The use of a nested PCR-RFLP technique, based on the parasite's 18S ribosomal RNA, to characterise Cryptosporidium isolates from HIV/AIDS patients

Annals of Tropical Medicine and Parasitology

Volumn 102, Issue 7, 2008, Pages 597-601

  Zavvar, M ^a   Sadraei, J ^a   Emadi, H ^b   Pirestani, M ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 18S;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; DISEASE ASSOCIATION; DISEASE PREDISPOSITION; FECES ANALYSIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; POLYMERASE CHAIN REACTION; PROTOZOAL GENETICS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

AIDS-RELATED OPPORTUNISTIC INFECTIONS; ANIMALS; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; ELECTROPHORESIS; FECES; HIV INFECTIONS; HUMANS; IMMUNOCOMPROMISED HOST; IRAN; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 18S;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM;

EID: 55049087521     PISSN: 00034983     EISSN: None     Source Type: Journal    
DOI: 10.1179/136485908X311876     Document Type: Article

References (25)

1. Akiyoshi, D. E., Feng, X., Buckholt, M. A., Widmer, G. & Tzipori, S. (2002). Genetic analysis of a Cryptosporidium parvum human genotype I isolate passaged through different host species. Infection and Immunity, 70, 5670-5675.
2. Akiyoshi, D. E., Dilo, J., Pearson, C., Chapman, S., Tumwine, J. & Tzipori, S. (2003). Characterization of Cryptosporidium meleagridis of human origin passaged through different host species. Infection and Immunity, 71, 1828-1832.
3. Chalmers, R.,M., Christobel, F., Cacciò, S., Gasser, R.,B., Abs El-Ostad, Y. G., Heijnen, L., Xiao, L., Elwin, K., Hadfield, S., Sinclair, M. & Stevens, M. (2005). Direct comparison of selected methods for genetic categorization of Cryptosporidium parvum and Cryptosporidium hominis species. International Journal for Parasitology, 35, 397-410.
4. Coupe, S., Sarfati, C., Hamane, S. & Derouin, F. (2005). Detection of Cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. Journal of Clinical Microbiology, 43, 1017-1023.
5. Fayer, R., Morgan, U. M. & Upton, S. J. (2000). Epidemiology of Cryptosporidium: transmission, detection and identification. Journal of Parasitology, 30, 1305-1322.
6. Gasser, R. B., Abs El-Osta, Y. G., Prepens, S. & Chalmers, R. M. (2004). An improved 'cold SSCP' method for the genotypic and subgenotypic characterization of Cryptosporidium. Molecular and Cellular Probes, 18, 329-332.
7. Gatei, W., Greensill, J., Ashford, R. W., Cuevas, L. E., Parry, C. M., Cunliffe, N. A., Beeching, N. J. & Hart, C. A. (2003). Molecular analysis of the 18S rRNA gene of Cryptosporidium parasites from patients with or without human immunodeficiency virus infections living in Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. Journal of Clinical Microbiology, 41, 1458-1462.
8. Hajdusek, O., Ditrich, O. & Slapeta, J. (2004). Molecular identification of Cryptosporidium spp. in animal and human hosts from the Czech Republic. Veterinary Parasitology, 122, 183-192.
9. Leoni, F., Gallimore, C. I., Green, J. & McLauchlin, J. (2003). A rapid method for identifying diversity within PCR amplicons using a heteroduplex mobility assay and synthetic polynucleotides: application to characterisation of dsRNA elements associated with Cryptosporidium. Journal of Microbiological Methods, 54, 95-103.
10. Limor, J. R., Lal, A. A. & Xiao, L. (2002). Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. Journal of Clinical Microbiology, 40, 2335-2338.
11. McLauchlin, J., Pedraza-Diaz, S., Amar-Hoetzeneder, C. & Nichols, G. L. (1999). Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis. Journal of Clinical Microbiology, 37, 3153-3158.
12. McLauchlin, J., Amar, C., Pedraza-Diaz, S. & Nichols, G. L. (2000). Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1705 fecal samples from humans and 105 fecal samples from livestock animals. Journal of Clinical Microbiology, 38, 3984-3990.
13. Morgan, U. M., Fall, A., Ward, L. A., Hijjawi, N., Sulaiman, I. M., Fayer, R., Thompson, R. C. A., Olson, M., Lal, A. A. & Xiao, L. (2002). Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. Journal of Eukaryotic Microbiology, 49, 433-440.
14. Park, J. H., Guk, S. M., Han, E. T., Shin, E. H., Kim, J. L. & Chai, J. Y. (2006). Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea. Korean Journal of Parasitology, 44, 27-33.
15. Schindler, A. R., Abs El-Osta, Y. G., Stevens, M., Sinclair, M. I. & Gasser, R. B. (2005). Capillary electrophoretic analysis of fragment length polymorphism in ribosomal markers of Cryptosporidium from humans. Molecular and Cellular Probes, 19, 394-399.
16. Spano, F. & Crisanti, A. (2000). The initiation translation factor eIF-4A of Cryptosporidium parvum is encoded by two distinct mRNA forms and shows DNA sequence polymorphism distinguishing genotype 1 and 2 isolates. Journal of Parasitology, 86, 777-782.
17. Straub, T. M., Daly, D. S., Wunshel, S., Rochelle, P. A., DeLeon, R. & Chandler, D. P. (2002). Genotyping Cryptosporidium parvum with an hsp70 single-nucleotide polymorphism microarray. Applied and Environmental Microbiology, 68, 1817-1826.
18. Sulaiman, I. M., Morgan, U. M., Thompson, R. C. A. Lal, A. A. & Xiao, L. (2000). Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene. Applied and Environmental Microbiology, 66, 2385-2391.
19. Widmer, G., Tzipori, S., Fichtenbaum, C. J. & Griffiths, J. K. (1998). Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS. Journal of Infectious Diseases, 178, 834-840.
20. Wu, Z., Nagano, I., Boonmars, T. & Takahashi, Y. (2004). Further evidence that genotype I and genotype II of Cryptosporidium parvum are distinct. Tropical Medicine and Health, 32, 5-14.
21. Xiao, L., Escalante, L., Yang, C., Sulaiman, I. M., Escalante, A. A., Montali, R. J., Fayer, R. & Lal, A. A. (1999a). Phylogenetic analysis of Cryptosporidium parasites based on the small subunit rRNA gene locus. Applied and Environmental Microbiology, 65, 1578-1583.
22. Xiao, L., Morgan, U. M., Limor, J., Escalante, A., Arrowood, M., Shulaw, W., Thompson, R. C. A., Fayer, R. & Lal, A. A. (1999b). Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Applied and Environmental Microbiology, 65, 3386-3391.
23. Xiao, L., Bern, C., Limor, J., Sulaiman, I. M., Roberts, J., Checkley, W., Cabrera, L., Gilman, R. H. & Lal, A. A. (2001). Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. Journal of Infectious Diseases, 183, 492-497.
24. Xiao, L., Sulaiman, I. M., Morgan, U. M., Zhou, L., Atwill, E. R., Tischler, M. L., Zhang, X., Fayer, R. & Lal, A. A. (2002). Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Journal of Parasitology, 32, 1773-1785.
25. Xiao, L., Fayer, R., Morgan, U. M. & Upton, S. J. (2004). Cryptosporidium: taxonomy, recent advances and implications for public health. Clinical Microbiology Reviews, 17, 72-97.