. Phys. Chem. B. 111:7681-7690.
11. Schlage, W., C. Kuhn, and J. Bereiter-Hahn. 1981. Established Xenopus tadpole heart endothelium (XTH) cells exhibiting selected properties of primary cells. Eur. J. Cell Biol. 24:342. (Abstr.)
12. Jacob, M. C., M. Favre, and J. C. Bensa. 1991. Membrane cell permeabilization with saponin and multiparametric analysis by flow cytometry. Cytometry. 12:550-558.
13. Saks, V. A., V. I. Velsler, A. V. Kuznetsov, L. Kay, P. Sikk, T. Tiivel, L. Tranqui, J. Olivares, K. Winkler, F. Wiedemann, and W. S. Kunz. 1998. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol. Cell. Biochem. 184:81-100.
14. Ramadass, R., D. Becker, M. Jendrach, and J. Bereiter-Hahn. 2007. Spectrally and spatially resolved fluorescence lifetime imaging in living cells: TRPV4-microfilament interactions. Arch. Biochem. Biophys. 463:27-36.
15. O'Shea, S. P., S. Feuerstein-Thelen, and A. Azzi. 1984. Membrane-potential-dependent changes of the lipid microviscosity of mitochondria and phospholipid vesicles. Biochem. J. 220:795-801.
16. Kumar, C. V., R. S. Turner, and E. H. Asuncion. 1993. Groove binding of a styrylcyanine dye to the DNA double helix: the salt effect. J. Photochem. Photobiol. Chem. 74:231-238.
17. Bereiter-Hahn, J., and M. Vöth. 1998. Do single mitochondria contain zones with different membrane potential? Exp. Biol. Online. 3:1-13.
18. Smiley, S. T., M. Reers, C. Mottolahartshorn, M. Lin, A. Chen, T. W. Smith, G. D. Steele, and L. B. Chen. 1991. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. USA. 88:3671-3675.
19. Loew, L. M., S. Scully, L. Simpson, and A. S. Waggoner. 1979. Evidence for a charge-shift electrochromic mechanism in a probe of membrane potential. Nature. 281:497-499.
20. Loew, L. M., and L. L. Simpson. 1981. Charge-shift probes of membrane potential: a probable electrochromic mechanism for p-aminostyrylpyridinium probes on a hemispherical lipid bilayer. Biophys. J. 34:353-365.
21. Loew, L. M., G. W. Bonneville, and J. Surow. 1978. Charge shift optical probes of membrane potential. Theory. Biochemistry. 17:4065-4071.
22. Loew, L. M. 1996. Potentiometric dyes: imaging electrical activity of cell membranes. Pure Appl. Chem. 68:1405-1409.
23. Loew, L. M., L. Simpson, A. Hassner, and V. Alexanian. 1979. An unexpected blue shift caused by differential solvation of a chromophore oriented in a lipid bilayer. J. Am. Chem. Soc. 101:5439-5440.