Methods for additive hydration allowing observation of fully hydrated state of wet samples in environmental SEM

Microscopy Research and Technique

Volumn 70, Issue 2, 2007, Pages 95-100

  Nedéla, Vilém ^a  

^a INSTITUTE OF SCIENTIFIC INSTRUMENTS   (Czech Republic)

Author keywords

Agar; Biological specimens; Environmental SEM; Natural structure

Indexed keywords

ALGAE; HYDRATION; POLYSACCHARIDES; SCANNING ELECTRON MICROSCOPY;

AGAR; BIOLOGICAL SPECIMENS; CRITICAL POINT DRYING; ENVIRONMENTAL SCANNING ELECTRON MICROSCOPES; ENVIRONMENTAL SEM; NATURAL STRUCTURES; PELTIER; SPECIMEN STRUCTURES; WATER VAPOUR; WET SAMPLE;

DEHYDRATION;

ADDITIVE HYDRATION; ARTICLE; CRITICAL POINT DRYING METHOD; ENVIRONMENTAL FACTOR; EVALUATION; HUMAN; HUMAN CELL; HYDRATION; INTERMETHOD COMPARISON; PELTIER COOLED HOLDER; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; STRUCTURE ANALYSIS;

EID: 33847165777     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20390     Document Type: Article

References (11)

1. Cameron RE, Donald AM. 1994. Minimizing sample evaporation in the environmental scanning electron microscope. J Microsc 173:227-237.
2. Danilatos GD. 1988. Foundations of environmental scanning electron microscopy. Adv Electron Electron Phys 71:109-250.
3. Danilatos GD. 1990. Theory of the gaseous detector device in the environmental scanning electron microscopy. Adv Electron Electron Phys 78:1-102.
4. Lane WC. 1970. The environmental control stage. Scan Electron Microsc 1970:43-48.
5. Moncrieff DA, Robinson VNE, Harris LB. 1978. Charge neutralisation of insulating surfaces in the SEM by gas ionisation. J Phys D 11:2315-2325.
6. Neděla V, Maxa J. 2006. Environmental scanning electron microscope AQUASEM II-The design and applications. In: Proceedings of the 10th International Seminar Skalský Dvůr, ISI AS CR Brno, 2006 pp. 55-56.
7. Robinson VNE. 1975a. The elimination of charging artefacts in the SEM. J Phys E 8:638-640.
8. Robinson VNE. 1975b. A wet stage modification to a scanning electron microscope. J Microsc 103:71-77.
9. Romanovský V, Autrata R. 2000. Combined scintillation and ionisation detectors for environmental scanning electron microscopes. In: Proceedings of the 12th EUREM, Czechoslovak Society of Electron Microscopy, 2000, Vol. 3, pp. 241-242.
10. Sanger P, Ritter M, Hohenberg H. 2003. Water reservoirs (matrices) prevent rapid dehydration of native samples in the environmental scanning electron microscope (ESEM). Microsc Microanal 9 (Suppl. 3):494-495.
11. Stokes DJ. 2003. Investigating biological ultrastructure using environmental scanning electron microscopy (ESEM). In: Méndez-Vilas A, editor. Science, technology and education of microscopy: An overview. Badajoz, Spain: Formatex. pp. 564-570.