Optimizing environmental scanning electron microscopy of spheroidal reaggregated neuronal cultures

Microscopy Research and Technique

Volumn 71, Issue 11, 2008, Pages 792-801

  Uroukov, Ivan S ^a   Patton, David ^a  

^a UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

Author keywords

3D neuronal networks; Artificial brain structure; Biological specimens; Fully hydrated; MEA

Indexed keywords

ELECTROPHYSIOLOGY; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; HYDRATION; NEURAL NETWORKS; NEURONS;

3D NEURONAL NETWORK; ARTIFICIAL BRAIN; ARTIFICIAL BRAIN STRUCTURE; BIOLOGICAL SPECIMENS; BRAIN STRUCTURE; ELECTROPHYSIOLOGICAL RECORDINGS; ENVIRONMENTAL SCANNING; FULLY HYDRATED; MEA; NEURONAL NETWORKS;

SCANNING ELECTRON MICROSCOPY;

WATER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; EMBRYO; ENVIRONMENT; FIBER; FILM; NERVE CELL CULTURE; NONHUMAN; PRESSURE; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SURFACE PROPERTY; TRANSMISSION ELECTRON MICROSCOPY;

EID: 55849136099     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20621     Document Type: Article

References (40)

1. Bollard MW, Xu J, Purcell WM, Griffin JL, Quirk C, Holmes E, Nicholson JK. 2002. Metabolic profiling of the effects of D-galactosamine in liver spheroids using (1)H NMR and MAS-NMR spectroscopy. Chem Res Toxicol 15:1351-1359.
2. Bourges J-L, Gautier G, Delie F, Bejjani RA, Jeanny J-C, Gurney R, BenEzra D, Behar-Cohen FF. 2003. Ocular drug delivery targeting the retina and retinal pigment epithelium using polylactide nanoparticles. Invest Opthalmol Vis Sci 44:3562-3569.
3. Bull L, Uroukov I. 2007. Initial results from the use of learning classifier systems to control in vitro neuronal networks. In: Thierens D, editor. GECOO-2007: Proceedings of the genetic and evolutionary computation conference. New York: ACM Press. pp. 369-376.
4. Cyster LA, Parker KG, Parker TL, Grant DM. 2004. The effect of surface chemistry and nanotopography of titanium nitride (TiN) films on primary hippocampal neurons. Biomaterials 25:97-107.
5. DeLong GR. 1970. Histogenesis of fetal mouse isocortex and hippocampus in reaggregating cell cultures. Dev Biol 22:563-583.
6. Forster H, Fisher J. 1999. The influence of continuous sliding and subsequent surface wear on the friction of articular cartilage. Proc Inst Mech Eng [H] 233:329-345.
7. Goldsmith PC, Berens ME. 1990. Brain cell culture in rotation-mediated aggregates. Methods Neurosci 2:236-263.
8. Graindorge SL, Stachowiak GW. 2000. Changes occurring in the surface morphology of articular cartilage during wear. Wear 241:143-150.
9. Gribble K, Sarafis V, Nailon J, Holford P, Uwins P. 1996. Environmental scanning electron microscopy of the surface of normal and vitrified leaves of Gypsophilia paniculata (Babies Breath) cultured in vitro. Plant Cell Rep 15:771-776.
10. Gross GW. 1979. Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface. IEEE Trans Biomed Eng 26:273-279.
11. Guillory KS, Norman RA. 1999. A 100-channel system for real time detection and storage of extracellular spike waveforms. J Neurosci Methods 91:21-29.
12. Habold C, Dunel-Erb S, Chevalier C, Laurent P, Le Maho Y, Lignot JH. 2003. Observations of the intestinal mucosa using environmental scanning electron microscopy (ESEM); comparison with conventional scanning electron microscopy (CSEM). Micron 34:373-379.
13. Hamburger V, Hamilton HL. 1951. A series of normal stages in the development of the chick embryo. J Morphol 88:49-92.
14. Heuschkel M, Fejtal M, Raggenbass M, Bertrand D, Renaut P. 2002. A three-dimensional multi-electrode array for multisite stimulation and recording in acute brain slices. J Neurosci Methods 114:135-148.
15. Hutcheon GA, Downes S. 1998. Interactions of chondrocytes with methacrylate copolymers. J Mater Sci Mater Med 9:815-818.
16. Jimbo Y, Kawana A, Parodi P, Torre V. 2000. The dynamics of a neuronal culture of dissociated cortical neurons of neonatal rats. Biol Cybern 83:1-20.
17. Joester D, Klein E, Geiger B, Addadi L. 2006. Temperature-sensitive micrometer-thick layers of hyaluronan grafted on microspheres. J Am Chem Soc 128:1119-1124.
