Calcium addition to EDTA plasma eliminates falsely positive results in the RSR GADAb ELISA

Clinica Chimica Acta

Volumn 388, Issue 1-2, 2008, Pages 130-134

  Nilson, Emma ^a   Ekholm, Birgitte ^b   Rees Smith, Bernard ^b   Törn, Carina ^c   Hillman, Magnus ^a  

^a LUND UNIVERSITY   (Sweden)

^b RSR LTD   (United Kingdom)

^c LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Autoantibodies; Diabetes type 1; ELISA

Indexed keywords

ANTIBODY; CALCIUM; EDETIC ACID; GAD ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; DIABETES MELLITUS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; MAJOR CLINICAL STUDY; PLASMA; PREVALENCE; PRIORITY JOURNAL; SERUM;

AUTOANTIBODIES; CALCIUM; EDETIC ACID; ENZYME-LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE REACTIONS; GLUTAMATE DECARBOXYLASE; HUMANS; PLASMA;

EID: 37249029951     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2007.10.021     Document Type: Article

References (19)

1. Notkins A.L., and Lernmark A. Autoimmune type 1 diabetes: resolved and unresolved issues. J Clin Invest 108 (2001) 1247-1252
2. Dean B.M., Pujol-Borrell R., and Botazzo G.F. Determination of islet cell antibodies by immunofluorescence. Lancet 2 (1982) 1343-1344
3. Bottazzo G., and Gleichmann H. Immunology and Diabetes Workshops: report of the first international workshop on the standardisation of cytoplasmic islet cell antibodies. Diabetologia 29 (1986) 125-126
4. Atkinson M.A., Maclaren N.K., Scharp D.W., Lacy P.E., and Riley W.J. 64,000 Mr autoantibodies as predictors of insulin-dependent diabetes. Lancet 335 (1990) 1357-1360
5. Baekkeskov S., Aanstoot H.J., Christgau S., Reetz A., Solimena M., Cascalho M., et al. Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature 347 (1990) 151-156
6. Karlsen A.E., Hagopian W.A., Petersen J.S., Boel E., Dyrberg T., Grubin C.E., et al. Recombinant glutamic acid decarboxylase (representing the single isoform expressed in human islets) detects IDDM-associated 64,000-M(r) autoantibodies. Diabetes 41 (1992) 1355-1359
7. Passini N., Larigan J.D., Genovese S., Appella E., Sinigaglia F., and Rogge L. The 37/40-kilodalton autoantigen in insulin-dependent diabetes mellitus is the putative tyrosine phosphatase IA-2. Proceedings of the National Academy of Sciences of the United States of America 92 (1995) 9412-9416
8. Christie M., Landin-Olsson M., Sundkvist G., Dahlquist G., Lernmark A., and Baekkeskov S. Antibodies to a Mr-64,000 islet cell protein in Swedish children with newly diagnosed type 1 (insulin-dependent) diabetes. Diabetologia 31 (1988) 597-602
9. Rabin D.U., Pleasic S.M., Shapiro J.A., Yoo-Warren H., Oles J., Hicks J.M., et al. Islet cell antigen 512 is a diabetes-specific islet autoantigen related to protein tyrosine phosphatases. J Immunol 152 (1994) 3183-3188
10. Masuda M., Powell M., Chen S., Beer C., Fichna P., Rees Smith B., et al. Autoantibodies to IA-2 in insulin-dependent diabetes mellitus. Measurements with a new immunoprecipitation assay. Clin Chim Acta 291 (2000) 53-66
11. Bingley P.J., Bonifacio E., and Mueller P.W. Diabetes Antibody Standardization Program: first assay proficiency evaluation. Diabetes 52 (2003) 1128-1136
12. Falorni A., Ortqvist E., Persson B., and Lernmark A. Radioimmunoassays for glutamic acid decarboxylase (GAD65) and GAD65 autoantibodies using 35S or 3H recombinant human ligands. J Immunol Methods 186 (1995) 89-99
13. Grubin C.E., Daniels T., Toivola B., Landin-Olsson M., Hagopian W.A., Li L., et al. A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM. Diabetologia 37 (1994) 344-350
14. Powell M., Prentice L., Asawa T., Kato R., Sawicka J., Tanaka H., et al. Glutamic acid decarboxylase autoantibody assay using 125I-labelled recombinant GAD65 produced in yeast. Clin Chim Acta 256 (1996) 175-188
15. Brooking H., Ananieva-Jordanova R., Arnold C., Amoroso M., Powell M., Betterle C., et al. A sensitive non-isotopic assay for GAD65 autoantibodies. Clinica Chimica Acta 331 (2003) 55-59
16. Chen S., Willis J., Maclean C., Ananieva-Jordanova R., Amoroso M.A., Brooking H., et al. Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65. Clin Chim Acta 357 (2005) 74-83
17. Chakravarti I.M., Laha R.G., and Roy J. Handbook of methods of applied statistics (1967), Wiley, New York
18. Krouwer J.S., and Monti K.L. A simple, graphical method to evaluate laboratory assays. Eur J Clin Chem Clin Biochem 33 (1995) 525-527
19. Ludbrook J. Statistical techniques for comparing measurers and methods of measurement: a critical review. Clin Exp Pharmacol Physiol 29 (2002) 527-536