Fluorescence confocal laser scanning microscopy for in vivo imaging of epidermal reactions to two experimental irritants

Skin Research and Technology

Volumn 14, Issue 4, 2008, Pages 498-503

  Suihko, Christian ^a,c   Serup, Jørgen ^a,b  

^a LINKÖPING UNIVERSITY HOSPITAL   (Sweden)

^b BISPEBJERG HOSPITAL   (Denmark)

^c LINKÖPING UNIVERSITY   (Sweden)

Author keywords

Confocal laser scanning microscopy; Irritant contact dermatitis; Keratinocytes; Pelargonic acid; Sodiumlauryl sulphate

Indexed keywords

ACIDS; CELL MEMBRANES; CELL PROLIFERATION; CELLS; CHEMICAL PROPERTIES; CONFOCAL MICROSCOPY; CYTOLOGY; DISSOLUTION; EXPERIMENTS; FLUORESCENCE; IMAGING TECHNIQUES; LASER APPLICATIONS; LASERS; LIGHT EMISSION; LUMINESCENCE; OPTICAL SYSTEMS; POWER AMPLIFIERS; SCANNING; SKIN; SODIUM;

CONFOCAL LASER SCANNING MICROSCOPY; IRRITANT CONTACT DERMATITIS; KERATINOCYTES; PELARGONIC ACID; SODIUMLAURYL SULPHATE;

MICROSCOPIC EXAMINATION;

2 PROPANOL; DODECYL SULFATE SODIUM; NONANOIC ACID;

ADULT; ARTICLE; CELL DAMAGE; CELL MEMBRANE; CELL PROLIFERATION; CONFOCAL LASER MICROSCOPY; FEMALE; FLUORESCENCE CONFOCAL LASER SCANNING MICROSCOPY; HUMAN; HUMAN EXPERIMENT; IMAGE ANALYSIS; IMAGE DISPLAY; IN VIVO STUDY; IRRITANT DERMATITIS; KERATINOCYTE; MALE; NORMAL HUMAN; PARAKERATOSIS; PHYSICAL CHEMISTRY; SKIN EXAMINATION; SKIN SURFACE;

ADOLESCENT; ADULT; DERMATITIS, IRRITANT; DERMOSCOPY; EPIDERMIS; FEMALE; HUMANS; IRRITANTS; MALE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 54049150401     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/j.1600-0846.2008.00323.x     Document Type: Article

References (11)

1. Gonzalez S, Gilaberte-Calzada Y, Jáen-Olasold P, Rajadhyaksha M, Torres A, Halpern A. In vivo reflectance mode confocal microscopy in clinical and surgical dermatology. In: Serup J, Jemec G, Grove G, eds. Handbook of noninvasive methods and the skin, 2nd edn. Boca Raton: CRC Press, 2006: 267-276.
2. Ogoshi K, Serup J. Safety of sodium fluorescein for in vivo study of skin. Skin Res Technol 2006; 12: 155-161.
3. Haugland RP. Thiolreactive probes. In: Gregory J, ed. Handbook of fluorescent probes and research chemicals, 9th edn. Eugene, OR: Molecular Probes Inc, 2002: 46-50.
4. Zhai H, Maibach HI. Occlusion vs. skin barrier function. Skin Res Technol 2002; 8: 1-6.
5. Papworth GD, Delaney PM, Bussau LJ, Vo LT, King RG. In vivo fibre-optic confocal imaging of microvasculature and nerves in the rat vas deferens and colon. J Anat 1998; 192: 489-495.
6. Swindle LD, Thomas SG, Freeman M, Delaney PM. View of normal skin in vivo as observed using fluorescent fibre-optic confocal microscopic imaging. J Invest Dermatol 2003; 121: 706-712.
7. Suihko C, Swindle LD, Thomas SG, Serup J. Fluorescence fibre-optic confocal microscopy of skin in vivo: Microscope and fluorophores. Skin Res Technol 2005; 11: 254-267.
8. Tupker RA, Willis C, Berardesca E, Lee CH, Fartasch M, Agner T, Serup J. Guidelines on sodium lauryl sulphate (SLS) exposure tests. A report from the Standardization Group of the European Society for Contact Dermatitis. Contact Dermatitis 1997; 37: 53-69.
9. Wahlberg GE, Lindberg M. Nonanoic acid -an experimental irritant. Contact Dermatitis 2003; 49: 117-123.
10. Frosch PJ, Pilz B. Irritant patch test techniques. In: Serup J, Jemec GBE, eds. Handbook of non-invasive methods and the skin. Boca Raton: CRC Press Inc, 1995: 587-591.
11. Delaney PM, Harris MR, King RG. Fibre-optic laser scanning confocal microscope suitable for fluorescence imaging. Appl Optics 1994; 33: 573-577.