On-line multitasking analytical proteomics: How to separate, reduce, alkylate and digest whole proteins in an on-line multidimensional chromatography system coupled to MS

Journal of Separation Science

Volumn 31, Issue 16-17, 2008, Pages 2913-2923

  Tran, Bao Quoc ^a   Loftheim, Håvard ^a   Reubsaet, Leon ^a   Lundanes, Elsa ^a   Greibrokk, Tyge ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

Multidimensional LC; On column reduction and alkylation; Online tryptic digestion; pH gradient; Protein separation

Indexed keywords

ALKYLATION; ANIMAL CELL CULTURE; CHEMICAL REACTIONS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; HYDROCARBONS; ION EXCHANGE; ISOMERS; MOLECULAR BIOLOGY; PROTEINS; SEPARATION;

ANALYTICAL PROCEDURES; COLUMN METHODS; ELECTROSPRAY; HUMAN URINE SAMPLES; MODEL PROTEINS; MS DETECTIONS; MULTIDIMENSIONAL CHROMATOGRAPHIES; MULTIDIMENSIONAL LC; MULTIDIMENSIONAL SYSTEMS; NATIVE PROTEINS; NATIVE STATES; ON-COLUMN REDUCTION AND ALKYLATION; ON-LINE; ONLINE TRYPTIC DIGESTION; PACKED CAPILLARY COLUMNS; PH GRADIENTS; PROTEIN SEPARATION; PROTEOMICS; STRONG ANION EXCHANGES; TRYPTIC DIGESTIONS;

COLUMNS (STRUCTURAL);

ALKYLATION; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; ONLINE ANALYSIS; ONLINE SYSTEM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; REDUCTION; URINALYSIS;

ALKYLATION; ANIMALS; CATTLE; CHICKENS; CHROMATOGRAPHY, LIQUID; CYTOCHROMES C; ENZYMES, IMMOBILIZED; HORSES; HUMANS; HYDROGEN-ION CONCENTRATION; LACTOGLOBULINS; MURAMIDASE; MYOGLOBIN; OXIDATION-REDUCTION; PROTEINS; PROTEOMICS; SENSITIVITY AND SPECIFICITY; SERUM ALBUMIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN; URINE;

EID: 54349123070     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200800275     Document Type: Article

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