Identification of a novel genomic island specific to hospital-acquired clonal complex 17 Enterococcus faecium isolates

Applied and Environmental Microbiology

Volumn 74, Issue 22, 2008, Pages 7094-7097

  Heikens, Esther ^a   Van Schaik, Willem ^a   Leavis, Helen L ^a   Bonten, Marc J M ^a   Willems, Rob J L ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CARBOHYDRATES; COMPETITION; GENES; ORGANIC COMPOUNDS;

CLONAL COMPLEXES; COMPETITIVE ADVANTAGES; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS FAECIUM STRAINS; GENOMIC ISLANDS; METABOLIC PATHWAYS; MICROBIOTA; RESISTANCE GENES; VIRULENCE GENES;

HOSPITALS;

BACTERIAL DNA;

BACTERIUM; CLONE; HOSPITAL SECTOR; IDENTIFICATION METHOD; METABOLISM; VIRULENCE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENOME; BACTERIAL INFECTION; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CARBOHYDRATE METABOLISM; ENTEROCOCCUS FAECIUM; GENOMIC ISLAND; HOSPITAL INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; TRANSCRIPTION REGULATION;

CARBOHYDRATE METABOLISM; CROSS INFECTION; ENTEROCOCCUS FAECIUM; GENE ORDER; GENES, BACTERIAL; GENOMIC ISLANDS; GRAM-POSITIVE BACTERIAL INFECTIONS;

ENTEROCOCCUS FAECIUM;

EID: 55949114228     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01378-08     Document Type: Article

References (20)

1. Dobrindt, U., B. Hochhut, U. Hentschel, and J. Hacker. 2004. Genomic islands in pathogenic and environmental microorganisms. Nat. Rev. Microbiol. 2:414-424.
2. Gaballa, A., and J. D. Helmann. 2007. Substrate induction of siderophore transport in Bacillus subtilis mediated by a novel one-component regulator. Mol. Microbiol. 66:164-173.
3. Gallegos, M. T., R. Schleif, A. Bairoch, K. Hofmann, and J. L. Ramos. 1997. AraC/XylS family of transcriptional regulators. Microbiol. Mol. Biol. Rev. 61:393-410.
4. Hacker, J., and E. Carniel. 2001. Ecological fitness, genomic islands and bacterial pathogenicity. A Darwinian view of the evolution of microbes. EMBO Rep. 2:376-381.
5. Hacker, J., and J. B. Kaper. 2000. Pathogenicity islands and the evolution of microbes. Annu. Rev. Microbiol. 54:641-679.
6. Heikens, E., M. J. Bonten, and R. J. Willems. 2007. Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162. J. Bacteriol. 189:8233-8240.
7. Hendrickx, A. P., W. J. Van Wamel, G. Posthuma, M. J. Bonten, and R. J. Willems. 2007. Five genes encoding surface-exposed LPXTG proteins are enriched in hospital-adapted Enterococcus faecium clonal complex 17 isolates. J. Bacteriol. 189:8321-8332.
8. Karlin, S. 2001. Detecting anomalous gene clusters and pathogenicity islands in diverse bacterial genomes. Trends Microbiol. 9:335-343.
9. Karlin, S., and C. Burge. 1995. Dinucleotide relative abundance extremes: a genomic signature. Trends Genet. 11:283-290.
10. Leavis, H. L., J. Top, N. Shankar, K. Borgen, M. J. Bonten, J. D. van Embden, and R. J. L. Willems. 2004. A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity. J. Bacteriol. 186:672-682.
11. Leavis, H. L., M. J. Bonten, and R. J. Willems. 2006. Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance. Curr. Opin. Microbiol. 9:454-460.
12. Leavis, H. L., R. J. L. Willems, J. Top, and M. J. Bonten. 2006. High-level ciprofloxacin resistance from point mutations in gyrA and parC confined to global hospital-adapted clonal lineage CC17 of Enterococcus faecium. J. Clin. Microbiol. 44:1059-1064.
13. Leavis, H. L., R. J. Willems, W. J. Van Wamel, F. H. Schuren, M. P. Caspers, and M. J. Bonten. 2007. Insertion sequence-driven diversification creates a globally dispersed emerging multiresistant subspecies of Enterococcus faecium. PLoS. Pathog. 3:e7.
14. Martin, R. G., and J. L. Rosner. 2001. The AraC transcriptional activators. Curr. Opin. Microbiol. 4:132-137.
15. Sahm, D. F., J. Kissinger, M. S. Gilmore, P. R. Murray, R. Mulder, J. Solliday, and B. Clarke. 1989. In vitro susceptibility studies of vancomycin-resistant Enterococcus faecalis. Antimicrob. Agents Chemother. 33:1588-1591.
16. van Passel, M. W., A. C. M. Luyf, A. H. C. van Kampen, A. Bart, and A. van der Ende. 2005. δρ-Web, an online tool to assess composition similarity of individual nucleic acid sequences. Bioinformatics 21:3053-3055.
17. Van Wamel, W. J. B., A. P. A. Hendrickx, M. J. M. Bonten, J. Top, G. Posthuma, and R. J. L. Willems. 2007. Growth condition-dependent Esp expression by Enterococcus faecium affects initial adherence and biofilm formation. Infect. Immun. 75:924-931.
18. Willems, R. J., W. Homan, J. Top, M. van Santen-Verheuvel, D. Tribe, X. Manzioros, C. Gaillard, C. M. Vandenbroucke-Grauls, E. M. Mascini, E. van Kregten, J. D. Van Embden, and M. J. Bonten. 2001. Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. Lancet 357:853-855.
19. Willems, R. J., J. Top, M. van Santen, D. A. Robinson, T. M. Coque, F. Baquero, H. Grundmann, and M. J. Bonten. 2005. Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg. Infect. Dis. 11:821-828.
20. Zuker, M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31:3406-3415.