Structural and thermodynamic characterization of T4 lysozyme mutants and the contribution of internal cavities to pressure denaturation

Biochemistry

Volumn 47, Issue 42, 2008, Pages 11097-11109

  Ando, Nozomi ^a   Barstow, Buz ^a   Baase, Walter A ^c   Fields, Andrew ^c   Matthews, Brian W ^c   Gruner, Sol M ^a,b,d  

^a Cornell University   (United States)

^b Department of Obstetrics Gynecology   (United States)

^c UNIVERSITY OF OREGON   (United States)

^d NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENZYMES; HYDRATES; OFFSHORE OIL WELL PRODUCTION; POWDERS; X RAY SCATTERING;

CAVITY VOLUMES; CHEMICAL DENATURATIONS; COLLINS; DENATURED STATES; FOLDED STATES; HIGH PRESSURES; HYDROPHOBIC RESIDUES; INTERNAL CAVITIES; NEUTRAL PHS; PRESSURE DENATURATIONS; RADIUS OF GYRATIONS; SOLUTION BEHAVIORS; T4 LYSOZYMES; VOLUME CHANGES; WATER PENETRATIONS;

DENATURATION;

LYSOZYME; WATER;

ARTICLE; CORRELATION ANALYSIS; CRYSTAL STRUCTURE; DENATURATION; ENZYME STABILITY; ENZYME STRUCTURE; PH; PRIORITY JOURNAL; RADIUS; STRUCTURE ANALYSIS; THERMODYNAMICS; WATER PERMEABILITY;

AMINO ACID SUBSTITUTION; BACTERIOPHAGE T4; CRYSTALLOGRAPHY, X-RAY; HYDROGEN-ION CONCENTRATION; MURAMIDASE; MUTAGENESIS, SITE-DIRECTED; PRESSURE; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SCATTERING, SMALL ANGLE; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; TRYPTOPHAN; WATER; X-RAY DIFFRACTION;

EID: 54349114270     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801287m     Document Type: Article

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