18. Kolb D, Müller M. 2004. Light, conventional and environmental scanning electron microscopy of tricomes of Cucurbita pepo subsp. pepo var. styriaca and histochemistry of glandular secretory products. Ann Bot 94:515-526.
19. McKinley KJ, Allison FJ, Scotchford CA, Grant DM, Oliver JM, King JR, Wood JV, Brown PD. 2004. Comparison of environmental scanning electron microscopy with high vacuum scanning electron microscopy as applied to the assessment of cell morphology. J Biomed Mater A 69:359-366.
20. Ma M, Xu J, Purcell WM. 2003. Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture. I. Morphological maturation and kinetics changes of energy metabolism, albumin synthesis and activities of some enzymes. J Cell Biochem 90:1166-1175.
21. Martínez-Alvarez C, Bonelli R, Tudela C, Gato A, Mena J, O'Kane S, Ferguson MWJ. 2000. Bulging medial edge cells and palatial fusion. Int J Dev Biol 44:331-335.
22. Misirli Z, Öner ET, Kirdar B. 2007. Real imaging and size values of Saccharomyces cerevisiae cells with comparable contrast tuning to two environmental scanning electron microscopy modes. Scanning 29:11-19.
23. Moscona A. 1961. Rotation-mediated histogenic aggregation of dissociated cells. Exp Cell Res 22:455-475.
24. Muscariello L, Rosso F, Marino G, Giordano A, Barbarisi M, Cafiero G, Barbarisi A. 2005. A critical overview of ESEM applications in the biological field. J Cell Physiol 205:328-334.
25. Nedela V. 2007. Methods for additive hydration allowing observation of fully hydrated state of wet samples in environmental SEM. Microsc Res Tech 70:95-100.
26. Notenier R, Tonetto A, Da Prato J, Barthélémy R. 2004. SEM versus ESEM for crustacean studies. Microsc Anal 18:29.
27. Pine J. 1980. Recording action potentials from cultured neurons with extracellular microcircuit electrodes. J Neurosci Methods 2:19-31.
28. Priester JH, Horst AM, Van De Werfhorst LC, Saleta JL, Mertes LAK, Holden PA. 2007. Enhanced visualization of microbial biofilms by staining and environmental scanning electron microscopy. J Microbiol Methods 68:577-587.
29. Reid DA, Lott JNA, Attree SM, Fowke LC. 1999. Inhibition of white spruce seeds and somatic embryos: A study of morphological changes in an environmental scanning electron microscope and potassium leakage. In Vitro Cell Dev Biol Plant 35:303-308.
30. Sales KM, Kingston ST, Doyle KM, Purcell WM. 2004. Preliminary characterisation of an in vitro paradigm for the study of the delayed effects of organophosphorus compounds: Hen embryo brain spheroids. Toxicology 195:187-202.
31. Seeds NW. 1971. Biochemical differentiation in reaggregating brain cell culture. Proc Nat Acad Sci USA 68:1858-1861.
32. Shao M, Jiang L, Cong W, Ouyang F. 2002. Analysis of vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy. Sci Chin (Series C) 45:149-158.
33. Sidman RL. 1970. Cell proliferation, migration and interaction in the developing mammalian central nervous system. In: Schmitt FO, editor. The neurosciences second study program. New York: Rockefeller University Press. pp. 100-107.
34. Stokes DJ, Rea SM, Best SM, Bonfield W. 2003. Electron microscopy of mammalian cells in the absence of fixing. Freezing, dehydration, or specimen coating. Scanning 25:181-184.
35. Strafstrom CE, Johnston JM, Weinher JM, Sheppard JR. 1980. Spontaneous neural activity in fetal brain reaggregate cultures. Neuroscience 5:1681-1689.
36. Tai SSW, Tang XM. 2001. Manipulating biological samples for environmental scanning electron microscopy observation. Scanning 23:267-272.
37. Trapp BD, Honneger P, Richelson E, Webster HD. 1979. Morphological differentiation of mechanically dissociated fetal rat brain in aggregating cell cultures. Brain Res 160:117-180.
38. Uroukov IS, Ma M, Bull L, Purcell WM. 2006. Electrophysiological measurements in three-dimensional in vivo-mimetic organotypic cell cultures: Preliminary studies with hen embryo brain spheroids. Neurosci Lett 404:33-38.
39. Wagenaar DA, Pine J, Potter SM. 2004. Effective parameters for stimulation of associated cultures using multi-electrode arrays. J Neurosci Methods 138:27-37.
40. Xu J, Ma M, Purcell WM. 2003. Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture. II. Nitric oxide synthesis and related changes. J Cell Biochem 90:1176-1185.