Isolation and characterization of endothelial progenitor cells from human blood

Current Protocols in Stem Cell Biology

Volumn , Issue SUPPL. 6, 2008, Pages

  Mead, Laura E ^a,b   Prater, Daniel ^a,b   Yoder, Mervin C ^a,b   Ingram, David A ^a,b  

^a INDIANA UNIVERSITY   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adult progenitor cells; Endothelial cell transplantation; Endothelial colony forming cells (ECFC); Endothelial progenitor cell (EPC)

Indexed keywords

BIOLOGICAL MARKER; ACETYL LOW DENSITY LIPOPROTEIN; ACETYL-LDL; CD31 ANTIGEN; COLLAGEN; LAMININ; LOW DENSITY LIPOPROTEIN; MATRIGEL; MEMBRANE ANTIGEN; PROTEOGLYCAN;

ANGIOGENESIS; CARDIOVASCULAR RISK; CELL FUNCTION; CELL ISOLATION; CELL PROLIFERATION; CELL TYPE; COLONY FORMATION; COLONY FORMING CELL; EMBRYO DEVELOPMENT; ENDOTHELIUM CELL; HUMAN; IN VIVO STUDY; MACROPHAGE; METHODOLOGY; MOLECULAR CLONING; MONOCYTE; MOUSE; NONHUMAN; PERIPHERAL BLOOD STEM CELL; PHENOTYPE; PRIORITY JOURNAL; REVIEW; RISK ASSESSMENT; SCID MOUSE; STEM CELL TRANSPLANTATION; UMBILICAL CORD BLOOD; ADIPOSE TISSUE; ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BLOOD CELL; CELL CLONE; CELL SEPARATION; CELL TRANSPLANTATION; CLONOGENIC ASSAY; CRYOPRESERVATION; CYTOLOGY; DRUG COMBINATION; IMMUNOHISTOCHEMISTRY; METABOLISM; STEM CELL; STROMA CELL; UMBILICAL CORD;

MUS;

ADIPOSE TISSUE; ANIMALS; ANTIGENS, CD31; ANTIGENS, SURFACE; BLOOD CELLS; CELL PROLIFERATION; CELL SEPARATION; CELL TRANSPLANTATION; CLONE CELLS; COLLAGEN; COLONY-FORMING UNITS ASSAY; CRYOPRESERVATION; DRUG COMBINATIONS; ENDOTHELIAL CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; LAMININ; LIPOPROTEINS, LDL; MICE; ORGAN SPECIFICITY; PHENOTYPE; PROTEOGLYCANS; STEM CELLS; STROMAL CELLS; UMBILICAL CORD;

EID: 54449093958     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc02c01s6     Document Type: Review

References (28)

