A comparison of serum and EDTA plasma in the measurement of glutamic acid decarboxylase autoantibodies (GADA) and autoantibodies to islet antigen-2 (IA-2A) using the RSR radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) kits

Clinical Laboratory

Volumn 54, Issue 7-8, 2008, Pages 227-235

  Rahmati, Kobra ^a   Lernmark, Åke ^b   Becker, Charlotte ^b   Foltyn Zadura, Anna ^b   Larsson, Karin ^c   Ivarsson, Sten Anders ^b   Törn, Carina ^a  

^a Department of Endocrinology   (Sweden)

^b LUND UNIVERSITY HOSPITAL   (Sweden)

^c CENTRAL HOSPITAL   (Sweden)

Author keywords

AUC; ELISA; RBA; Sensitivity; Specificity

Indexed keywords

AUTOANTIBODY; CALCIUM CHLORIDE; EDETIC ACID; GLUTAMATE DECARBOXYLASE ANTIBODY; ISLET ANTIGEN 2 ANTIBODY; UNCLASSIFIED DRUG;

ADOLESCENT; ANALYTIC METHOD; ANTIBODY BLOOD LEVEL; ANTIBODY DETECTION; AREA UNDER THE CURVE; ARTICLE; CHILD; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; INSULIN DEPENDENT DIABETES MELLITUS; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; PLASMA; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; SERUM;

ADOLESCENT; AREA UNDER CURVE; AUTOANTIBODIES; AUTOANTIGENS; CHILD; CHILD, PRESCHOOL; DIABETES MELLITUS, TYPE 1; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLUTAMATE DECARBOXYLASE; HUMANS; MALE; PLASMA; RADIOIMMUNOASSAY; REAGENT KITS, DIAGNOSTIC; RECEPTOR-LIKE PROTEIN TYROSINE PHOSPHATASES, CLASS 8; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SERUM;

EID: 55049133519     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Bottazzo GF, Florin-Christensen A, Doniach D. Islet-cell anti-bodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet 1974; 2: 1279-1283
2. Palmer JP, Asplin CM, Clemons P, et al. Insulin antibodies in insulin-dependent diabetics before insulin treatment. Science 1983; 222: 1337-1339
3. Grubin CE, Daniels T, Toivola B, et al. A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM. Diabetologia 1994; 37: 344-350
4. Falorni A, Ortqvist F, Persson B, Lernmark A. Radioimmunoassays for glutamic acid decarboxylase (GAD65) and GAD65 autoantibodies using 35S or 3H recombinant human ligands. J Immunol Methods 1995; 186: 89-99
5. Gianani R, Rabin DU, Verge CF, et al. ICA512 autoantibody radioassay. Diabetes 1995; 44: 1340-1344
6. Karlsen AE, Hagopian WA, Grubin CE, et al. Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10. Proc Natl Acad Sci USA 1991; 88: 8337-8341
7. Boitard C, Bonifacio E, Bottazzo GF, Gleichmann H, Molenaar J. Immunology and Diabetes Workshop: report on the Third International (Stage 3) Workshop on the Standardisation of Cytoplasmic Islet Cell Antibodies. Held in New York, New York, October 1987. Diabetologia 1988; 31: 451-452
8. Bonifacio E, Boitard C, Gleichmann H, Shattock MA, Molenaar JL, Bottazzo GF. Assessment of precision, concordance, specificity, and sensitivity of islet cell antibody measurement in 41 assays. Diabetologia 1990; 33: 731-736
9. Palmer JP, Wilkin TJ, Kurtz AB, Bonifacio E. The Third International Workshop on the Standardisation of Insulin Autoantibody Measurement Diabetologia 1990; 33: 60-61
10. Schmidli RS, Colman PG, Bonifacio E, Bottazzo GF, Harrison LC. High level of concordance between assays for glutamic acid decarboxylase antibodies. The First International Glutamic Acid Decarboxylase Antibody Workshop. Diabetes 1994; 43: 1005-1009
11. Schmidli RS, Colman PG, Bonifacio E. Disease sensitivity and specificity of 52 assays for glutamic acid decarboxylase antibodies. The Second International GADAB Workshop. Diabetes 1995; 44: 636-640
12. Verge CF, Stenger D, Bonifacio E, et al. Combined use of autoantibodies (IA-2 autoantibody, GAD autoantibody, insulin autoantibody, cytoplasmic islet cell antibodies) in type I diabetes: Combinatorial Islet Autoantibody Workshop. Diabetes 1998; 47: 1857-1866
13. Bingley PJ, Bonifacio E, Mueller PW. Diabetes Antibody Standardization Program: first assay proficiency evaluation. Diabetes 2003; 52: 1128-1136
14. Torn C, Mueller PW, Schlosser M, Bonifacio E, Bingley PJ. Diabetes Antibody Standardization Program: evaluation of assays for autoantibodies to glutamic acid decarboxylase and islet antigen-2. Diabetologia 2008; 51: 846-852
15. Nilson E, Ekholm B, Rees Smith B, Tom C, Hillman M. Calcium addition to EDTA plasma eliminates falsely positive results in the RSR GADAb ELISA. Clin Chim Acta 2008; 388: 130-134
16. Lindberg B, Ahlfors K, Carlsson A, et al. Previous exposure to measles, mumps, and rubella-but not vaccination during adolescence-correlates to the prevalence of pancreatic and thyroid autoantibodies. Pediatrics 1999; 104: e12
17. Mire-Sluis AR, Gaines Das R, Lernmark A. The World Health Organization International Collaborative Study for islet cell antibodies. Diabetologia 2000; 43: 1282-1292
18. Hampe CS, Hammerle LP, Bekris L, et al. Recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different GAD antibody-positive phenotypes. J Clin Endocrinol Metab 2000; 85: 4671-4679
19. Hampe CS, Kockum I, Landin-Olsson M, et al. GAD65 antibody epitope patterns of type 1. 5 diabetic patients are consistent with slow-onset autoimmune diabetes. Diabetes Care 2002; 25: 1481-1482