Action of defined mixtures of PCBs, p,p′-DDT and its metabolite p,p′-DDE, on co-culture of porcine theca and granulosa cells: Steroid secretion, cell proliferation and apoptosis

Reproductive Toxicology

Volumn 26, Issue 2, 2008, Pages 170-174

  Gregoraszczuk, Ewa L ^a   Ptak, Anna ^a   Karniewska, Magdalena ^a   Ropstad, Erik ^b  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

Cell viability and apoptosis; Combination effects; Estradiol and testosterone secretion; p,p DDT; p,p DDE; PCBs mixtures

Indexed keywords

CASPASE 3; DICHLORODIPHENYLDICHLOROETHYLENE; ESTRADIOL; ORGANIC COMPOUND; POLYBROMINATED DIPHENYLETHER; POLYCHLORINATED BIPHENYL; TESTOSTERONE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL PROLIFERATION; CELL VIABILITY; COCULTURE; ENZYME ACTIVITY; GRANULOSA CELL; NONHUMAN; ORGANIC POLLUTION; OVARY CELL CULTURE; STEROID RELEASE; SWINE; THECA CELL;

ANIMALS; APOPTOSIS; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; COCULTURE TECHNIQUES; DDT; DICHLORODIPHENYL DICHLOROETHYLENE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG SYNERGISM; ENVIRONMENTAL POLLUTANTS; ESTRADIOL; FEMALE; GONADAL STEROID HORMONES; GRANULOSA CELLS; POLYCHLORINATED BIPHENYLS; SWINE; TESTOSTERONE; THECA CELLS;

GADUS MORHUA; SUS;

EID: 53349103037     PISSN: 08906238     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.reprotox.2008.07.003     Document Type: Article

References (34)

