Small molecule-induced allosteric activation of the Vibrio cholerae RTX cysteine protease domain

Science

Volumn 322, Issue 5899, 2008, Pages 265-268

  Lupardus, Patrick J ^a   Shen, Aimee ^a   Bogyo, Matthew ^a   Garcia, K Christopher ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CASPASE 7; CHOLERA TOXIN; CYSTEINE PROTEINASE; GINGIPAIN R; PHYTIC ACID; UNCLASSIFIED DRUG; VIBRIO CHOLERAE RTX TOXIN;

BIOCHEMISTRY; ENZYME ACTIVITY; MUTATION; PROTEIN; REACTION KINETICS; TOXIN;

ALLOSTERISM; ARTICLE; BINDING KINETICS; COMPLEX FORMATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; EUKARYOTE; GENE MUTATION; INTRACELLULAR TRANSPORT; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROCESSING; VIBRIO CHOLERAE;

ACYLTRANSFERASES; ALLOSTERIC REGULATION; BACTERIAL PROTEINS; BACTERIAL TOXINS; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CYSTEINE ENDOPEPTIDASES; ENZYME ACTIVATION; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HYDROGEN BONDING; MODELS, MOLECULAR; PHYTIC ACID; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; SURFACE PLASMON RESONANCE; VIBRIO CHOLERAE;

EUKARYOTA; VIBRIO CHOLERAE;

EID: 53749083462     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1162403     Document Type: Article

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17. We thank S. Juo for assistance with data collection and structure determination, E. D. Sandoval for assistance with kinetic analyses and helpful discussion, M. Blokech and G. Schoolnik for help with V. cholerae strain construction, and A. Guzzetta for intact mass analysis of SeMet-labeled CPD. P.J.L. is a Damon Runyon Fellow, supported by the Damon Runyon Cancer Research Foundation. K.C.G. is supported by the NIH Keck Foundation and the Howard Hughes Medical Institute. M.B. is supported by the Burroughs Wellcome Foundation, the Searle Scholars Program, and the NIH National Technology Center for Networks and Pathways (grant U54RR020843). P.J.L, A.S., M.B., and K.C.G. are listed as inventors on a patent application related to use of the V. cholerae RTX CPD for biotechnical applications. Coordinates and structure factors have been deposited in the Protein Data Bank (www.rcsb.org) under accession number 3EEB.