A sensitive and reproducible fluorescent-based HPLC assay to measure the activity of acid as well as neutral β-glucocerebrosidases

Analytical Biochemistry

Volumn 383, Issue 1, 2008, Pages 122-129

  Hayashi, Yasuhiro ^a,b   Zama, Kouta ^a,b   Abe, Eriko ^a   Okino, Nozomu ^a   Inoue, Takehiko ^c   Ohno, Kousaku ^c   Ito, Makoto ^a,b  

^a KYUSHU UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c TOTTORI UNIVERSITY   (Japan)

Author keywords

Acid glucocerebrosidase; Fluorescent substrate; Gaucher disease; High performance liquid chromatography; Neutral nonlysosomal glucocerebrosidase

Indexed keywords

CELL CULTURE; CERAMIDES; FIBROBLASTS; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REACTION PRODUCTS; SUBSTRATES;

DIFFERENT SOLVENTS; GAUCHER DISEASE; GLUCOCEREBROSIDASE; GLUCOSYLCERAMIDE; GLYCOSIDIC LINKAGES; HUMAN FIBROBLAST; NORMAL PHASE; RELIABLE METHODS;

DIAGNOSIS;

4 METHYLUMBELLIFERYL BETA DEXTRO GLUCOSIDE; 4 NITROBENZO 2 OXA 1,3 DIAZOLE; 4,4 DIFLUORO 4 BORA 3A,4A DIAZA S INDACENE; ALKENE DERIVATIVE; BETA GLUCOCEREBROSIDASE; CERAMIDE; CONDURITOL B EPOXIDE; ENZYME INHIBITOR; GLUCOSE; GLUCOSIDE; GLUCOSYLCERAMIDASE; OXADIAZOLE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FIBROBLAST; FLUORESCENCE; GAUCHER DISEASE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

VERTEBRATA;

ANIMALS; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ASSAYS; GLUCOSYLCERAMIDASE; HUMANS; RATS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE;

EID: 53049101422     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.07.024     Document Type: Article

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