Application of molecular fingerprinting for qualitative assessment of small-intestinal bacterial diversity in dogs

Journal of Clinical Microbiology

Volumn 42, Issue 10, 2004, Pages 4702-4708

  Suchodolski, Jan S ^a   Ruaux, Craig G ^a   Steiner, Jörg M ^a   Fetz, Kathrin ^a   Williams, David A ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA 16S;

AMPLICON; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA FINGERPRINTING; DOG; DUODENUM FLUID; GASTROINTESTINAL ENDOSCOPY; MICROBIAL DIVERSITY; MICROFLORA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITATIVE DIAGNOSIS; REPRODUCIBILITY; SMALL INTESTINE;

ANIMALS; BACTERIA; DNA FINGERPRINTING; DNA, BACTERIAL; DOGS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; INTESTINE, SMALL; MALE; VARIATION (GENETICS);

ANIMALIA; BACTERIA (MICROORGANISMS); CANIS FAMILIARIS;

EID: 5444258505     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.10.4702-4708.2004     Document Type: Article

References (28)

1. Amann, R. I., W. Ludwig, and K. H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.
2. Batt, R. M., J. R. Needham, and M. W. Carter. 1983. Bacterial overgrowth associated with a naturally occurring enteropathy in the German shepherd dog. Res. Vet. Sci. 35:42-46.
3. Brooks, S. P., M. McAllister, M. Sandoz, and M. L. Kalmokoff. 2003. Culture-independent phylogenetic analysis of the faecal flora of the rat. Can. J. Microbiol. 49:589-601.
4. Collins, K., and L. O'Mahony. 2002. The "unculturables," p. 25-33. In A. L. Hart, A. J. Stagg, H. Graffner, H. Glise, P. Falk, and M. A. Kamm (ed.), Gut ecology. Martin Dunitz Ltd., London, United Kingdom.
5. Delles, E. K., M. D. Willard, R. B. Simpson, T. W. Fossum, M. Slater, D. Kolp, G. E. Lees, R. Helman, and G. Reinhart. 1994. Comparison of species and numbers of bacteria in concurrently cultured samples of proximal small intestinal fluid and endoscopically obtained duodenal mucosa in dogs with intestinal bacterial overgrowth. Am. J. Vet. Res. 55:957-964.
6. Fromin, N., J. Hamelin, S. Tarnawski, D. Roesti, K. Jourdain-Miserez, N. Forestier, S. Teyssier-Cuvelle, F. Gillet, M. Aragno, and P. Rossi. 2002. Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns. Environ. Microbiol. 4:634-643.
7. Harmonien, J. A., J. M. Matto, M. L. Rinkinen, M. Wilsson-Rahmberg, and E. Westermarck. 2001. Permanent jejunal fistula: promising method for obtaining small intestinal chyme without disturbing intestinal function. Comp. Med. 51:252-256.
8. Hugenholtz, P., C. Pitulle, K. L. Hershberger, and N. R. Pace. 1998. Novel division-level bacterial diversity in a Yellowstone hot spring. J. Bacteriol. 180:366-376.
9. Johnston, K. L. 1999. Small intestinal bacterial overgrowth. Vet. Clin. North Am. Small Anim. Pract. 29:523-550.
10. Johnston, K. L., A. Lamport, O. Ballevre, and R. M. Batt. 1999. A comparison of endoscopic and surgical collection procedures for the analysis of the bacterial flora in duodenal fluid from cats. Vet. J. 157:85-89.
11. Kaneko, Y., H. Takeshita, K. Mogi, T. Nakajima, T. Yasuda, M. Itoi, H. Kuwano, and K. Kishi. 2003. Molecular, biochemical and immunological analyses of canine pancreatic DNase I. J. Biochem. (Tokyo) 134:711-718.
12. Langendijk, P. S., F. Schut, G. J. Jansen, G. C. Raangs, G. R. Kamphuis, M. H. Wilkinson, and G. W. Welling. 1995. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl. Environ. Microbiol. 61:3069-3075.
