Overexpression of CART in the PVN increases food intake and weight gain in rats

Obesity

Volumn 16, Issue 10, 2008, Pages 2239-2244

  Smith, Kirsty L ^a   Gardiner, James V ^a   Ward, Helen L ^a   Kong, Wing M ^a   Murphy, Kevin G ^a   Martin, Niamh M ^a   Ghatei, Mohammad A ^a   Bloom, Stephen R ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PEPTIDE; ION CHANNEL; LEPTIN; MESSENGER RNA; MITOCHONDRIAL PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN; NERVE PROTEIN; NEUROPEPTIDE; THYROTROPIN;

ADENO ASSOCIATED VIRUS; ADIPOSE TISSUE; ANIMAL; ANIMAL BEHAVIOR; ANTERIOR HYPOTHALAMUS; ARTICLE; BLOOD; EATING; FAT INTAKE; FOOD INTAKE; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; MALE; METABOLISM; RAT; TIME; UPREGULATION; WEIGHT GAIN; WISTAR RAT;

ADIPOSE TISSUE; ANIMALS; APPETITE REGULATION; BEHAVIOR, ANIMAL; DEPENDOVIRUS; DIETARY FATS; EATING; GENETIC VECTORS; ION CHANNELS; LEPTIN; MALE; MITOCHONDRIAL PROTEINS; NERVE TISSUE PROTEINS; NEUROPEPTIDES; PARAVENTRICULAR HYPOTHALAMIC NUCLEUS; RATS; RATS, WISTAR; RNA, MESSENGER; THYROTROPIN; TIME FACTORS; TRANSDUCTION, GENETIC; UP-REGULATION; WEIGHT GAIN;

ADENO-ASSOCIATED VIRUS; ANIMALIA; RATTUS; RATTUS NORVEGICUS;

EID: 53849132680     PISSN: 19307381     EISSN: 1930739X     Source Type: Journal    
DOI: 10.1038/oby.2008.366     Document Type: Article

References (40)

