Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 178, Issue 14, 1996, Pages 4297-4300

  Bleves, Sophie ^a   Lazdunski, Andrée ^a   Filloux, Alain ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL PROTEIN; BETA LACTAMASE; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL OUTER MEMBRANE; CONTROLLED STUDY; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION; PROTEIN TRANSPORT; PSEUDOMONAS AERUGINOSA;

EID: 0030013762     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.14.4297-4300.1996     Document Type: Article

References (21)

1. Akrim, M., M. Bally, G. Ball, J. Tommassen, H. Teerink, A. Filloux, and A. Lazdunski. 1993. Xcp-mediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression. Mol. Microbiol. 10:431-443.
2. Bally, M., A. Filloux, M. Akrim, G. Ball, A. Lazdunski, and J. Tommassen. 1992. Processing of components of the secretory apparatus of Pseudomonas aeruginosa by prepilin peptidase. Mol. Microbiol. 6:1121-1131.
3. Broome-Smith, J., and B. G. Spratt. 1986. A vector for the construction of translational fusions to the TEM β-lactamase and analysis of protein export signals and membrane protein topology. Gene 49:341-349.
4. de Groot, A., A. Filloux, and J. Tommassen. 1991. Conservation of xcp genes, involved in the two-step secretion process, in different Pseudomonas species and other Gram-negative bacteria. Mol. Gen. Genet. 229:278-284.
5. d'Enfert, C., I. Reyss, C. Wandersman, and A. P. Pugsley. 1989. Protein secretion by Gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K12. J. Biol. Chem. 264:17462-17468.
6. Filloux, A., M. Bally, G. Ball, M. Akrim, J. Tommassen, and A. Lazdunski. 1990. Protein secretion in Gram-negative bacteria: transport across the outer membrane involves common mechanism in different bacteria. EMBO J. 9:4323-4329.
7. Gutierrez, C., and J. C. Devedjian. 1989. A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli. Nucleic Acids Res. 17:3999.
8. Hoffman, C. S., and A. Wright. 1985. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion. Proc. Natl. Acad. Sci. USA 82:5107-5111.
9. Liu, P. V. 1974. Extracellular toxins of Pseudomonas aeruginosa. J. Infect. Dis. 130:94-99.
10. Nunn, D., S. Bergman, and S. Lory. 1993. Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J. Bacteriol. 172:2911-2919.
11. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
12. Pugsley, A. P., and I. Reyss. 1990. Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion. Mol. Microbiol. 4:365-379.
13. Reeves, P. J., P. Douglas, and G. P. C. Salmond. 1994. Beta-lactamase topology probe analysis of the OutO NMePhE peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus. Mol. Microbiol. 12:445-457.
14. Sandkvist, M., M. Bagdasarian, S. P. Howard, and V. J. DiRita. 1995. Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J. 14:1664-1673.
15. Schatz, P. J., and J. Beckwith. 1990. Genetic analysis of protein export in Escherichia coli. Annu. Rev. Genet. 24:215-248.
16. Studier, F. W., and B. A. Moffat. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes. J. Mol. Biol. 189:113-130.
17. Tabor, S., and C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
18. Tommassen, J., A. Filloux, M. Bally, M. Murgier, and A. Lazdunski. 1992. Protein secretion in Pseudomonas aeruginosa. FEMS Microbiol. Rev. 103: 73-90.
19. von Heijne, G. 1992. Membrane protein structure prediction. Hydrophobicity analysis and positive-inside rule. J. Mol. Biol. 225:487-494.
20. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBOJ. 8:945-951.
21. Whitley, P., T. Zander, M. Ehrmann, M. Haardt, E. Bremer, and G. von Heijne. 1994. Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW. EMBO J. 13:4653-4661.