Membrane-association determinants of the ω-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa

Microbiology

Volumn 154, Issue 9, 2008, Pages 2804-2813

  Imperi, Francesco ^a,b   Putignani, Lorenzo ^b,c   Tiburzi, Federica ^a,b   Ambrosi, Cecilia ^a,d   Cipollone, Rita ^a,e   Ascenzi, Paolo ^a,b   Visca, Paolo ^a,b  

^a ROMA TRE UNIVERSITY   (Italy)

^b NATIONAL INSTITUTE FOR INFECTIOUS DISEASES L SPALLANZANI   (Italy)

^c BAMBINO GESÙ CHILDREN S HOSPITAL   (Italy)

^d CNR   (Italy)

^e UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; OMEGA AMINO ACID MONOOXYGENASE PVDA; PYOVERDINE; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; MIXED FUNCTION OXIDASE; OLIGOPEPTIDE; ORNITHINE; ORNITHINE HYDROXYLASE, PSEUDOMONAS AERUGINOSA;

ARTICLE; BACTERIUM MUTANT; BINDING SITE; CELL FRACTIONATION; CONTROLLED STUDY; CYTOSOL; ENZYME BINDING; ENZYME LOCALIZATION; ENZYME STRUCTURE; HYDROPHOBICITY; MATHEMATICAL COMPUTING; MEMBRANE STRUCTURE; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; PSEUDOMONAS AERUGINOSA; BACTERIAL GENE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE DELETION; GENETIC COMPLEMENTATION; GENETICS; METABOLISM; MOLECULAR CLONING; POINT MUTATION; PROTEIN SECONDARY STRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

ALKALINE PHOSPHATASE; BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; HYDROPHOBICITY; MEMBRANE PROTEINS; MIXED FUNCTION OXYGENASES; OLIGOPEPTIDES; ORNITHINE; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS;

EID: 53449085207     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.2008/018804-0     Document Type: Article

References (41)

