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Volumn 79, Issue 9, 2008, Pages

Multiplexed single-molecule measurements with magnetic tweezers

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

EID: 54749117023     PISSN: 00346748     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.2981687     Document Type: Article
Times cited : (140)

References (17)
  • 10
    • 54749109962 scopus 로고    scopus 로고
    • Hydrodynamic coupling is properly described through a diffusivity or mobility tensor that indicates how motion of bead 1 in direction i affects the motion of bead 2 in direction j. Here, we quote the largest coupling parameter from that tensor (that for bead motion parallel to the bead-bead separation direction). See Ref. for details.
    • Hydrodynamic coupling is properly described through a diffusivity or mobility tensor that indicates how motion of bead 1 in direction i affects the motion of bead 2 in direction j. Here, we quote the largest coupling parameter from that tensor (that for bead motion parallel to the bead-bead separation direction). See Ref. for details.
  • 11
    • 0000298119 scopus 로고    scopus 로고
    • 0031-9007 10.1103/PhysRevLett.82.2211.
    • J. C. Meiners and S. R. Quake, Phys. Rev. Lett. 0031-9007 10.1103/PhysRevLett.82.2211 82, 2211 (1999).
    • (1999) Phys. Rev. Lett. , vol.82 , pp. 2211
    • Meiners, J.C.1    Quake, S.R.2
  • 14
    • 54749139292 scopus 로고    scopus 로고
    • We judged successful fixing by measuring the power spectra of the traces in all three dimensions. For nonspecifically stuck beads prior to heating, the power spectra exhibited a decrease in amplitude with frequency, indicating that the beads were undergoing constrained Brownian motion. After heating, the power spectra amplitudes were constant with frequency, indicating that the beads were well fixed to the surface, leaving only the white noise intrinsic to the measurement.
    • We judged successful fixing by measuring the power spectra of the traces in all three dimensions. For nonspecifically stuck beads prior to heating, the power spectra exhibited a decrease in amplitude with frequency, indicating that the beads were undergoing constrained Brownian motion. After heating, the power spectra amplitudes were constant with frequency, indicating that the beads were well fixed to the surface, leaving only the white noise intrinsic to the measurement.
  • 15
    • 54749119745 scopus 로고    scopus 로고
    • The effects of varying certain parameters of the microscope and tracking routine were tested. In the range of 50×-150× magnification, tracking accuracy in z was negligibly affected by the variation of the following parameters: window size (provided the first diffraction ring is entirely included), background intensity above a particular illumination strength, focal position (provided that the focal plane is within the range of calibration), and illumination numerical aperture.
    • The effects of varying certain parameters of the microscope and tracking routine were tested. In the range of 50×-150× magnification, tracking accuracy in z was negligibly affected by the variation of the following parameters: window size (provided the first diffraction ring is entirely included), background intensity above a particular illumination strength, focal position (provided that the focal plane is within the range of calibration), and illumination numerical aperture.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.