13. Leser, T. D., J. Z. Amenuvor, T. K. Jensen, R. H. Lindecrona, M. Boye, and K. Moller. 2002. Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Appl. Environ. Microbiol. 68:673-690.
14. McCracken, V. J., J. M. Simpson, R. I. Mackie, and H. R. Gaskins. 2001. Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota. J. Nutr. 131:1862-1870.
15. Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
16. Muyzer, G., and K. Smalla. 1998. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek 73:127-141.
17. Palka-Santini, M., B. Schwarz-Herzke, M. Hosel, D. Renz, S. Auerochs, H. Brondke, and W. Doerfler. 2003. The gastrointestinal tract as the portal of entry for foreign macromolecules: fate of DNA and proteins. Mol. Genet. Genomics 270:201-215.
18. Papasouliotis, K., A. H. Sparkes, G. Werrett, K. Egan, E. A. Gruffydd-Jones, and T. J. Gruffydd-Jones. 1998. Assessment of the bacterial flora of the proximal part of the small intestine in healthy cats, and the effect of sample collection method. Am. J. Vet. Res. 59:48-51.
19. Paster, B. J., S. K. Boches, J. L. Galvin, R. E. Ericson, C. N. Lau, V. A. Levanos, A. Sahasrabudhe, and F. E. Dewhirst. 2001. Bacterial diversity in human subgingival plaque. J. Bacteriol. 183:3770-3783.
20. Qiu, X., L. Wu, H. Huang, P. E. McDonel, A. V. Palumbo, J. M. Tiedje, and J. Zhou. 2001. Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning. Appl. Environ. Microbiol. 67:880-887.
21. Rutgers, H. C., R. M. Batt, F. J. Proud, S. H. Sorensen, C. M. Elwood, G. Petrie, L. A. Matthewman, H. A. F. Van Hijfte, A. Boswood, M. Entwistle, and R. H. Fensome. 1996. Intestinal permeability and function in dogs with small intestinal bacterial overgrowth. J. Small Anim. Pract. 37:428-434.
22. Simpson, J. M., B. Martineau, W. E. Jones, J. M. Ballam, and R. I. Mackie. 2002. Characterization of fecal bacterial populations in canines: effects of age, breed and dietary fiber. Microb. Ecol. 44:186-197.
23. Simpson, J. M., V. J. McCracken, H. R. Gaskins, and R. I. Mackie. 2000. Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53. Appl. Environ. Microbiol. 66:4705-4714.
24. Simpson, J. M., V. J. McCracken, B. A. White, H. R. Gaskins, and R. I. Mackie. 1999. Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota. J. Microbiol. Methods 36:167-179.
25. Suau, A., R. Bonnet, M. Sutren, J. J. Godon, G. R. Gibson, M. D. Collins, and J. Dore. 1999. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65:4799-4807.
26. Temmerman, R., L. Masco, T. Vanhoutte, G. Huys, and J. Swings. 2003. Development and validation of a nested-PCR-denaturing gradient gel electrophoresis method for taxonomic characterization of bifidobacterial communities. Appl. Environ. Microbiol. 69:6380-6385.
27. Willard, M. D., R. B. Simpson, E. K. Delles, N. D. Cohen, T. W. Fossum, D. Kolp, and G. Reinhart. 1994. Effects of dietary supplementation of fructo-oligosaccharides on small intestinal bacterial overgrowth in dogs. Am. J. Vet. Res. 55:654-659.
28. Willard, M. D., R. B. Simpson, T. W. Fossum, N. D. Cohen, E. K. Delles, D. L. Kolp, D. P. Carey, and G. A. Reinhart. 1994. Characterization of naturally developing small intestinal bacterial overgrowth in 16 German shepherd dogs. J. Am. Vet. Med. Assoc. 204:1201-1206.