Specific detection and quantification of culturable and non-culturable mycobacteria in metalworking fluids by fluorescence-based methods

Letters in Applied Microbiology

Volumn 47, Issue 5, 2008, Pages 451-456

  Selvaraju, S B ^a,b   Khan, I U H ^a,c   Yadav, J S ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^b CHILDREN S MERCY HOSPITAL   (United States)

^c ENVIRONMENT CANADA   (Canada)

Author keywords

Epifluorescence microscopy; Live Dead BacLight staining; Metalworking fluid; Mycobacterium immunogenum; Non culturable; Real time PCR; Viable but nonculturable

Indexed keywords

BACTERIA; FLUIDS; FLUORESCENCE MICROSCOPY; METAL WORKING; POLYMERASE CHAIN REACTION;

AURAMINE; CULTURABLE; EPIFLUORESCENCE MICROSCOPY; LIVE/DEAD BACLIGHT STAINING; METALWORKING FLUIDS; MYCOBACTERIUM IMMUNOGENUM; NON-CULTURABLE; REAL-TIME PCR; RHODAMINE; VIABLE BUT NON-CULTURABLE;

FLUORESCENCE;

AGAR; AURAMINE; METAL; RHODAMINE;

BACTERIA; FLUORESCENCE; OPTIMIZATION;

BACTERIUM; DETECTION METHOD; FLUID; FLUORESCENCE; HAZARD ASSESSMENT; OCCUPATIONAL EXPOSURE; OPTIMIZATION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; VIABILITY;

ARTICLE; BACTERIAL COUNT; BACTERIAL FLORA; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; MYCOBACTERIUM; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM IMMUNOGENUM;

EID: 54249110956     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2008.02419.x     Document Type: Article

References (16)

1. Bernstein, D.I., Lummus, Z.L., Santilli, G., Siskosky, J. Bernstein, I.L. (1995) Machine operator's lung. A hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. Chest 108, 636 641.
2. Besnard, V., Federighi, M. Cappelier, J.M. (2000) Development of direct viable count procedure for the investigation of VBNC state in Listeria monocytogenes. Lett Appl Microbiol 31, 77 81.
3. Boulos, L., Prevost, M., Barbeau, B., Coallier, J. Desjardins, R. (1999) LIVE/DEAD BacLight: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Methods 37, 77 86.
4. CDC (2001) Respiratory illness in workers exposed to metalworking fluid contaminated with nontuberculous mycobacteria-ohio, 2001. MMWR 51, 349 352.
5. Duffy, G., Kilbride, B., Sheridan, J.J., Blair, I.S. McDowell, D.A. (2000) A membrane-immunofluorescence-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples. J Appl Microbiol 89, 587 594.
6. Joux, F., Lebaron, P. Troussellier, M. (1997) Succession of cellular states in a Salmonella typhimurium population during starvation in artificial seawater microcosms. FEMS Microbiol Ecol 22, 65 76.
7. Khan, I.U.H. Yadav, J.S. (2004a) Real-time PCR assays for genus-specific detection and quantification of culturable and non-culturable mycobacteria and pseudomonads in metalworking fluids. Mol Cell Probes 18, 67 73.
8. Khan, I.U.H. Yadav, J.S. (2004b) Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis. J Clin Microbiol 42, 453 457.
9. Kreiss, K. Cox-Ganser, J. (1997) Metal working fluid-associated hypersensitivity pneumonitis: a workshop summary. Am J Ind Med 32, 423 432.
10. Mattsby-Baltzer, I., Sandin, M., Ahlstrom, B., Allenmark, S., Edebo, M., Falsen, E., Pedersen, K., Rodin, N. et al. (1989) Microbial growth and accumulation in industrial metal-working fluids. Appl Environ Microbiol 55, 2681 2689.
11. Selvaraju, S.B., Khan, I.U.H. Yadav, J.S. (2005a) A new method for species identification and differentiation of Mycobacterium chelonae complex based on Amplified hsp65 Restriction Analysis (AHSPRA). Mol Cell Probes 19, 93 99.
12. Selvaraju, S.B., Khan, I.U.H. Yadav, J.S. (2005b) Biocidal activity of formaldehyde and nonformaldehyde biocides toward Mycobacterium immunogenum and Pseudomonas fluorescens in pure and mixed suspensions in synthetic metalworking fluid and saline. Appl Environ Microbiol 71, 542 546.
13. Somoskovi, A., Hotaling, J.E., Fitzgerald, M., O'Donnell, D., Parson, L.M. Safinger, M. (2001) Lessons from a proficiency testing event for acid-fast microscopy. Chest 120, 250 257.
14. Tenover, F.C., Crawford, J.T., Huebner, R.E., Geiter, L.J., Horsburgh, C.R. Jr. Good, R.C. (1993) The resurgence of tuberculosis: is your laboratory ready? J Clin Microbiol 31, 767 770.
15. Yadav, J.S., Khan, I.U.H., Fakhari, F. Soellner, M.B. (2003) DNA-based methodologies for rapid detection, quantification, and species- or strain-level identification of respiratory pathogens (Mycobacteria and Pseudomonads) in metalworking fluids. Appl Occup Environ Hyg 18, 966 975.
16. Yadav, J.S., Selvaraju, S.B. Khan, I.U.H. (2006) Enhanced recovery and real-time PCR-based quantification of mycobacteria from metalworking fluids. J ASTM Int 3, 1 18.