1. Asahara, T., Murohara, T., Sullivan A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schattman G., and, Isner J.M., 1997. Isolation of putative progenitor endothelial cells for angiogenesis. Science 275: 964 - 967.
2. Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Silver, M., Kearne, M., Magner, M., and, Isner, J.M., 1999. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ. Res. 85: 221 - 228.
3. Bancroft, J.D., and, Gamble, M., 2002. Theory and Practice of Histological Techniques. 5th Ed.. Churchill Livingstone, New York.
4. Baumgarth, N., and, Roederer, M., 2000. A practical approach to multicolor flow cytometry for immunophenotyping. J. Immunol. Methods 243: 77 - 97.
5. Bompais, H., Chagraoui, J., Canron, X., Crisen, M., Liu, X.H., Anjo, A., Tolla-LePort, C., Leboef, M., Charbord, P., Bikfalvi, A., and, Uzan, G., 2004. Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells. Blood 103: 2577 - 2584.
6. Dimmeler, S., Aicher, A., Vasa, M., Mildner-Rihm, C., Adler, K., Tiemann, M., Rutten, H., Fichtlscherer, S., Martin, H., and, Zeiher, M., 2001. HMG-CoA reductase inhibitors (statins) increase endothelial progenitor cells via the PI 3-kinase/Akt pathway. J. Clin. Invest. 108: 391 - 397.
7. Donovan, J., and, Brown, P., 2006. Euthanasia. Curr. Protoc. Immunol. 73: 1.8.1 - 1.8.4.
8. Enis, D.R., Shepherd, B.R., Wang, R., Qasim, A., Shanahan, C.M., Weissberg, P.L., Kashgarian, M., Pober, J.S., and, Schechner, J.S., 2005. Induction, differentiation, and remodeling of blood vessels after transplantation of Bcl-2-transduced endothelial cells. Proc. Natl. Acad. Sci. U.S.A 102: 425 - 430.
9. Gulati, R., Jevremovic, D., Peterson, T.E., Chaterjee, S., Shah, V., Vile, R.G., and, Simon, R.D., 2003. Diverse origin and function of cells with endothelial phenotype obtained from adult human blood. Circ. Res. 93: 1023 - 1025.
10. Hassan, N.F., Campbell, D.E., and, Douglas, S.D., 1986. Purification of human monocytes on gelatin-coated surfaces. J. Immunol. Methods 95: 273 - 276.
11. Hill, J.M., Zalos, G., Halcox, J.P., Schenke, W.H., Waclawiw, M.A., Quyyumi, A.A., and, Finkel, T., 2003. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N. Engl. J. Med. 348: 593 - 600.
12. Hur, J., Yoon, C.H., Kim, H.S., Choi, J.H., Kang, H.J., Hwang, K.K., Oh, B.H., Lee, M.M., and, Park, Y.B., 2004. Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler. Thromb. Vasc. Biol. 24: 288 - 293.
13. Ingram, D.A., Mead, L.E., Tanaka, H., Meade, V., Fenoglio, A., Mortell, K., Pollok, K., Ferkowicz, M.J., Gilley, D., and, Yoder, M.C., 2004. Identification of a novel hierarchy of endothelial progenitor cells utilizing human peripheral and umbilical cord blood. Blood 104: 2752 - 2760.
14. Ito, H., Rovira, I.I., Bloom, M.L., Takeda, K., Ferrans, V.J., Quyyumi, A.A., and, Finkel, T., 1999. Endothelial progenitor cells as putative targets for angiostatin. Cancer Res. 59: 5875 - 5877.
15. Kalka, C., Masuda, H., Takahashi, T., Kalka-Moll, W.M., Silver, M., Kearney, M., Li, T., Isner, J.M., and, Asahara, T., 2000. Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc. Natl. Acad. Sci. U.S.A. 97: 3422 - 3427.
16. Lin, Y., Weisdorf, D.J., Solovey, A., and, Hebbel, R.P., 2000. Origins of circulating endothelial cells and endothelial outgrowth from blood. J. Clin. Invest. 105: 71 - 77.
17. Melero-Martin, J.M., Khan, Z.A., Picard, A., Wu, X., Paruchuri, S., and, Bischoff, J., 2007. In vivo vasculogenic potential of human blood-derived endothelial progenitor cells. Blood 109: 4761 - 4768. (Pubitemid 46827769)
18. Perfetto, S.P., Ambrozak, D., Nguyen, R., Chattopadhyay, P., and, Roederer, M., 2006. Quality assurance for polychromatic flow cytometry. Nat. Protoc. 1: 1522 - 1530.
19. Prater, D.N., Case, J., Ingram, D.A., and, Yoder, M.C., 2007. Working hypothesis to redefine endothelial progenitor cells. Leukemia 21: 1141 - 1149.
20. Rafii, S., and, Lyden, D., 2003. Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat. Med. 9: 702 - 712.
21. Rehman, J., Li, J., Orschell, C.M., and, March, K.L., 2003. Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors." Circulation 107: 1164 - 1169.
22. Schechner, J.S., Nath, A.K., Zheng, L., Kluger, M.S., Hughes, C.C., Sierra-Honigmann, M.R., Lorber, M.I., Tellides, G., Kashgarian, M., Bothwell, A.L., and, Pober, J.S., 2000. In vivo formation of complex microvessels lined by human endothelial cells in an mmunodeficient mouse. Proc. Natl. Acad. Sci. U.S.A. 97: 9191 - 9196.
23. Schmeisser, A., Graffy, C., Daniel, W.G., and, Strasser, R.H., 2003. Phenotypic overlap between monocytes and vascular endothelial cells. Adv. Exp. Med. Biol. 522: 59 - 74.
24. Shapiro, H.M., 2003. Practical Flow Cytometry. 4th Edition. Wiley-Liss, Wilmington, Del.
25. Shepherd, B.R., Enis, D.R., Wang, F., Suarez, Y., Pober, J.S., and, Schechner, J.S., 2006. Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothelial cells. Faseb J. 20: 1739 - 1741.
26. Werner, N., Kosiol, S., Schiegl, T., Ahlers, P., Walenta, K., Link, A., Bhm, M., and, Nickenig, G., 2005. Circulating endothelial progenitor cells and cardiovascular outcomes. N. Engl. J. Med. 353: 999 - 1007. (Pubitemid 41262334)
27. Yoder, M.C., Mead, L.E., Prater, D., Krier, T.R., Mroueh, K.N., Li, F., Krasich, R., Temm, C.J., Prchal, J.T., and, Ingram, D.A., 2007. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals. Blood 109: 1801 - 1809.
28. Ziegelhoeffer, T., Fernandez, B., Kostin, S., Heil, M., Voswinckel, R., Helisch, A., Kostin S, Heil M, Voswinckel R., and, Helisch, A., 2004. Bone marrow-derived cells do not incorporate into the adult growing vasculature. Circ. Res. 94: 230 - 238.