Characterization of the pH-dependent dissociation of a multimeric metalloprotein Streptomyces rubiginosus xylose isomerase by ESI FT-ICR mass spectrometry

Journal of Mass Spectrometry

Volumn 43, Issue 10, 2008, Pages 1376-1380

  Jänis, Janne ^a   Pasanen, Salla ^a   Rouvinen, Juha ^a   Vainiotalo, Pirjo ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Electrospray ionization; FT ICR mass spectrometry; Metalloprotein; Noncovalent interactions; pH dependent dissociation; Xylose isomerase

Indexed keywords

DISSOCIATION; ENZYMES; FOURIER TRANSFORMS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; MAGNESIUM PRINTING PLATES; MASS SPECTROMETERS; MASS SPECTROMETRY; MONOMERS; OLIGOMERS; POSITIVE IONS; SPECTROMETERS; SPECTROMETRY; SPECTRUM ANALYSIS; SUGAR (SUCROSE); SUGARS; XYLOSE;

FT-ICR MASS SPECTROMETRY; INTERCONVERSION; ION CYCLOTRONS; ISOMERASES; METAL CATIONS; METALLOPROTEIN; MULTIMERIC; NONCOVALENT INTERACTIONS; OCTAMER; PH RANGES; TETRAMER; TETRAMERIC COMPLEXES; XYLOSE ISOMERASE;

ELECTROSPRAY IONIZATION;

KETOSE; MAGNESIUM ION; XYLOSE ISOMERASE;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ELECTROSPRAY; ENZYME STRUCTURE; ION CYCLOTRON RESONANCE MASS SPECTROMETRY; NONHUMAN; PH; PRIORITY JOURNAL; STREPTOMYCES;

ALDOSE-KETOSE ISOMERASES; CATIONS; CYCLOTRONS; FOURIER ANALYSIS; HYDROGEN-ION CONCENTRATION; METALLOPROTEINS; PROTEIN CONFORMATION; PROTEIN DENATURATION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STREPTOMYCES;

STREPTOMYCES RUBIGINOSUS;

EID: 55249116040     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1413     Document Type: Article

References (18)

1. Sharon M, Robinson CV. The role of mass spectrometry in structure elucidation of dynamic protein complexes. Annual Review of Biochemistry 2007; 76: 167.
2. Hernández H, Robinson CV. Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry. Nature Protocols 2007; 2: 715.
3. Heck AJR, van den Heuvel RHH. Investigation of intact protein complexes by mass spectrometry. Mass Spectrometry Reviews 2004; 23: 368.
4. Asbótha B, Náray-Szabób G. Mechanism of action of D-xylose isomerase. Current Protein and Peptide Science 2000; 1: 237.
5. Hartley BS, Hanlon N, Jackson RJ, Rangarajan M. Glucose isomerase: insights into protein engineering for increased thermostability. Biochimica et Biophysica Acta 2000; 29: 294.
6. Jokela J, Pastinen O, Leisola M. Isomerization of pentose and hexose sugars by an enzyme reactor packed with cross-linked xylose isomerase crystals. Enzyme and Microbial Technology 2002; 31: 67.
7. Carrell HL, Rubin BH, Hurley TJ, Glusker JP. X-ray crystal structure of D-xylose isomerase at 4-Å resolution. The Journal of Biological Chemistry 1984; 259: 3230.
8. Carrell HL, Glusker JP, Burger V, Manfre F, Tritsch D, Biellmann JF. X-ray analysis of D-xylose isomerase at 1.9 Å, native enzyme in complex with substrate andwith a mechanism-designed inactivator. Proceedings of the National Academy of Sciences of the United States of America 1989; 86: 4440.
9. Cha J, Cho Y, Whitaker RD, Carrell HL, Glusker JP, Karplus PA, Batt CA. Perturbing the metal site in D-Xylose isomerase: effect of mutations of His220 on enzyme stability. The Journal of Biological Chemistry 1994; 4: 2687.
10. Marshall AG, Hendrickson CL, Jackson GS. Fourier transform ion cyclotron resonance mass spectrometry - a primer. Mass Spectrometry Reviews 1998; 17: 1.
11. Hampton research users guide for Streptomyces rubiginosus glucose isomerase 2007; (available at http://www.hamptonresearch.com/products/) [accessed 2007].
12. Jänis J, Turunen O, Leisola M, Derrick PJ, Rouvinen J, Vainiotalo P. Characterization of mutant xylanases using fourier transform ion cyclotron resonance mass spectrometry: stabilizing contributions of disulfide bridges and n-terminal extensions. Biochemistry 2004; 43: 9556.
13. Wang W, Kitova EN, Klassen JS. Influence of solution and gas phase processes on protein-carbohydrate binding affinities determined by nanoelectrospray Fourier transform ion cyclotron resonancemass spectrometry. Analytical Chemistry 2003; 75: 4945.
14. Hunt SM, Sheil MM, Belov M, Derrick PJ. Probing the effects of cone potential in the electrospray ion source: consequences for the determination of molecular weight distributions of synthetic polymers. Analytical Chemistry 1998; 70: 1812.
15. Sze T-PE, Dominic Chan T-W. Time-of-flight effects in matrix-assisted laser desorption/ionization Fourier transform mass spectrometry. Rapid Communications in Mass Spectrometry 1999; 13: 398.
16. O'Connor PN, Duursma MC, van Rooij GJ, Heeren RMA, Boon JJ. Correction of time-of-flight shifted polymeric molecular weight distributions in matrix-assisted laser desorption/ionization Fourier transform mass spectrometry. Analytical Chemistry 1997; 69: 2751.
17. Pastinen O. Xylose isomerase from Streptomyces rubiginosus: stability, novel reactions and applications. Ph.D. Thesis, Helsinki University of Technology, Espoo, 2000.
18. Collaborative Computational Project, Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallographica 1994; D50: 760.