Principles and applications of polymerase chain reaction: Basic science for the practicing physician

Annals of Allergy, Asthma and Immunology

Volumn 101, Issue 4, 2008, Pages 437-443

  Ishmael, Faoud T ^a   Stellato, Cristiana ^a,b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b JOHNS HOPKINS ASTHMA AND ALLERGY CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ADOLESCENT; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; ETHICS; FEMALE; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC MANIPULATION; HUMAN; MALE; MEDICAL PRACTICE; MEDICAL RESEARCH; MOLECULAR CLONING; PHYSICIAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; SCIENTIFIC LITERATURE;

EID: 53549112792     PISSN: 10811206     EISSN: 15344436     Source Type: Journal    
DOI: 10.1016/S1081-1206(10)60323-7     Document Type: Article

References (20)

1. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335-350.
2. Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990;262:56-61, 64-55.
3. Olson M, Hood L, Cantor C, Botstein D. A common language for physical mapping of the human genome. Science. 1989;245:1434-1435.
4. Panet A, Khorana HG. Studies on polynucleotides. the linkage of deoxyribopolynucleotide templates to cellulose and its use in their replication. J Biol Chem. 1974;249:5213-5221.
5. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques. 1997;22:130-131, 134-138.
6. Palmer S, Wiegand AP, Maldarelli F, et al. New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol. 2003;41:4531-4536.
7. Saiki RK, Scharf S, Faloona F, et al. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230:1350-1354.
8. Hentemann M, Reiss J, Wagner M, Cooper DN. Rapid detection of deletions in the Duchenne muscular dystrophy gene by PCR amplification of deletion-prone exon sequences. Hum Genet. 1990;84:228-232.
9. Hensel CH, Xiang RH, Sakaguchi AY, Naylor SL. Use of the single strand conformation polymorphism technique and PCR to detect p53 gene mutations in small cell lung cancer. Oncogene. 1991;6:1067-1071.
10. McGee TL, Cowley GS, Yandell DW, Dryja TP. Detection of the XbaI RFLP within the retinoblastoma locus by PCR. Nucleic Acids Res. 1990;18:207.
11. Tamaki K, Jeffreys AJ. Human tandem repeat sequences in forensic DNA typing. Leg Med (Tokyo). 2005;7:244-250.
12. Tang YW, Procop GW, Persing DH. Molecular diagnostics of infectious diseases. Clin Chem. 1997;43:2021-2038.
13. Mackay IM. Real-time PCR in the microbiology laboratory. Clin Microbiol Infect. 2004;10:190-212.
14. Wong ML, Medrano JF. Real-time PCR for mRNA quantitation. Biotechniques. 2005;39:75-85.
15. Valasek MA, Repa JJ. The power of real-time PCR. Adv Physiol Educ. 2005;29:151-159.
16. Gibson UE, Heid CA, Williams PM. A novel method for real time quantitative RT-PCR. Genome Res. 1996;6:995-1001.
17. Clegg RM, Murchie AI, Zechel A, Carlberg C, Diekmann S, Lilley DM. Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction. Biochemistry. 1992;31:4846-4856.
18. Tichopad A, Dilger M, Schwarz G, Pfaffl MW. Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res. 2003;31:e122.
19. Kuhne BS, Oschmann P. Quantitative real-time RT-PCR using hybridization probes and imported standard curves for cytokine gene expression analysis. Biotechniques. 2002;33:1078, 1080-1082, 1084 passim.
20. Baliga CS, Sutton RE. The role of phenotyping and replication capacity in anti-HIV therapeutics. Curr Opin Mol Ther. 2004;6:308-317.