1. Akers, H. A. & Neilands, J. B. (1973). A hydroxamic acid present in Rhodotorula pilimanae cultures grown at low pH and its metabolic relation to rhodotorulic acid. Biochemistry 12, 1006-1010.
2. Andrews, S. C., Robinson, A. K. & Rodriguez-Quinones, F. (2003). Bacterial iron homeostasis. FEMS Microbiol Rev 27, 215-237.
3. Arts, J., van Boxtel, R., Filloux, A., Tommassen, J. & Koster, M. (2007). Export of the pseudopilin XcpT of the Pseudomonas aeruginosa type II secretion system via the signal recognition particle-Sec pathway. J Bacteriol 189, 2069-2076.
4. Bleves, S., Lazdunski, A. & Filloux, A. (1996). Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa. J Bacteriol 178, 4297-4300.
5. Deber, C. M., Liu, L. P. & Wang, C. (1999). Perspective: peptides as mimics of transmembrane segments in proteins. J Pept Res 54, 200-205.
6. Dick, S., Marrone, L., Thariath, A., Valvano, M. A. & Viswanatha, T. (1998). Cofactor- and substrate-binding domains in flavin-dependent N-hydroxylating enzymes. Trends Biochem Sci 23, 414-415.
7. Dym, O. & Eisenberg, D. (2001). Sequence-structure analysis of FAD-containing proteins. Protein Sci 10, 1712-1728.
8. Filip, C., Fletcher, G., Wulff, J. L. & Earhart, C. F. (1973). Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol 115, 717-722.
9. 5-hydroxylase involved in pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa. J Bacteriol 188, 7205-7210.
10. Goder, V. & Spiess, M. (2001). Topogenesis of membrane proteins: Determinants and dynamics. FEBS Lett 504, 87-93.
11. Guilvout, I., Carniel, E. & Pugsley, A. P. (1995). Yersinia spp. HMWP2, a cytosolic protein with a cryptic internal signal sequence which can promote alkaline phosphatase export. J Bacteriol 177, 1780-1787.
12. Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177, 4121-4130.
13. Hantash, F. M. & Earhart, C. F. (2000). Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF. J Bacteriol 182, 1768-1773.
14. Herrero, M., de Lorenzo, V. & Neilands, J. B. (1988). Nucleotide sequence of the iucD gene of the pColV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions. J Bacteriol 170, 56-64.
15. Hoang, T. T., Karkhoff-Schweizer, R. R., Kutchma, A. J. & Schweizer, H. P. (1998). A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally located DNA sequences: Application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212, 77-86.
16. Hopp, T. P. & Woods, K. R. (1983). A computer program for predicting protein antigenic determinants. Mol Immunol 20, 483-489.
17. Kyte, J. & Doolittle, R. F. (1982). A simple method for displaying the hydropathic character of a protein. J Mol Biol 157, 105-132.
18. Landolt-Marticorena, C., Williams, K. A., Deber, C. M. & Reithmeier, R. A. (1993). Non-random distribution of amino acids in the transmembrane segments of human type I single span membrane proteins. J Mol Biol 229, 602-608.
19. Li, S. C., Goto, N. K., Williams, K. A. & Deber, C. M. (1996). α-Helical, but not β-sheet, propensity of proline is determined by peptide environment. Proc Natl Acad Sci U S A 93, 6676-6681.
20. Manoil, C., Mekalanos, J. J. & Beckwith, J. (1990). Alkaline phosphatase fusions: Sensors of subcellular location. J Bacteriol 172, 515-518.
21. Melchers, K., Schuhmacher, A., Buhmann, A., Weitzenegger, T., Belin, D., Grau, S. & Ehrmann, M. (1999). Membrane topology of CadA homologous P-type ATPase of Helicobacter pylori as determined by expression of phoA fusions in Escherichia coli and the positive inside rule. Res Microbiol 150, 507-520.
22. Meneely, K. M. & Lamb, A. L. (2007). Biochemical characterization of a flavin adenine dinucleotide-dependent monooxygenase, ornithine hydroxylase from Pseudomonas aeruginosa, suggests a novel reaction mechanism. Biochemistry 46, 11930-11937.
23. Michaelis, S., Inouye, H., Oliver, D. & Beckwith, J. (1983). Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli. J Bacteriol 154, 366-374.
24. Michel, G., Bleves, S., Ball, G., Lazdunski, A. & Filloux, A. (1998). Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa. Microbiology 144, 3379-3386.
25. Neu, H. C. & Heppel, L. A. (1965). The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem 240, 3685-3692.
26. Plattner, H. J., Pfefferle, P., Romaguera, A., Waschutza, S. & Diekmann, H. (1989). Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222. Biol Met 2, 1-5.
27. Putignani, L., Ambrosi, C., Ascenzi, P. & Visca, P. (2004). Expression of L-ornithine Ndelta-oxygenase (PvdA) in fluorescent Pseudomonas species: An immunochemical and in silico study. Biochem Biophys Res Commun 313, 245-257.
28. Robles-Price, A., Wong, T. Y., Sletta, H., Valla, S. & Schiller, N. L. (2004). AlgX is a periplasmic protein required for alginate biosynthesis in Pseudomonas aeruginosa. J Bacteriol 186, 7369-7377.
29. Rosenberg, I. M. (2005). Protein Analysis and Purification: Benchtop Techniques, 2nd edn. Cambridge, MA: Birkhauser Boston.
30. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
31. Schweizer, H. P. (1991). Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97, 109-121.
32. Seth, O., Smau, L., Welte, W., Ghisla, S., Stehr, M., Diekmann, H. & Macheroux, P. (1998). A reply to Dick et al. Trends Biochem Sci 23, 414-415.
33. Stehr, M., Diekmann, H., Smau, L., Seth, O., Ghisla, S., Singh, M. & Macheroux, P. (1998). A hydrophobic sequence motif common to N-hydroxylating enzymes. Trends Biochem Sci 23, 56-57.
34. Thariath, A., Socha, D., Valvano, M. A. & Viswanatha, T. (1993). Construction and biochemical characterization of recombinant cytoplasmic forms of the IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster. J Bacteriol 175, 589-596.
35. Tiburzi, F., Imperi, F. & Visca, P. (2008). Intracellular levels and activity of PvdS, the major iron starvation sigma factor of Pseudomonas aeruginosa. Mol Microbiol 67, 213-227.
36. Visca, P., Serino, L. & Orsi, N. (1992). Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdine. J Bacteriol 174, 5727-5731.
37. Visca, P., Ciervo, A., Sanfilippo, V. & Orsi, N. (1993). Iron-regulated salicylate synthesis by Pseudomonas spp. J Gen Microbiol 139, 1995-2001.
38. 5-oxygenase in Pseudomonas aeruginosa. J Bacteriol 176, 1128-1140.
39. Visca, P., Imperi, F. & Lamont, I. L. (2007). Pyoverdine siderophores: From biogenesis to biosignificance. Trends Microbiol 15, 22-30.
40. von Heijne, G. (1992). Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J Mol Biol 225, 487-494.
41. Williams, K. A. & Deber, C. M. (1991). Proline residues in transmembrane helices: Structural or dynamic role? Biochemistry 30, 8919-8923.