1. Douglass J, McKinzie AA, Couceyro P. PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine. J Neurosci 1995;15:2471-2481.
2. Douglass J, Daoud S. Characterization of the human cDNA and genomic DNA encoding CART: a cocaine- and amphetamine-regulated transcript. Gene 1996;169:241-245.
3. Gautvik KM, de Lecea L, Gautvik VT et al. Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction. Proc Natl Acad Sci USA 1996;93:8733-8738.
4. Couceyro PR, Koylu EO, Kuhar MJ. Further studies on the anatomical distribution of CART by in situ hybridization. J Chem Neuroanat 1997;12:229-241.
5. Vrang N, Tang-Christensen M, Larsen PJ, Kristensen P. Recombinant CART peptide induces c-Fos expression in central areas involved in control of feeding behaviour. Brain Res 1999;818:499-509.
6. Kalra SP, Dube mg, Pu S et al. Interacting appetite-regulating pathways in the hypothalamic regulation of body weight. Endocr Rev 1999;20:68-100.
7. Kristensen P, Judge ME, Thim L et al. Hypothalamic CART is a new anorectic peptide regulated by leptin. Nature 1998;393:72-76.
8. Jensen PB, Kristensen P, Clausen JT et al. The hypothalamic satiety peptide CART is expressed in anorectic and non-anorectic pancreatic islet tumors and in the normal islet of Langerhans. FEBS Lett 1999;447:139-143.
9. Stanley SA, Small CJ, Murphy KG et al. Actions of cocaine- and amphetamine-regulated transcript (CART) peptide on regulation of appetite and hypothalamo-pituitary axes in vitro and in vivo in male rats. Brain Res 2001;893:186-194.
10. Asakawa A, Inui A, Yuzuriha H et al. Cocaine-amphetamine-regulated transcript influences energy metabolism, anxiety and gastric emptying in mice. Horm Metab Res 2001;33:554-558.
11. Qing K, Chen Y. Central CART gene delivery by recombinant AAV vector attenuates body weight gain in diet-induced-obese rats. Regul Pept 2007;140:21-26.
12. Abbott CR, Rossi M, Wren AM et al. Evidence of an orexigenic role for cocaine- and amphetamine-regulated transcript after administration into discrete hypothalamic nuclei. Endocrinology 2001;142:3457-3463.
13. Kong WM, Stanley S, Gardiner J et al. A role for arcuate cocaine and amphetamine-regulated transcript in hyperphagia, thermogenesis, and cold adaptation. FASEB J 2003;17:1688-1690.
14. Larsen PJ, Vrang N, Petersen PC, Kristensen P. Chronic intracerebro-ventricular administration of recombinant CART(42-89) peptide inhibits and causes weight loss in lean and obese Zucker (fa/fa) rats. Obes Res 2000;8:590-596.
15. Antin J, Gibbs J, Holt J, Young RC, Smith GP. Cholecystokinin elicits the complete behavioral sequence of satiety in rats. J Comp Physiol Psychol 1975;89:784-790.
16. Aja S, Schwartz GJ, Kuhar MJ, Moran TH. Intracerebroventricular CART peptide reduces rat ingestive behavior and alters licking microstructure. Am J Physiol Regul Integr Comp Physiol 2001;280:R1613-R1619.
17. Aja S, Sahandy S, Ladenheim EE, Schwartz GJ, Moran TH. Intracerebro-ventricular CART peptide reduces food intake and alters motor behavior at a hindbrain site. Am J Physiol Regul Integr Comp Physiol 2001;281:R1862-R1867.
18. Kaplitt mg, Leone P, Samulski RJ et al. Long-term gene expression and phenotypic correction using adeno-associated virus vectors in the mammalian brain. Nat Genet 1994;8:148-154.
19. Bartlett JS, Samulski RJ, McCown TJ. Selective and rapid uptake of adeno-associated virus type 2 in brain. Hum Gene Ther 1998;9:1181-1186.
20. Klein RL, Hamby ME, Gong Y et al. Dose and promoter effects of adeno-associated viral vector for green fluorescent protein expression in the rat brain. Exp Neurol 2002;176:66-74.
21. Lundberg C, Jungles SJ, Mulligan RC. Direct delivery of leptin to the hypothalamus using recombinant adeno-associated virus vectors results in increased therapeutic efficacy. Nat Biotechnol 2001;19:169-172.
22. Li G, Mobbs CV, Scarpace PJ. Central pro-opiomelanocortin gene delivery results in hypophagia, reduced visceral adiposity, and improved insulin sensitivity in genetically obese Zucker rats. Diabetes 2003;52:1951-1957.
23. Gardiner JV, Kong WM, Ward H et al. AAV mediated expression of anti-sense neuropeptide Y cRNA in the arcuate nucleus of rats results in decreased weight gain and food intake. Biochem Biophys Res Commun 2005;327:1088-1093.
24. Grimm D, Kern A, Rittner K, Kleinschmidt JA. Novel tools for production and purification of recombinant adenoassociated virus vectors. Hum Gene Ther 1998;9:2745-2760.
25. Zolotukhin S, Byrne BJ, Mason E et al. Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield. Gene Ther 1999;6:973-985.
26. Paxinos G, Watson C. The Rat Brain in Stereotactic Co-ordinates, 4th edn. Academic Press: London, 1998.
27. Dhillo WS, Small CJ, Stanley SA et al. Hypothalamic interactions between neuropeptide Y, agouti-related protein, cocaine-and amphetamine- regulated transcript and alpha-melanocyte-stimulating hormone in vitro in male rats. J Neuroendocrinol 2002;14:725-730.
28. Paterna JC, Moccetti T, Mura A, Feldon J, Bueler H. Influence of promoter and WHV post-transcriptional regulatory element on AAV-mediated transgene expression in the rat brain. Gene Ther 2000;7:1304-1311.
29. Bewick GA, Dhillo WS, Darch SJ et al. Hypothalamic cocaine- and amphetamine-regulated transcript (CART) and agouti-related protein (AgRP) neurons coexpress the NOP1 receptor and nociceptin alters CART and AgRP release. Endocrinology 2005;146:3526-3534.
30. Beak SA, Small CJ, Ilovaiskaia I et al. Glucagon-like peptide-1 (GLP-1) releases thyrotropin (TSH): characterization of binding sites for GLP-1 on alpha-TSH cells. Endocrinology 1996;137:4130-4138.
31. Kong WM, Martin NM, Smith KL et al. Triiodothyronine stimulates food intake via the hypothalamic ventromedial nucleus independent of changes in energy expenditure. Endocrinology 2004;145:5252-5258.
32. Fisher KJ, Gao GP, Weitzman MD et al. Transduction with recombinant adeno-associated virus for gene therapy is limited by leading-strand synthesis. J Virol 1996;70:520-532.
33. Ward H, Gardiner J, Kong WM, Murphy K, Bloom S. Distribution of fluorescence following injection of recombinant adeno-associated virus encoding green fluorescent protein into the paraventricular nucleus. Neuroendocrinology 2003;77:100-104.
34. Koylu EO, Couceyro PR, Lambert PD, Kuhar MJ. Cocaine- and amphetamine-regulated transcript peptide immunohistochemical localization in the rat brain. J Comp Neurol 1998;391:115-132.
35. Zheng H, Patterson C, Berthoud HR. Fourth ventricular injection of CART peptide inhibits short-term sucrose intake in rats. Brain Res 2001;896:153-156.
36. Zheng H, Patterson LM, Berthoud HR. CART in the dorsal vagal complex: sources of immunoreactivity and effects on Fos expression and food intake. Brain Res 2002;957:298-310.
37. Elias CF, Lee C, Kelly J et al. Leptin activates hypothalamic CART neurons projecting to the spinal cord. Neuron 1998;21:1375-1385.
38. Cowley MA, Smart JL, Rubinstein M et al. Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus. Nature 2001;411:480-484.
39. Mounien L, Bizet P, Boutelet I, Vaudry H, Jegou S. Expression of melanocortin MC3 and MC4 receptor mRNAs by neuropeptide Y neurons in the rat arcuate nucleus. Neuroendocrinology 2005;82:164-170.
40. Hahn TM, Breininger JF, Baskin DG, Schwartz MW. Coexpression of Agrp and NPY in fasting-activated hypothalamic neurons. Nat Neurosci 1998;1:271-272.