In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy

Journal of Microscopy

Volumn 232, Issue 2, 2008, Pages 270-275

  Gualda, E J ^a   Filippidis, G ^a   Mari, M ^a,c   Voglis, G ^b   Vlachos, M ^b   Fotakis, C ^a,c   Tavernarakis, N ^b  

^a INSTITUTE OF ELECTRONIC STRUCTURE AND LASER   (Greece)

^b INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

^c UNIVERSITY OF CRETE   (Greece)

Author keywords

Caenorhabditis elegans; Imaging; Microscopy; Neuron degeneration; THG

Indexed keywords

HARMONIC GENERATION; LASER EXCITATION; LASER PULSES;

CAENORHABDITIS ELEGANS; IMAGING MEASUREMENTS; IN-VIVO; IN-VIVO IMAGING; NEURODEGENERATION; NEURON DEGENERATION; REAL- TIME; THG; THIRD-HARMONIC GENERATION IMAGING; THIRD-HARMONIC GENERATION MICROSCOPY;

HARMONIC ANALYSIS;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; IN VIVO STUDY; LASER; MICROSCOPY; NERVE DEGENERATION; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

CAENORHABDITIS ELEGANS;

EID: 55349090327     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.02098.x     Document Type: Article

References (18)

1. Brenner, S 1974) The genetics of Caenorhabditis elegans. Genetics 77, 71 94.
2. Campagnola, P.J. & Loew, L.M. (2003) Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms. Nat. Biotechnol. 21, 1356 1360.
3. Centonze, V.E. & White, J.G. (1998) Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys. J. 75, 2015 2024.
4. Chu, S.W., Chen, S.Y., Tsai, T.H., Liu, T.M., Lin, C.Y., Tsai, H.J. & Sun, C.K. (2003) In vivo developmental biology study using noninvasive multi-harmonic generation microscopy. Opt. Express 11, 3093 3099.
5. Debarre, D., Supatto, W., Farge, E., Moulia, B., Schanne-Klein, M.C. & Beaurepaire, E. (2004) Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos. Opt. Lett. 29, 2881 2883.
6. Debarre, D., Supatto, W. & Beaurepaire, E. (2005) Structure sensitivity in third-harmonic generation microscopy. Opt. Lett. 30, 2134 2136.
7. Debarre, D., Supatto, W., Pena, A.M. et al 2006) Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy. Nat. Methods 3, 47 53.
8. Filippidis, G., Kouloumentas, C., Voglis, G., Zacharopoulou, F., Papazoglou, T.G. & Tavernarakis, N 2005) Imaging of Caenorhabditis elegans neurons by second harmonic generation and two-photon excitation fluorescence. J. of Biomed. Opt. 10, 024015.
9. Gualda, E.J., Filippidis, G., Voglis, G., Mari, M., Fotakis, C. & Tavernarakis, N 2008) In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy. J. Microsc. 229, 141 150.
10. Mohler, W., Millard, A.C. & Campagnola, P.J. (2003) Second harmonic generation imaging of endogenous structural proteins. Methods 29, 97 109.
11. Moreaux, L., Sandre, O., Blanchard-Desce, M. & Mertz, J. (2000) Membrane imaging by simultaneous second-harmonic and two-photon microscopy. Optics Lett. 25, 320 322.
12. Muller, M., Squier, J., Wilson, K.R. & Brakenhoff, G.J. (1998) 3D microscopy of transparent objects using third-harmonic generation. J. Microsc. 191, 266 274.
13. Pons, T., Moreaux, L., Mongin, O., Blanchard-Desce, M. & Mertz, J 2003) Mechanisms of membrane potential sensing with second-harmonic generation microscopy. J. Biomed. Optics 8, 428 431.
14. Squier, J. & Muller, M. (2001) High resolution nonlinear microscopy: a review of sources and methods for achieving optimal imaging. Rev. Sci. Instrum. 72, 2855 2867.
15. Sun, C.K., Chu, S.W., Chen, S.Y., Tsai, T.H., Liu, T.M., Lin, C.Y. & Tsai, H.J. (2004) Higher harmonic generation microscopy for developmental biology. J. Struct. Biol. 147, 19 30.
16. Syntichaki, P., Troulinaki, K. & Tavernarakis, N. (2007) eIF4E function in somatic cells modulates ageing in Caenorhabditis elegans. Nature 445, 922 926.
17. Troulinaki, K. & Tavernarakis, N. (2005) Neurodegenerative conditions associated with ageing: a molecular interplay? Mech. Ageing Dev. 126, 23 33.
18. Zipfel, W.R., Williams, R.M. & Webb, W.W. (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nature Biotec. 21, 1369 1377.