1. AMAP, 2002. Persistent Organic Pollutants (POPs) Report 2002. Available: http://www.amap.no/.
2. Wojtowicz A.K., Gregoraszczuk E.L., Lyche J.L., and Ropstad E. Time-dependent and cell-specific action of polychlorinated biphenyls (PCB 153 and PCB 126) on steroid secretion by porcine theca and granulosa cells in mono- and co-culture. J Physiol Pharmacol 51 (2000) 555-568
3. Bonefeld-Jorgensen E.C., Andersen H.R., Rasmussen T.H., and Vinggaard A.M. Effect of highly bioaccumuulated polychlorinated biphenyl congeners on estrogen and androgen receptor activity. Toxicology 14 (2001) 141-153
4. Birnbaum L.S., and Staskal D.F. Brominated flame retardants: cause for concern?. Environ Health Perspect 112 (2004) 9-17
5. Andersen H.R., Vinggaard A.M., Rasmussen T.H., Gjermandsen I.M., and Bonefeld-Jorgensen E.C. Effects of currently used pesticides in assays for estrogenicity, androgenicity, and aromatase activity in vitro. Toxicol Appl Pharmacol 179 (2002) 1-12
6. Wojtowicz A.K., Gregoraszczuk E.L., Ptak A., and Falandysz J. Effect of single and repeated in vitro exposure of ovarian follicles to o,p′-DDT and p,p′-DDT and their metabolites. Pol J Pharmacol 56 (2004) 465-472
7. Woodhouse A.J., and Cooke G.M. Suppression of aromatase activity in vitro by PCBs 28 and 105 and Aroclor 1221. Toxicol Lett 152 (2004) 465-472
8. Gregoraszczuk E.L., Zemla M., Ptak A., and Grabic R. The action of low- and high-chlorinated biphenyl mixture on prepubertal porcine ovary: steroid secretion and cells apoptosis. Endocr Regul 39 (2005) 33-41
9. Cillo F., de Eguileor M., Gandolfi F., and Brevini T.A. Aroclor-1254 affects mRNA polyadenylation, translational activation, cell morphology, and DNA integrity of rat primary prostate cells. Endocr Related Cancer 14 (2007) 257-266
10. Payne J., Rajapakse N., Wilkins M., and Kortenkamp A. Prediction and assessment of the effects of mixtures of four xenoestrogens. Environ Health Perspect 108 (2000) 983-987
11. Ptak A., Ludewig G., Robertson L., Lehmler H., and Gregoraszczuk E. In vitro exposure of porcine prepubertal follicles to 4-chlorobiphenyl (PCB3) and its hydroxylated metabolites: effect on sex hormone levels and aromatase activity. Toxicol Lett 164 (2006) 113-122
12. Hughes S.F., Haney A.F., and Hughes Jr. C.L. Use of human cumulus granulosa cells for in vitro screening of reproductive toxicants. Reprod Toxicol 4 (1990) 11-15
13. Vidal J.D., Vandevoort C.A., Marcus C.B., Lazarewicz N.R., and Conley A.J. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces CYP1B1 expression in human luteinized granulosa cells. Arch Biochem Biophys 439 (2005) 53-60
14. Dasmahapatra A.K., Wimpee B.A., Trewin A.L., and Hutz R.J. 2,3,7,8-Tetrachlorodibenzo-p-dioxin increases steady-state estrogen receptor-beta mRNA levels after CYP1A1 and CYP1B1 induction in rat granulosa cells in vitro. Mol Cell Endocrinol 182 (2001) 39-48
15. Gregoraszczuk E.L., Kajta M., Ptak A., Sowa M., and Wójtowicz A. Effect of PCB 126 and PCB 153 on incidence of apoptosis in cultured theca and granulosa cells collected from small, medium and large preovulatory follicles. Reprod Toxicol 17 (2003) 465-471
16. Augustowska K., Wójtowicz A., Kajta M., Ropstad E., and Gregoraszczuk E.L. Polychlorinated biphenyls (PCB126 and PCB153) action on proliferation and progesterone secretion by cultured in vitro porcine luteal cells. Exp Clin Endocrinol Diabetes 109 (2001) 416-418
17. Tiemann U., Schneider F., Vanselow J., and Tomek W. In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: effects on the biosynthesis of reproductive steroid hormones. Reprod Toxicol 24 (2007) 317-325
18. Stoklosowa S., Gregoraszczuk E., and Channing C. Estrogen and progesterone secretion by isolated cultured porcine thecal and granulosa cells. Biol Reprod 26 (1982) 943-952
19. Tauboll E., Wójtowicz A.K., Ropstad E., and Gregoraszczuk E.L. Valproate irreversibly alters steroid secretion patterns from porcine follicular cells in vitro. Reprod Toxicol 16 (2002) 319-325
20. Gregoraszczuk E.L., Milczarek K., Wójtowicz A., Berg V., Skaare J.U., and Ropstad E. Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs). Reprod Toxicol 25 (2007) 58-66
21. Gregoraszczuk E.L., Grochowalski A., Chrzaszcz R., and Wegiel M. Congener-specific accumulation of polychlorinated biphenyls in ovarian follicular wall follows repeated exposure to PCB 126 and PCB 153. Comparison of tissue levels of PCBs and biological changes. Chemosphere 50 (2003) 481-488
22. Stoklosowa S., Barh J., and Gregoraszczuk E. Some morphological and functional characteristics of cells of the porcine theca interna in tissue culture. Biol Reprod 19 (1978) 712-719
23. Littell R.C., Milliken G.A., Stroup W.W., and Wolfinger R.D. SAS system for mixed models (1996), SAS Institute Inc., Cary, NC
24. Wölfle D. Interactions between 2,3,7,8-TCDD and PCBs as tumor promoters: limitations of TEFs. Teratog Carcinog Mutagen 17 (1997-1998) 217-224
25. Chu I., Lecavalier P., Håkansson H., Yagminas A., Valli V.E., Poon P., and Feeley M. Mixture effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and polychlorinated biphenyl congeners in rats. Chemosphere 43 (2001) 807-814
26. Li L.A., and Wang P.W. PCB126 induces differential changes in androgen, cortisol, and aldosterone biosynthesis in human adrenocortical H295R cells. Toxicol Sci 85 (2005) 530-540
27. Morán F.M., Conley A.J., Corbin C.J., Enan E., VandeVoort C., Overstreet J.W., and Lasley B.L. 2,3,7,8-Tetrachlorodibenzo-p-dioxin decreases estradiol production without altering the enzyme activity of cytochrome P450 aromatase of human luteinized granulosa cells in vitro. Biol Reprod 62 (2000) 1102-1108
28. Oh S.M., Ryu B.T., Lee S.K., and Chung K.H. Antiestrogenic potentials of ortho-PCB congeners by single or complex exposure. Arch Pharm Res 30 (2007) 199-209
29. Gregoraszczuk E.L., and Wójtowicz A.K. In vitro exposure of porcine ovarian follicular cells to PCB 153 alters steroid secretion but not their viability-preliminary study. ScientificWorldJournal 2 (2002) 261-267
30. Wójtowicz A.K., Milewicz T., and Gregoraszczuk E.Ł. Time-dependent action of DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) and its metabolite DDE (1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene) on human chorionic gonadotropin and progesterone secretion. Gynecol Endocrinol 24 (2008) 54-58
31. Rajapakse N., Ong D., and Kortenkamp A. Defining the impact of weakly estrogenic chemicals on the action of steroidal estrogens. Toxicol Sci 60 (2001) 296-304
32. Kortenkamp A., and Altenburger R. Approaches to assessing combination effects of oestrogenic environmental pollutants. Sci Total Environ 233 (1999) 131-140
33. Wójtowicz A.K., Augustowska K., Milewicz T., and Gregoraszczuk E. Differences in the action of o,p-DDE and o,p′-DDD on hormone secretion and cell viability in JEG-3 cell line. Organochalogen Compd 68 (2006) 960-963
34. Gregoraszczuk E.L., Rak A., Kawalec K., and Ropstad E. Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p′-DDT and its metabolite p,p′-DDE. Toxicol Lett (2008) [Epub ahead of print]